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Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells.
In situ hybridization on human colon tissue demonstrates that epidermal growth factor receptor (EGFR) mRNA expression is strongly increased during tumour progression. To obtain test systems to evaluate the relevance of growth factor action during carcinogenesis, primary cultures from human colorecta...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1998
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150335/ https://www.ncbi.nlm.nih.gov/pubmed/9667648 |
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author | Tong, W. M. Ellinger, A. Sheinin, Y. Cross, H. S. |
author_facet | Tong, W. M. Ellinger, A. Sheinin, Y. Cross, H. S. |
author_sort | Tong, W. M. |
collection | PubMed |
description | In situ hybridization on human colon tissue demonstrates that epidermal growth factor receptor (EGFR) mRNA expression is strongly increased during tumour progression. To obtain test systems to evaluate the relevance of growth factor action during carcinogenesis, primary cultures from human colorectal carcinomas were established. EGFR distribution was determined in 2 of the 27 primary cultures and was compared with that in well-defined subclones derived from the Caco-2 cell line, which has the unique property to differentiate spontaneously in vitro in a manner similar to normal enterocytes. The primary carcinoma-derived cells had up to three-fold higher total EGFR levels than the Caco-2 subclones and a basal mitotic rate at least fourfold higher. The EGFR affinity constant is 0.26 nmol l(-1), which is similar to that reported in Caco-2 cells. The proliferation rate of Caco-2 cells is mainly induced by EGF from the basolateral cell surface where the majority of receptors are located, whereas primary cultures are strongly stimulated from the apical side also. This corresponds to a three- to fivefold higher level of EGFR at the apical cell surface. This redistribution of EGFR to apical plasma membranes in advanced colon carcinoma cells suggests that autocrine growth factors in the colon lumen may play a significant role during tumour progression. IMAGES: |
format | Text |
id | pubmed-2150335 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1998 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-21503352009-09-10 Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells. Tong, W. M. Ellinger, A. Sheinin, Y. Cross, H. S. Br J Cancer Research Article In situ hybridization on human colon tissue demonstrates that epidermal growth factor receptor (EGFR) mRNA expression is strongly increased during tumour progression. To obtain test systems to evaluate the relevance of growth factor action during carcinogenesis, primary cultures from human colorectal carcinomas were established. EGFR distribution was determined in 2 of the 27 primary cultures and was compared with that in well-defined subclones derived from the Caco-2 cell line, which has the unique property to differentiate spontaneously in vitro in a manner similar to normal enterocytes. The primary carcinoma-derived cells had up to three-fold higher total EGFR levels than the Caco-2 subclones and a basal mitotic rate at least fourfold higher. The EGFR affinity constant is 0.26 nmol l(-1), which is similar to that reported in Caco-2 cells. The proliferation rate of Caco-2 cells is mainly induced by EGF from the basolateral cell surface where the majority of receptors are located, whereas primary cultures are strongly stimulated from the apical side also. This corresponds to a three- to fivefold higher level of EGFR at the apical cell surface. This redistribution of EGFR to apical plasma membranes in advanced colon carcinoma cells suggests that autocrine growth factors in the colon lumen may play a significant role during tumour progression. IMAGES: Nature Publishing Group 1998-06 /pmc/articles/PMC2150335/ /pubmed/9667648 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Tong, W. M. Ellinger, A. Sheinin, Y. Cross, H. S. Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells. |
title | Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells. |
title_full | Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells. |
title_fullStr | Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells. |
title_full_unstemmed | Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells. |
title_short | Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells. |
title_sort | epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150335/ https://www.ncbi.nlm.nih.gov/pubmed/9667648 |
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