Cargando…

Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells.

In situ hybridization on human colon tissue demonstrates that epidermal growth factor receptor (EGFR) mRNA expression is strongly increased during tumour progression. To obtain test systems to evaluate the relevance of growth factor action during carcinogenesis, primary cultures from human colorecta...

Descripción completa

Detalles Bibliográficos
Autores principales: Tong, W. M., Ellinger, A., Sheinin, Y., Cross, H. S.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150335/
https://www.ncbi.nlm.nih.gov/pubmed/9667648
_version_ 1782144602396950528
author Tong, W. M.
Ellinger, A.
Sheinin, Y.
Cross, H. S.
author_facet Tong, W. M.
Ellinger, A.
Sheinin, Y.
Cross, H. S.
author_sort Tong, W. M.
collection PubMed
description In situ hybridization on human colon tissue demonstrates that epidermal growth factor receptor (EGFR) mRNA expression is strongly increased during tumour progression. To obtain test systems to evaluate the relevance of growth factor action during carcinogenesis, primary cultures from human colorectal carcinomas were established. EGFR distribution was determined in 2 of the 27 primary cultures and was compared with that in well-defined subclones derived from the Caco-2 cell line, which has the unique property to differentiate spontaneously in vitro in a manner similar to normal enterocytes. The primary carcinoma-derived cells had up to three-fold higher total EGFR levels than the Caco-2 subclones and a basal mitotic rate at least fourfold higher. The EGFR affinity constant is 0.26 nmol l(-1), which is similar to that reported in Caco-2 cells. The proliferation rate of Caco-2 cells is mainly induced by EGF from the basolateral cell surface where the majority of receptors are located, whereas primary cultures are strongly stimulated from the apical side also. This corresponds to a three- to fivefold higher level of EGFR at the apical cell surface. This redistribution of EGFR to apical plasma membranes in advanced colon carcinoma cells suggests that autocrine growth factors in the colon lumen may play a significant role during tumour progression. IMAGES:
format Text
id pubmed-2150335
institution National Center for Biotechnology Information
language English
publishDate 1998
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-21503352009-09-10 Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells. Tong, W. M. Ellinger, A. Sheinin, Y. Cross, H. S. Br J Cancer Research Article In situ hybridization on human colon tissue demonstrates that epidermal growth factor receptor (EGFR) mRNA expression is strongly increased during tumour progression. To obtain test systems to evaluate the relevance of growth factor action during carcinogenesis, primary cultures from human colorectal carcinomas were established. EGFR distribution was determined in 2 of the 27 primary cultures and was compared with that in well-defined subclones derived from the Caco-2 cell line, which has the unique property to differentiate spontaneously in vitro in a manner similar to normal enterocytes. The primary carcinoma-derived cells had up to three-fold higher total EGFR levels than the Caco-2 subclones and a basal mitotic rate at least fourfold higher. The EGFR affinity constant is 0.26 nmol l(-1), which is similar to that reported in Caco-2 cells. The proliferation rate of Caco-2 cells is mainly induced by EGF from the basolateral cell surface where the majority of receptors are located, whereas primary cultures are strongly stimulated from the apical side also. This corresponds to a three- to fivefold higher level of EGFR at the apical cell surface. This redistribution of EGFR to apical plasma membranes in advanced colon carcinoma cells suggests that autocrine growth factors in the colon lumen may play a significant role during tumour progression. IMAGES: Nature Publishing Group 1998-06 /pmc/articles/PMC2150335/ /pubmed/9667648 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Tong, W. M.
Ellinger, A.
Sheinin, Y.
Cross, H. S.
Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells.
title Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells.
title_full Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells.
title_fullStr Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells.
title_full_unstemmed Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells.
title_short Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells.
title_sort epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150335/
https://www.ncbi.nlm.nih.gov/pubmed/9667648
work_keys_str_mv AT tongwm epidermalgrowthfactorreceptorexpressioninprimaryculturedhumancolorectalcarcinomacells
AT ellingera epidermalgrowthfactorreceptorexpressioninprimaryculturedhumancolorectalcarcinomacells
AT sheininy epidermalgrowthfactorreceptorexpressioninprimaryculturedhumancolorectalcarcinomacells
AT crosshs epidermalgrowthfactorreceptorexpressioninprimaryculturedhumancolorectalcarcinomacells