Cargando…

Expression of HGF/SF in mesothelioma cell lines and its effects on cell motility, proliferation and morphology.

The expression of hepatocyte growth factor/scatter factor (HGF/SF) was studied in 12 mesothelioma cell lines characterized by either an epithelioid or a fibroblast-like phenotype. Conditioned media from these lines were analysed by bioassay and ELISA, and HGF/SF was detected in three cell lines, all...

Descripción completa

Detalles Bibliográficos
Autores principales: Harvey, P., Warn, A., Dobbin, S., Arakaki, N., Daikuhara, Y., Jaurand, M. C., Warn, R. M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150119/
https://www.ncbi.nlm.nih.gov/pubmed/9569039
_version_ 1782144578116124672
author Harvey, P.
Warn, A.
Dobbin, S.
Arakaki, N.
Daikuhara, Y.
Jaurand, M. C.
Warn, R. M.
author_facet Harvey, P.
Warn, A.
Dobbin, S.
Arakaki, N.
Daikuhara, Y.
Jaurand, M. C.
Warn, R. M.
author_sort Harvey, P.
collection PubMed
description The expression of hepatocyte growth factor/scatter factor (HGF/SF) was studied in 12 mesothelioma cell lines characterized by either an epithelioid or a fibroblast-like phenotype. Conditioned media from these lines were analysed by bioassay and ELISA, and HGF/SF was detected in three cell lines, all with a fibroblast-like or mixed morphology. None of eight epithelioid cell lines expressed the factor. Thus, for these cell lines, the ability to secrete HGF/SF correlated with the cell phenotype. Following on from these observations, two cell lines, BR and BT, with a fibroblast-like and an epithelioid phenotype, respectively, were further investigated. Both cell lines expressed the Met receptor but only BR secreted HGF/SF. Both cell lines responded to exogenous HGF/SF treatment by a change of morphology but in different ways: BR became more elongated and bipolar, while BT formed more spread-out cell colonies. HGF/SF acted as a paracrine effector on the epithelioid BT cells and stimulated both cell-spreading and proliferation. Interestingly, BT cells spread but did not scatter in response to exogenous HGF/SF. In contrast BR cells showed only some stimulation of cell motility with HGF/SF and no increase in cell proliferation was observed. Because HGF/SF was previously found in the pleural effusion fluids of patients with malignant mesothelioma and in paraffin-embedded tumour tissues, it is concluded that HGF/SF may well stimulate the growth and spread of malignant mesothelioma in vivo by paracrine and/or autocrine mechanisms. IMAGES:
format Text
id pubmed-2150119
institution National Center for Biotechnology Information
language English
publishDate 1998
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-21501192009-09-10 Expression of HGF/SF in mesothelioma cell lines and its effects on cell motility, proliferation and morphology. Harvey, P. Warn, A. Dobbin, S. Arakaki, N. Daikuhara, Y. Jaurand, M. C. Warn, R. M. Br J Cancer Research Article The expression of hepatocyte growth factor/scatter factor (HGF/SF) was studied in 12 mesothelioma cell lines characterized by either an epithelioid or a fibroblast-like phenotype. Conditioned media from these lines were analysed by bioassay and ELISA, and HGF/SF was detected in three cell lines, all with a fibroblast-like or mixed morphology. None of eight epithelioid cell lines expressed the factor. Thus, for these cell lines, the ability to secrete HGF/SF correlated with the cell phenotype. Following on from these observations, two cell lines, BR and BT, with a fibroblast-like and an epithelioid phenotype, respectively, were further investigated. Both cell lines expressed the Met receptor but only BR secreted HGF/SF. Both cell lines responded to exogenous HGF/SF treatment by a change of morphology but in different ways: BR became more elongated and bipolar, while BT formed more spread-out cell colonies. HGF/SF acted as a paracrine effector on the epithelioid BT cells and stimulated both cell-spreading and proliferation. Interestingly, BT cells spread but did not scatter in response to exogenous HGF/SF. In contrast BR cells showed only some stimulation of cell motility with HGF/SF and no increase in cell proliferation was observed. Because HGF/SF was previously found in the pleural effusion fluids of patients with malignant mesothelioma and in paraffin-embedded tumour tissues, it is concluded that HGF/SF may well stimulate the growth and spread of malignant mesothelioma in vivo by paracrine and/or autocrine mechanisms. IMAGES: Nature Publishing Group 1998-04 /pmc/articles/PMC2150119/ /pubmed/9569039 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Harvey, P.
Warn, A.
Dobbin, S.
Arakaki, N.
Daikuhara, Y.
Jaurand, M. C.
Warn, R. M.
Expression of HGF/SF in mesothelioma cell lines and its effects on cell motility, proliferation and morphology.
title Expression of HGF/SF in mesothelioma cell lines and its effects on cell motility, proliferation and morphology.
title_full Expression of HGF/SF in mesothelioma cell lines and its effects on cell motility, proliferation and morphology.
title_fullStr Expression of HGF/SF in mesothelioma cell lines and its effects on cell motility, proliferation and morphology.
title_full_unstemmed Expression of HGF/SF in mesothelioma cell lines and its effects on cell motility, proliferation and morphology.
title_short Expression of HGF/SF in mesothelioma cell lines and its effects on cell motility, proliferation and morphology.
title_sort expression of hgf/sf in mesothelioma cell lines and its effects on cell motility, proliferation and morphology.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150119/
https://www.ncbi.nlm.nih.gov/pubmed/9569039
work_keys_str_mv AT harveyp expressionofhgfsfinmesotheliomacelllinesanditseffectsoncellmotilityproliferationandmorphology
AT warna expressionofhgfsfinmesotheliomacelllinesanditseffectsoncellmotilityproliferationandmorphology
AT dobbins expressionofhgfsfinmesotheliomacelllinesanditseffectsoncellmotilityproliferationandmorphology
AT arakakin expressionofhgfsfinmesotheliomacelllinesanditseffectsoncellmotilityproliferationandmorphology
AT daikuharay expressionofhgfsfinmesotheliomacelllinesanditseffectsoncellmotilityproliferationandmorphology
AT jaurandmc expressionofhgfsfinmesotheliomacelllinesanditseffectsoncellmotilityproliferationandmorphology
AT warnrm expressionofhgfsfinmesotheliomacelllinesanditseffectsoncellmotilityproliferationandmorphology