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ANALYSIS OF RESPIRATION DURING GERMINATION AND ENLARGEMENT OF SPORES OF BACILLUS MEGATERIUM AND OF THE FUNGUS MYROTHECIUM VERRUCARIA

1. There are certainly two, and probably three, stages in the development of B. megaterium from the spore to inception of cell division. The rapid increase in rate of respiration during the initial 10 minutes on glucose-peptone-yeast extract medium coincides with decrease in optical density and with...

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Detalles Bibliográficos
Autores principales: Mandels, G. R., Levinson, H. S., Hyatt, M. T.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1956
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147541/
https://www.ncbi.nlm.nih.gov/pubmed/13286449
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author Mandels, G. R.
Levinson, H. S.
Hyatt, M. T.
author_facet Mandels, G. R.
Levinson, H. S.
Hyatt, M. T.
author_sort Mandels, G. R.
collection PubMed
description 1. There are certainly two, and probably three, stages in the development of B. megaterium from the spore to inception of cell division. The rapid increase in rate of respiration during the initial 10 minutes on glucose-peptone-yeast extract medium coincides with decrease in optical density and with increase in stainability. From about 10 to 100 minutes, the rate increases linearly, coinciding with swelling of the spores and ending at approximately the time of rupture of the spore case. From about 100 to 180 minutes, there is a second and steeper linear increase in respiration rate coinciding with cell elongation. These physiological and morphological phenomena are discussed as criteria for germination. 2. The rate of respiration of M. verrucaria spores also increases linearly up to about 300 minutes in sucrose-yeast extract medium. No breaks in the curves are observed during formation of the germ tubes. 3. Oxygen uptake follows the parabolic curve See PDF for Equation within the limits of experimental error for both types of spores. 4. It is postulated that metabolism during these stages of linear increase may be regulated by processes occurring at cellular or intracellular surfaces or by synthesis of a limiting enzyme at constant rate.
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spelling pubmed-21475412008-04-23 ANALYSIS OF RESPIRATION DURING GERMINATION AND ENLARGEMENT OF SPORES OF BACILLUS MEGATERIUM AND OF THE FUNGUS MYROTHECIUM VERRUCARIA Mandels, G. R. Levinson, H. S. Hyatt, M. T. J Gen Physiol Article 1. There are certainly two, and probably three, stages in the development of B. megaterium from the spore to inception of cell division. The rapid increase in rate of respiration during the initial 10 minutes on glucose-peptone-yeast extract medium coincides with decrease in optical density and with increase in stainability. From about 10 to 100 minutes, the rate increases linearly, coinciding with swelling of the spores and ending at approximately the time of rupture of the spore case. From about 100 to 180 minutes, there is a second and steeper linear increase in respiration rate coinciding with cell elongation. These physiological and morphological phenomena are discussed as criteria for germination. 2. The rate of respiration of M. verrucaria spores also increases linearly up to about 300 minutes in sucrose-yeast extract medium. No breaks in the curves are observed during formation of the germ tubes. 3. Oxygen uptake follows the parabolic curve See PDF for Equation within the limits of experimental error for both types of spores. 4. It is postulated that metabolism during these stages of linear increase may be regulated by processes occurring at cellular or intracellular surfaces or by synthesis of a limiting enzyme at constant rate. The Rockefeller University Press 1956-01-20 /pmc/articles/PMC2147541/ /pubmed/13286449 Text en Copyright © Copyright, 1956, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Mandels, G. R.
Levinson, H. S.
Hyatt, M. T.
ANALYSIS OF RESPIRATION DURING GERMINATION AND ENLARGEMENT OF SPORES OF BACILLUS MEGATERIUM AND OF THE FUNGUS MYROTHECIUM VERRUCARIA
title ANALYSIS OF RESPIRATION DURING GERMINATION AND ENLARGEMENT OF SPORES OF BACILLUS MEGATERIUM AND OF THE FUNGUS MYROTHECIUM VERRUCARIA
title_full ANALYSIS OF RESPIRATION DURING GERMINATION AND ENLARGEMENT OF SPORES OF BACILLUS MEGATERIUM AND OF THE FUNGUS MYROTHECIUM VERRUCARIA
title_fullStr ANALYSIS OF RESPIRATION DURING GERMINATION AND ENLARGEMENT OF SPORES OF BACILLUS MEGATERIUM AND OF THE FUNGUS MYROTHECIUM VERRUCARIA
title_full_unstemmed ANALYSIS OF RESPIRATION DURING GERMINATION AND ENLARGEMENT OF SPORES OF BACILLUS MEGATERIUM AND OF THE FUNGUS MYROTHECIUM VERRUCARIA
title_short ANALYSIS OF RESPIRATION DURING GERMINATION AND ENLARGEMENT OF SPORES OF BACILLUS MEGATERIUM AND OF THE FUNGUS MYROTHECIUM VERRUCARIA
title_sort analysis of respiration during germination and enlargement of spores of bacillus megaterium and of the fungus myrothecium verrucaria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147541/
https://www.ncbi.nlm.nih.gov/pubmed/13286449
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