ISOLATION OF PURIFIED COPPER PROTEIN FROM HORSE LIVER

1. A procedure is described for isolating a copper protein from horse liver in pure form, in a state that appears to be crystalline. 2. This copper protein is colored blue-green, and its copper content varied between 0.3 and 0.4 per cent. No indication of any catalytic properties was obtained in the...

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Detalles Bibliográficos
Autores principales: Mohamed, Mostafa S., Greenberg, David M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1954
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147441/
https://www.ncbi.nlm.nih.gov/pubmed/13143179
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author Mohamed, Mostafa S.
Greenberg, David M.
author_facet Mohamed, Mostafa S.
Greenberg, David M.
author_sort Mohamed, Mostafa S.
collection PubMed
description 1. A procedure is described for isolating a copper protein from horse liver in pure form, in a state that appears to be crystalline. 2. This copper protein is colored blue-green, and its copper content varied between 0.3 and 0.4 per cent. No indication of any catalytic properties was obtained in the limited number of tests made. Because of its variable copper content, the protein may function for the storage of copper in the body. 3. The copper could be removed partly from the protein by dialysis against cyanide, and a colorless crystalline protein was obtained by lowering the pH to 5.0, followed by dialysis. The original blue-green color of the protein could be restored by dialysis against copper acetate solution.
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spelling pubmed-21474412008-04-23 ISOLATION OF PURIFIED COPPER PROTEIN FROM HORSE LIVER Mohamed, Mostafa S. Greenberg, David M. J Gen Physiol Article 1. A procedure is described for isolating a copper protein from horse liver in pure form, in a state that appears to be crystalline. 2. This copper protein is colored blue-green, and its copper content varied between 0.3 and 0.4 per cent. No indication of any catalytic properties was obtained in the limited number of tests made. Because of its variable copper content, the protein may function for the storage of copper in the body. 3. The copper could be removed partly from the protein by dialysis against cyanide, and a colorless crystalline protein was obtained by lowering the pH to 5.0, followed by dialysis. The original blue-green color of the protein could be restored by dialysis against copper acetate solution. The Rockefeller University Press 1954-03-20 /pmc/articles/PMC2147441/ /pubmed/13143179 Text en Copyright © Copyright, 1954, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Mohamed, Mostafa S.
Greenberg, David M.
ISOLATION OF PURIFIED COPPER PROTEIN FROM HORSE LIVER
title ISOLATION OF PURIFIED COPPER PROTEIN FROM HORSE LIVER
title_full ISOLATION OF PURIFIED COPPER PROTEIN FROM HORSE LIVER
title_fullStr ISOLATION OF PURIFIED COPPER PROTEIN FROM HORSE LIVER
title_full_unstemmed ISOLATION OF PURIFIED COPPER PROTEIN FROM HORSE LIVER
title_short ISOLATION OF PURIFIED COPPER PROTEIN FROM HORSE LIVER
title_sort isolation of purified copper protein from horse liver
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147441/
https://www.ncbi.nlm.nih.gov/pubmed/13143179
work_keys_str_mv AT mohamedmostafas isolationofpurifiedcopperproteinfromhorseliver
AT greenbergdavidm isolationofpurifiedcopperproteinfromhorseliver

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