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Putative markers for the detection of breast carcinoma cells in blood.
The aim of this study was to investigate certain genes for their suitability as molecular markers for detection of breast carcinoma cells using the reverse transcriptase-polymerase chain reaction (RT-PCR). RNA was prepared from MCF-7 breast carcinoma cells and peripheral blood leucocytes of healthy...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
1998
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150149/ https://www.ncbi.nlm.nih.gov/pubmed/9579823 |
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author | Eltahir, E. M. Mallinson, D. S. Birnie, G. D. Hagan, C. George, W. D. Purushotham, A. D. |
author_facet | Eltahir, E. M. Mallinson, D. S. Birnie, G. D. Hagan, C. George, W. D. Purushotham, A. D. |
author_sort | Eltahir, E. M. |
collection | PubMed |
description | The aim of this study was to investigate certain genes for their suitability as molecular markers for detection of breast carcinoma cells using the reverse transcriptase-polymerase chain reaction (RT-PCR). RNA was prepared from MCF-7 breast carcinoma cells and peripheral blood leucocytes of healthy female volunteers. This RNA was screened for mRNA of MUC1, cytokeratin 19 (CK19) and CD44 (exons 8-11) by RT-PCR and the results validated by Southern blots. Variable degrees of expression of MUC1 and CD44 (exons 8-11) were detected in normal peripheral blood, rendering these genes non-specific for epithelial cells and therefore unsuitable for use as markers to detect breast carcinoma cells. Although CK19 mRNA was apparently specific, it was deemed unsuitable for use as a marker of breast cancer cells in light of its limited sensitivity. Furthermore, an attempt at using nested primers to increase sensitivity resulted in CK19 mRNA being detected after two amplification rounds in blood from healthy volunteers. IMAGES: |
format | Text |
id | pubmed-2150149 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1998 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-21501492009-09-10 Putative markers for the detection of breast carcinoma cells in blood. Eltahir, E. M. Mallinson, D. S. Birnie, G. D. Hagan, C. George, W. D. Purushotham, A. D. Br J Cancer Research Article The aim of this study was to investigate certain genes for their suitability as molecular markers for detection of breast carcinoma cells using the reverse transcriptase-polymerase chain reaction (RT-PCR). RNA was prepared from MCF-7 breast carcinoma cells and peripheral blood leucocytes of healthy female volunteers. This RNA was screened for mRNA of MUC1, cytokeratin 19 (CK19) and CD44 (exons 8-11) by RT-PCR and the results validated by Southern blots. Variable degrees of expression of MUC1 and CD44 (exons 8-11) were detected in normal peripheral blood, rendering these genes non-specific for epithelial cells and therefore unsuitable for use as markers to detect breast carcinoma cells. Although CK19 mRNA was apparently specific, it was deemed unsuitable for use as a marker of breast cancer cells in light of its limited sensitivity. Furthermore, an attempt at using nested primers to increase sensitivity resulted in CK19 mRNA being detected after two amplification rounds in blood from healthy volunteers. IMAGES: Nature Publishing Group 1998-04 /pmc/articles/PMC2150149/ /pubmed/9579823 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Eltahir, E. M. Mallinson, D. S. Birnie, G. D. Hagan, C. George, W. D. Purushotham, A. D. Putative markers for the detection of breast carcinoma cells in blood. |
title | Putative markers for the detection of breast carcinoma cells in blood. |
title_full | Putative markers for the detection of breast carcinoma cells in blood. |
title_fullStr | Putative markers for the detection of breast carcinoma cells in blood. |
title_full_unstemmed | Putative markers for the detection of breast carcinoma cells in blood. |
title_short | Putative markers for the detection of breast carcinoma cells in blood. |
title_sort | putative markers for the detection of breast carcinoma cells in blood. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150149/ https://www.ncbi.nlm.nih.gov/pubmed/9579823 |
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