Assay of matrix metalloproteinases types 1, 2, 3 and 9 in breast cancer.

Matrix metalloproteinases (MMPs) are zinc dependent endopeptidases implicated in cancer invasion and metastasis. Gelatin zymography was performed on 84 human breast carcinomas and seven normal breast tissues. The precursor form of MMP-2 (72 kDa) was found in 11 (12%) samples, while its two activated...

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Autores principales: Remacle, A. G., Noël, A., Duggan, C., McDermott, E., O'Higgins, N., Foidart, J. M., Duffy, M. J.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1998
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150110/
https://www.ncbi.nlm.nih.gov/pubmed/9528836
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author Remacle, A. G.
Noël, A.
Duggan, C.
McDermott, E.
O'Higgins, N.
Foidart, J. M.
Duffy, M. J.
author_facet Remacle, A. G.
Noël, A.
Duggan, C.
McDermott, E.
O'Higgins, N.
Foidart, J. M.
Duffy, M. J.
author_sort Remacle, A. G.
collection PubMed
description Matrix metalloproteinases (MMPs) are zinc dependent endopeptidases implicated in cancer invasion and metastasis. Gelatin zymography was performed on 84 human breast carcinomas and seven normal breast tissues. The precursor form of MMP-2 (72 kDa) was found in 11 (12%) samples, while its two activated forms, i.e. 62 kDa and 59 kDa, were found in three (6%) and 34 (40%) samples respectively. In contrast to MMP-2, most of the samples (52%) contained MMP-9 in its precursor form. Using ELISA, MMP-1 levels were found in 12% of the samples while MMP-3 levels were found in only 2% of the samples. Levels of MMP-2, -3 and -9 correlated inversely with numbers of nodal metastases. Neither MMP-2 nor -9 levels were significantly related to patient outcome. However, patients with high levels of a 50-kDa gelatinase band after zymography had a significantly better survival than patients with low levels. This species was never observed in normal breast tissue. IMAGES:
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spelling pubmed-21501102009-09-10 Assay of matrix metalloproteinases types 1, 2, 3 and 9 in breast cancer. Remacle, A. G. Noël, A. Duggan, C. McDermott, E. O'Higgins, N. Foidart, J. M. Duffy, M. J. Br J Cancer Research Article Matrix metalloproteinases (MMPs) are zinc dependent endopeptidases implicated in cancer invasion and metastasis. Gelatin zymography was performed on 84 human breast carcinomas and seven normal breast tissues. The precursor form of MMP-2 (72 kDa) was found in 11 (12%) samples, while its two activated forms, i.e. 62 kDa and 59 kDa, were found in three (6%) and 34 (40%) samples respectively. In contrast to MMP-2, most of the samples (52%) contained MMP-9 in its precursor form. Using ELISA, MMP-1 levels were found in 12% of the samples while MMP-3 levels were found in only 2% of the samples. Levels of MMP-2, -3 and -9 correlated inversely with numbers of nodal metastases. Neither MMP-2 nor -9 levels were significantly related to patient outcome. However, patients with high levels of a 50-kDa gelatinase band after zymography had a significantly better survival than patients with low levels. This species was never observed in normal breast tissue. IMAGES: Nature Publishing Group 1998-03 /pmc/articles/PMC2150110/ /pubmed/9528836 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Remacle, A. G.
Noël, A.
Duggan, C.
McDermott, E.
O'Higgins, N.
Foidart, J. M.
Duffy, M. J.
Assay of matrix metalloproteinases types 1, 2, 3 and 9 in breast cancer.
title Assay of matrix metalloproteinases types 1, 2, 3 and 9 in breast cancer.
title_full Assay of matrix metalloproteinases types 1, 2, 3 and 9 in breast cancer.
title_fullStr Assay of matrix metalloproteinases types 1, 2, 3 and 9 in breast cancer.
title_full_unstemmed Assay of matrix metalloproteinases types 1, 2, 3 and 9 in breast cancer.
title_short Assay of matrix metalloproteinases types 1, 2, 3 and 9 in breast cancer.
title_sort assay of matrix metalloproteinases types 1, 2, 3 and 9 in breast cancer.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150110/
https://www.ncbi.nlm.nih.gov/pubmed/9528836
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