SULFHYDRYL GROUPS AND THE INTERACTION BETWEEN THE HEMES IN HEMOGLOBIN

1. Dialysis of a hemoglobin solution against slightly alkaline buffer (pH 8.68) causes a decrease in heme-heme interaction. The value of n in Hill's equation drops from 2.9 to 2.0, while the oxygen affinity increases slightly. Addition of glutathione largely reverses the effects of dialysis (n...

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Detalles Bibliográficos
Autor principal: Riggs, Austen F.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1952
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147350/
https://www.ncbi.nlm.nih.gov/pubmed/12981231
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author Riggs, Austen F.
author_facet Riggs, Austen F.
author_sort Riggs, Austen F.
collection PubMed
description 1. Dialysis of a hemoglobin solution against slightly alkaline buffer (pH 8.68) causes a decrease in heme-heme interaction. The value of n in Hill's equation drops from 2.9 to 2.0, while the oxygen affinity increases slightly. Addition of glutathione largely reverses the effects of dialysis (n rises from 2.0 to 2.5 to 2.6). 2. Addition of the sulfhydryl reagent, p-chloromercuribenzoate (1.8 x 10(–3) M), results in a large though not complete blocking of heme-heme interaction. The value of n drops from 2.9 to 1.4. This effect is largely reversed with glutathione (n rises to 2.6).
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spelling pubmed-21473502008-04-23 SULFHYDRYL GROUPS AND THE INTERACTION BETWEEN THE HEMES IN HEMOGLOBIN Riggs, Austen F. J Gen Physiol Article 1. Dialysis of a hemoglobin solution against slightly alkaline buffer (pH 8.68) causes a decrease in heme-heme interaction. The value of n in Hill's equation drops from 2.9 to 2.0, while the oxygen affinity increases slightly. Addition of glutathione largely reverses the effects of dialysis (n rises from 2.0 to 2.5 to 2.6). 2. Addition of the sulfhydryl reagent, p-chloromercuribenzoate (1.8 x 10(–3) M), results in a large though not complete blocking of heme-heme interaction. The value of n drops from 2.9 to 1.4. This effect is largely reversed with glutathione (n rises to 2.6). The Rockefeller University Press 1952-09-20 /pmc/articles/PMC2147350/ /pubmed/12981231 Text en Copyright © Copyright, 1952, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Riggs, Austen F.
SULFHYDRYL GROUPS AND THE INTERACTION BETWEEN THE HEMES IN HEMOGLOBIN
title SULFHYDRYL GROUPS AND THE INTERACTION BETWEEN THE HEMES IN HEMOGLOBIN
title_full SULFHYDRYL GROUPS AND THE INTERACTION BETWEEN THE HEMES IN HEMOGLOBIN
title_fullStr SULFHYDRYL GROUPS AND THE INTERACTION BETWEEN THE HEMES IN HEMOGLOBIN
title_full_unstemmed SULFHYDRYL GROUPS AND THE INTERACTION BETWEEN THE HEMES IN HEMOGLOBIN
title_short SULFHYDRYL GROUPS AND THE INTERACTION BETWEEN THE HEMES IN HEMOGLOBIN
title_sort sulfhydryl groups and the interaction between the hemes in hemoglobin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147350/
https://www.ncbi.nlm.nih.gov/pubmed/12981231
work_keys_str_mv AT riggsaustenf sulfhydrylgroupsandtheinteractionbetweenthehemesinhemoglobin

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