TISSUE CULTURE STUDIES : ISOLATION OF THE ACTIVE COMPONENTS IN THE ULTRAFILTERABLE PORTION OF THE CHICK EMBRYO EXTRACT

A fraction of the ultrafilterable portion of chick embryo extract was isolated by alcohol extraction of a lyophilized powder of the ultrafiltrate followed by ion exchange removal of many of the inert components of the alcohol extract. This fraction contained 3 per cent of the ultrafilterable nitroge...

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Detalles Bibliográficos
Autores principales: Rosenberg, Sheldon, Zitcer, Elsa, Kirk, Paul L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1953
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147430/
https://www.ncbi.nlm.nih.gov/pubmed/13109158
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author Rosenberg, Sheldon
Zitcer, Elsa
Kirk, Paul L.
author_facet Rosenberg, Sheldon
Zitcer, Elsa
Kirk, Paul L.
author_sort Rosenberg, Sheldon
collection PubMed
description A fraction of the ultrafilterable portion of chick embryo extract was isolated by alcohol extraction of a lyophilized powder of the ultrafiltrate followed by ion exchange removal of many of the inert components of the alcohol extract. This fraction contained 3 per cent of the ultrafilterable nitrogen but was capable of completely restoring the growth-promoting activity of dialyzed embryo extract, when tested with chick heart fibroblasts in roller tube cultures. The low nitrogen content, shape of the ultraviolet absorption spectrum, and presence of few free amino acids, suggest that non-dialyzable compounds serve as the chief source of nutrition for this system.
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spelling pubmed-21474302008-04-23 TISSUE CULTURE STUDIES : ISOLATION OF THE ACTIVE COMPONENTS IN THE ULTRAFILTERABLE PORTION OF THE CHICK EMBRYO EXTRACT Rosenberg, Sheldon Zitcer, Elsa Kirk, Paul L. J Gen Physiol Article A fraction of the ultrafilterable portion of chick embryo extract was isolated by alcohol extraction of a lyophilized powder of the ultrafiltrate followed by ion exchange removal of many of the inert components of the alcohol extract. This fraction contained 3 per cent of the ultrafilterable nitrogen but was capable of completely restoring the growth-promoting activity of dialyzed embryo extract, when tested with chick heart fibroblasts in roller tube cultures. The low nitrogen content, shape of the ultraviolet absorption spectrum, and presence of few free amino acids, suggest that non-dialyzable compounds serve as the chief source of nutrition for this system. The Rockefeller University Press 1953-11-20 /pmc/articles/PMC2147430/ /pubmed/13109158 Text en Copyright © Copyright, 1953, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Rosenberg, Sheldon
Zitcer, Elsa
Kirk, Paul L.
TISSUE CULTURE STUDIES : ISOLATION OF THE ACTIVE COMPONENTS IN THE ULTRAFILTERABLE PORTION OF THE CHICK EMBRYO EXTRACT
title TISSUE CULTURE STUDIES : ISOLATION OF THE ACTIVE COMPONENTS IN THE ULTRAFILTERABLE PORTION OF THE CHICK EMBRYO EXTRACT
title_full TISSUE CULTURE STUDIES : ISOLATION OF THE ACTIVE COMPONENTS IN THE ULTRAFILTERABLE PORTION OF THE CHICK EMBRYO EXTRACT
title_fullStr TISSUE CULTURE STUDIES : ISOLATION OF THE ACTIVE COMPONENTS IN THE ULTRAFILTERABLE PORTION OF THE CHICK EMBRYO EXTRACT
title_full_unstemmed TISSUE CULTURE STUDIES : ISOLATION OF THE ACTIVE COMPONENTS IN THE ULTRAFILTERABLE PORTION OF THE CHICK EMBRYO EXTRACT
title_short TISSUE CULTURE STUDIES : ISOLATION OF THE ACTIVE COMPONENTS IN THE ULTRAFILTERABLE PORTION OF THE CHICK EMBRYO EXTRACT
title_sort tissue culture studies : isolation of the active components in the ultrafilterable portion of the chick embryo extract
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147430/
https://www.ncbi.nlm.nih.gov/pubmed/13109158
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AT kirkpaull tissueculturestudiesisolationoftheactivecomponentsintheultrafilterableportionofthechickembryoextract

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