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SNARE Membrane Trafficking Dynamics In Vivo

The ER/Golgi soluble NSF attachment protein receptor (SNARE) membrin, rsec22b, and rbet1 are enriched in ∼1-μm cytoplasmic structures that lie very close to the ER. These appear to be ER exit sites since secretory cargo concentrates in and exits from these structures. rsec22b and rbet1 fused to fluo...

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Autores principales: Chao, Daniel S., Hay, Jesse C., Winnick, Shawn, Prekeris, Rytis, Klumperman, Judith, Scheller, Richard H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2148207/
https://www.ncbi.nlm.nih.gov/pubmed/10085287
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author Chao, Daniel S.
Hay, Jesse C.
Winnick, Shawn
Prekeris, Rytis
Klumperman, Judith
Scheller, Richard H.
author_facet Chao, Daniel S.
Hay, Jesse C.
Winnick, Shawn
Prekeris, Rytis
Klumperman, Judith
Scheller, Richard H.
author_sort Chao, Daniel S.
collection PubMed
description The ER/Golgi soluble NSF attachment protein receptor (SNARE) membrin, rsec22b, and rbet1 are enriched in ∼1-μm cytoplasmic structures that lie very close to the ER. These appear to be ER exit sites since secretory cargo concentrates in and exits from these structures. rsec22b and rbet1 fused to fluorescent proteins are enriched at ∼1-μm ER exit sites that remained more or less stationary, but periodically emitted streaks of fluorescence that traveled generally in the direction of the Golgi complex. These exit sites were reused and subsequent tubules or streams of vesicles followed similar trajectories. Fluorescent membrin- enriched ∼1-μm peripheral structures were more mobile and appeared to translocate through the cytoplasm back and forth, between the periphery and the Golgi area. These mobile structures could serve to collect secretory cargo by fusing with ER-derived vesicles and ferrying the cargo to the Golgi. The post-Golgi SNAREs, syntaxin 6 and syntaxin 13, when fused to fluorescent proteins each displayed characteristic patterns of movement. However, syntaxin 13 was the only SNARE whose life cycle appeared to involve interactions with the plasma membrane. These studies reveal the in vivo spatiotemporal dynamics of SNARE proteins and provide new insight into their roles in membrane trafficking.
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spelling pubmed-21482072008-05-01 SNARE Membrane Trafficking Dynamics In Vivo Chao, Daniel S. Hay, Jesse C. Winnick, Shawn Prekeris, Rytis Klumperman, Judith Scheller, Richard H. J Cell Biol Regular Articles The ER/Golgi soluble NSF attachment protein receptor (SNARE) membrin, rsec22b, and rbet1 are enriched in ∼1-μm cytoplasmic structures that lie very close to the ER. These appear to be ER exit sites since secretory cargo concentrates in and exits from these structures. rsec22b and rbet1 fused to fluorescent proteins are enriched at ∼1-μm ER exit sites that remained more or less stationary, but periodically emitted streaks of fluorescence that traveled generally in the direction of the Golgi complex. These exit sites were reused and subsequent tubules or streams of vesicles followed similar trajectories. Fluorescent membrin- enriched ∼1-μm peripheral structures were more mobile and appeared to translocate through the cytoplasm back and forth, between the periphery and the Golgi area. These mobile structures could serve to collect secretory cargo by fusing with ER-derived vesicles and ferrying the cargo to the Golgi. The post-Golgi SNAREs, syntaxin 6 and syntaxin 13, when fused to fluorescent proteins each displayed characteristic patterns of movement. However, syntaxin 13 was the only SNARE whose life cycle appeared to involve interactions with the plasma membrane. These studies reveal the in vivo spatiotemporal dynamics of SNARE proteins and provide new insight into their roles in membrane trafficking. The Rockefeller University Press 1999-03-08 /pmc/articles/PMC2148207/ /pubmed/10085287 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Regular Articles
Chao, Daniel S.
Hay, Jesse C.
Winnick, Shawn
Prekeris, Rytis
Klumperman, Judith
Scheller, Richard H.
SNARE Membrane Trafficking Dynamics In Vivo
title SNARE Membrane Trafficking Dynamics In Vivo
title_full SNARE Membrane Trafficking Dynamics In Vivo
title_fullStr SNARE Membrane Trafficking Dynamics In Vivo
title_full_unstemmed SNARE Membrane Trafficking Dynamics In Vivo
title_short SNARE Membrane Trafficking Dynamics In Vivo
title_sort snare membrane trafficking dynamics in vivo
topic Regular Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2148207/
https://www.ncbi.nlm.nih.gov/pubmed/10085287
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