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Dystrophic Muscle in Mice Chimeric for Expression of α5 Integrin

α5-deficient mice die early in embryogenesis (Yang et al., 1993). To study the functions of α5 integrin later in mouse embryogenesis and during adult life we generated α5 −/−;+/+ chimeric mice. These animals contain α5-negative and positive cells randomly distributed. Analysis of the chimerism by gl...

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Autores principales: Taverna, Daniela, Disatnik, Marie-Helene, Rayburn, Helen, Bronson, Roderick T., Yang, Joy, Rando, Thomas A., Hynes, Richard O.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2148145/
https://www.ncbi.nlm.nih.gov/pubmed/9813102
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author Taverna, Daniela
Disatnik, Marie-Helene
Rayburn, Helen
Bronson, Roderick T.
Yang, Joy
Rando, Thomas A.
Hynes, Richard O.
author_facet Taverna, Daniela
Disatnik, Marie-Helene
Rayburn, Helen
Bronson, Roderick T.
Yang, Joy
Rando, Thomas A.
Hynes, Richard O.
author_sort Taverna, Daniela
collection PubMed
description α5-deficient mice die early in embryogenesis (Yang et al., 1993). To study the functions of α5 integrin later in mouse embryogenesis and during adult life we generated α5 −/−;+/+ chimeric mice. These animals contain α5-negative and positive cells randomly distributed. Analysis of the chimerism by glucose- 6-phosphate isomerase (GPI) assay revealed that α5 −/− cells contributed to all the tissues analyzed. High contributions were observed in the skeletal muscle. The perinatal survival of the mutant chimeras was lower than for the controls, however the subsequent life span of the survivors was only slightly reduced compared with controls (Taverna et al., 1998). Histological analysis of α5 −/−;+/+ mice from late embryogenesis to adult life revealed an alteration in the skeletal muscle structure resembling a typical muscle dystrophy. Giant fibers, increased numbers of nuclei per fiber with altered position and size, vacuoli and signs of muscle degeneration–regeneration were observed in head, thorax and limb muscles. Electron microscopy showed an increase in the number of mitochondria in some muscle fibers of the mutant mice. Increased apoptosis and immunoreactivity for tenascin-C were observed in mutant muscle fibers. All the alterations were already visible at late stages of embryogenesis. The number of altered muscle fibers varied in different animals and muscles and was often increased in high percentage chimeric animals. Differentiation of α5 −/− ES cells or myoblasts showed that in vitro differentiation into myotubes was achieved normally. However proper adhesion and survival of myoblasts on fibronectin was impaired. Our data suggest that a novel form of muscle dystrophy in mice is α5-integrin-dependent.
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spelling pubmed-21481452008-05-01 Dystrophic Muscle in Mice Chimeric for Expression of α5 Integrin Taverna, Daniela Disatnik, Marie-Helene Rayburn, Helen Bronson, Roderick T. Yang, Joy Rando, Thomas A. Hynes, Richard O. J Cell Biol Regular Articles α5-deficient mice die early in embryogenesis (Yang et al., 1993). To study the functions of α5 integrin later in mouse embryogenesis and during adult life we generated α5 −/−;+/+ chimeric mice. These animals contain α5-negative and positive cells randomly distributed. Analysis of the chimerism by glucose- 6-phosphate isomerase (GPI) assay revealed that α5 −/− cells contributed to all the tissues analyzed. High contributions were observed in the skeletal muscle. The perinatal survival of the mutant chimeras was lower than for the controls, however the subsequent life span of the survivors was only slightly reduced compared with controls (Taverna et al., 1998). Histological analysis of α5 −/−;+/+ mice from late embryogenesis to adult life revealed an alteration in the skeletal muscle structure resembling a typical muscle dystrophy. Giant fibers, increased numbers of nuclei per fiber with altered position and size, vacuoli and signs of muscle degeneration–regeneration were observed in head, thorax and limb muscles. Electron microscopy showed an increase in the number of mitochondria in some muscle fibers of the mutant mice. Increased apoptosis and immunoreactivity for tenascin-C were observed in mutant muscle fibers. All the alterations were already visible at late stages of embryogenesis. The number of altered muscle fibers varied in different animals and muscles and was often increased in high percentage chimeric animals. Differentiation of α5 −/− ES cells or myoblasts showed that in vitro differentiation into myotubes was achieved normally. However proper adhesion and survival of myoblasts on fibronectin was impaired. Our data suggest that a novel form of muscle dystrophy in mice is α5-integrin-dependent. The Rockefeller University Press 1998-11-02 /pmc/articles/PMC2148145/ /pubmed/9813102 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Regular Articles
Taverna, Daniela
Disatnik, Marie-Helene
Rayburn, Helen
Bronson, Roderick T.
Yang, Joy
Rando, Thomas A.
Hynes, Richard O.
Dystrophic Muscle in Mice Chimeric for Expression of α5 Integrin
title Dystrophic Muscle in Mice Chimeric for Expression of α5 Integrin
title_full Dystrophic Muscle in Mice Chimeric for Expression of α5 Integrin
title_fullStr Dystrophic Muscle in Mice Chimeric for Expression of α5 Integrin
title_full_unstemmed Dystrophic Muscle in Mice Chimeric for Expression of α5 Integrin
title_short Dystrophic Muscle in Mice Chimeric for Expression of α5 Integrin
title_sort dystrophic muscle in mice chimeric for expression of α5 integrin
topic Regular Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2148145/
https://www.ncbi.nlm.nih.gov/pubmed/9813102
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