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Drosophila Polo Kinase Is Required for Cytokinesis
A number of lines of evidence point to a predominance of cytokinesis defects in spermatogenesis in hypomorphic alleles of the Drosophila polo gene. In the pre-meiotic mitoses, cytokinesis defects result in cysts of primary spermatocytes with reduced numbers of cells that can contain multiple centros...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1998
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2148135/ https://www.ncbi.nlm.nih.gov/pubmed/9813088 |
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author | Carmena, Mar Riparbelli, Maria Giovanna Minestrini, Gianluca Tavares, Álvaro M. Adams, Richard Callaini, Giuliano Glover, David M. |
author_facet | Carmena, Mar Riparbelli, Maria Giovanna Minestrini, Gianluca Tavares, Álvaro M. Adams, Richard Callaini, Giuliano Glover, David M. |
author_sort | Carmena, Mar |
collection | PubMed |
description | A number of lines of evidence point to a predominance of cytokinesis defects in spermatogenesis in hypomorphic alleles of the Drosophila polo gene. In the pre-meiotic mitoses, cytokinesis defects result in cysts of primary spermatocytes with reduced numbers of cells that can contain multiple centrosomes. These are connected by a correspondingly reduced number of ring canals, structures formed by the stabilization of the cleavage furrow. The earliest defects during the meiotic divisions are a failure to form the correct mid-zone and mid-body structures at telophase. This is accompanied by a failure to correctly localize the Pavarotti kinesin- like protein that functions in cytokinesis, and of the septin Peanut and of actin to be incorporated into a contractile ring. In spite of these defects, cyclin B is degraded and the cells exit M phase. The resulting spermatids are frequently binuclear or tetranuclear, in which case they develop either two or four axonemes, respectively. A significant proportion of spermatids in which cytokinesis has failed may also show the segregation defects previously ascribed to polo(1) mutants. We discuss these findings in respect to conserved functions for the Polo-like kinases in regulating progression through M phase, including the earliest events of cytokinesis. |
format | Text |
id | pubmed-2148135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1998 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21481352008-05-01 Drosophila Polo Kinase Is Required for Cytokinesis Carmena, Mar Riparbelli, Maria Giovanna Minestrini, Gianluca Tavares, Álvaro M. Adams, Richard Callaini, Giuliano Glover, David M. J Cell Biol Regular Articles A number of lines of evidence point to a predominance of cytokinesis defects in spermatogenesis in hypomorphic alleles of the Drosophila polo gene. In the pre-meiotic mitoses, cytokinesis defects result in cysts of primary spermatocytes with reduced numbers of cells that can contain multiple centrosomes. These are connected by a correspondingly reduced number of ring canals, structures formed by the stabilization of the cleavage furrow. The earliest defects during the meiotic divisions are a failure to form the correct mid-zone and mid-body structures at telophase. This is accompanied by a failure to correctly localize the Pavarotti kinesin- like protein that functions in cytokinesis, and of the septin Peanut and of actin to be incorporated into a contractile ring. In spite of these defects, cyclin B is degraded and the cells exit M phase. The resulting spermatids are frequently binuclear or tetranuclear, in which case they develop either two or four axonemes, respectively. A significant proportion of spermatids in which cytokinesis has failed may also show the segregation defects previously ascribed to polo(1) mutants. We discuss these findings in respect to conserved functions for the Polo-like kinases in regulating progression through M phase, including the earliest events of cytokinesis. The Rockefeller University Press 1998-11-02 /pmc/articles/PMC2148135/ /pubmed/9813088 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Regular Articles Carmena, Mar Riparbelli, Maria Giovanna Minestrini, Gianluca Tavares, Álvaro M. Adams, Richard Callaini, Giuliano Glover, David M. Drosophila Polo Kinase Is Required for Cytokinesis |
title |
Drosophila Polo Kinase Is Required for Cytokinesis |
title_full |
Drosophila Polo Kinase Is Required for Cytokinesis |
title_fullStr |
Drosophila Polo Kinase Is Required for Cytokinesis |
title_full_unstemmed |
Drosophila Polo Kinase Is Required for Cytokinesis |
title_short |
Drosophila Polo Kinase Is Required for Cytokinesis |
title_sort | drosophila polo kinase is required for cytokinesis |
topic | Regular Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2148135/ https://www.ncbi.nlm.nih.gov/pubmed/9813088 |
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