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PHOTODYNAMIC HEMOLYSIS AT LOW TEMPERATURES
It is shown that photodynamic hemolysis may occur at –79°C. if the erythrocytes are suspended in a solution containing 70 per cent glycerol which prevents hemolysis by freezing; but that there is no hemolysis under the same conditions at –210°C. At the higher temperature the viscosity of the solutio...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1954
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147457/ https://www.ncbi.nlm.nih.gov/pubmed/13118101 |
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author | Blum, Harold F. Kauzmann, Elizabeth Flagler |
author_facet | Blum, Harold F. Kauzmann, Elizabeth Flagler |
author_sort | Blum, Harold F. |
collection | PubMed |
description | It is shown that photodynamic hemolysis may occur at –79°C. if the erythrocytes are suspended in a solution containing 70 per cent glycerol which prevents hemolysis by freezing; but that there is no hemolysis under the same conditions at –210°C. At the higher temperature the viscosity of the solution is still low enough to permit appreciable movement of molecules, whereas at the lower temperature the molecules must be virtually immobile. The findings are compatible with the idea that the dye molecule acts in a cycle, bringing about successive oxidations by O(2) molecules, as has been shown for photodynamic hemolysis at room temperature. The assumption of a combination between dye, O(2), and substrate does not explain photosensitized hemolysis in the semi-solid state. The mechanism of photosensitized oxidation by O(2) is discussed. |
format | Text |
id | pubmed-2147457 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1954 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21474572008-04-23 PHOTODYNAMIC HEMOLYSIS AT LOW TEMPERATURES Blum, Harold F. Kauzmann, Elizabeth Flagler J Gen Physiol Article It is shown that photodynamic hemolysis may occur at –79°C. if the erythrocytes are suspended in a solution containing 70 per cent glycerol which prevents hemolysis by freezing; but that there is no hemolysis under the same conditions at –210°C. At the higher temperature the viscosity of the solution is still low enough to permit appreciable movement of molecules, whereas at the lower temperature the molecules must be virtually immobile. The findings are compatible with the idea that the dye molecule acts in a cycle, bringing about successive oxidations by O(2) molecules, as has been shown for photodynamic hemolysis at room temperature. The assumption of a combination between dye, O(2), and substrate does not explain photosensitized hemolysis in the semi-solid state. The mechanism of photosensitized oxidation by O(2) is discussed. The Rockefeller University Press 1954-01-20 /pmc/articles/PMC2147457/ /pubmed/13118101 Text en Copyright © Copyright, 1954, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Blum, Harold F. Kauzmann, Elizabeth Flagler PHOTODYNAMIC HEMOLYSIS AT LOW TEMPERATURES |
title | PHOTODYNAMIC HEMOLYSIS AT LOW TEMPERATURES |
title_full | PHOTODYNAMIC HEMOLYSIS AT LOW TEMPERATURES |
title_fullStr | PHOTODYNAMIC HEMOLYSIS AT LOW TEMPERATURES |
title_full_unstemmed | PHOTODYNAMIC HEMOLYSIS AT LOW TEMPERATURES |
title_short | PHOTODYNAMIC HEMOLYSIS AT LOW TEMPERATURES |
title_sort | photodynamic hemolysis at low temperatures |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147457/ https://www.ncbi.nlm.nih.gov/pubmed/13118101 |
work_keys_str_mv | AT blumharoldf photodynamichemolysisatlowtemperatures AT kauzmannelizabethflagler photodynamichemolysisatlowtemperatures |