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Dynamic Localization of CLIP-170 to Microtubule Plus Ends Is Coupled to Microtubule Assembly
CLIP-170 is a cytoplasmic linker protein that localizes to plus ends of microtubules in vivo. In this study, we have characterized the microtubule-binding properties of CLIP-170, to understand the mechanism of its plus end targeting. We show that the NH(2)-terminal microtubule-interacting domain of...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1999
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2148115/ https://www.ncbi.nlm.nih.gov/pubmed/9885247 |
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author | Diamantopoulos, Georgios S. Perez, Franck Goodson, Holly V. Batelier, Gérard Melki, Ronald Kreis, Thomas E. Rickard, Janet E. |
author_facet | Diamantopoulos, Georgios S. Perez, Franck Goodson, Holly V. Batelier, Gérard Melki, Ronald Kreis, Thomas E. Rickard, Janet E. |
author_sort | Diamantopoulos, Georgios S. |
collection | PubMed |
description | CLIP-170 is a cytoplasmic linker protein that localizes to plus ends of microtubules in vivo. In this study, we have characterized the microtubule-binding properties of CLIP-170, to understand the mechanism of its plus end targeting. We show that the NH(2)-terminal microtubule-interacting domain of CLIP-170 alone localizes to microtubule plus ends when transfected into cells. Association of CLIP-170 with newly-formed microtubules was observed in cells microinjected with biotinylated tubulin, used as a tracer for growing microtubules. Using in vitro assays, association of CLIP-170 with recently polymerized tubulin is also seen. Cross-linking and sedimentation velocity experiments suggest association of CLIP-170 with nonpolymerized tubulin. We conclude from these experiments that the microtubule end targeting of CLIP-170 is closely linked to tubulin polymerization. |
format | Text |
id | pubmed-2148115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1999 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21481152008-05-01 Dynamic Localization of CLIP-170 to Microtubule Plus Ends Is Coupled to Microtubule Assembly Diamantopoulos, Georgios S. Perez, Franck Goodson, Holly V. Batelier, Gérard Melki, Ronald Kreis, Thomas E. Rickard, Janet E. J Cell Biol Article CLIP-170 is a cytoplasmic linker protein that localizes to plus ends of microtubules in vivo. In this study, we have characterized the microtubule-binding properties of CLIP-170, to understand the mechanism of its plus end targeting. We show that the NH(2)-terminal microtubule-interacting domain of CLIP-170 alone localizes to microtubule plus ends when transfected into cells. Association of CLIP-170 with newly-formed microtubules was observed in cells microinjected with biotinylated tubulin, used as a tracer for growing microtubules. Using in vitro assays, association of CLIP-170 with recently polymerized tubulin is also seen. Cross-linking and sedimentation velocity experiments suggest association of CLIP-170 with nonpolymerized tubulin. We conclude from these experiments that the microtubule end targeting of CLIP-170 is closely linked to tubulin polymerization. The Rockefeller University Press 1999-01-11 /pmc/articles/PMC2148115/ /pubmed/9885247 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Diamantopoulos, Georgios S. Perez, Franck Goodson, Holly V. Batelier, Gérard Melki, Ronald Kreis, Thomas E. Rickard, Janet E. Dynamic Localization of CLIP-170 to Microtubule Plus Ends Is Coupled to Microtubule Assembly |
title | Dynamic Localization of CLIP-170 to Microtubule Plus Ends Is Coupled to Microtubule Assembly |
title_full | Dynamic Localization of CLIP-170 to Microtubule Plus Ends Is Coupled to Microtubule Assembly |
title_fullStr | Dynamic Localization of CLIP-170 to Microtubule Plus Ends Is Coupled to Microtubule Assembly |
title_full_unstemmed | Dynamic Localization of CLIP-170 to Microtubule Plus Ends Is Coupled to Microtubule Assembly |
title_short | Dynamic Localization of CLIP-170 to Microtubule Plus Ends Is Coupled to Microtubule Assembly |
title_sort | dynamic localization of clip-170 to microtubule plus ends is coupled to microtubule assembly |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2148115/ https://www.ncbi.nlm.nih.gov/pubmed/9885247 |
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