Mutations of the Apc gene in experimental colorectal carcinogenesis induced by azoxymethane in F344 rats.

We investigated in the rat the role of the Apc gene, which is mutated in familial adenomatous polyposis and sporadic colon cancer in the process leading from normal colonic mucosa to aberrant crypt foci (ACF) and finally to adenomas and adenocarcinomas. We analysed mutations in exon 15 of the rat Ap...

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Autores principales: De Filippo, C., Caderni, G., Bazzicalupo, M., Briani, C., Giannini, A., Fazi, M., Dolara, P.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1998
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150417/
https://www.ncbi.nlm.nih.gov/pubmed/9649126
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author De Filippo, C.
Caderni, G.
Bazzicalupo, M.
Briani, C.
Giannini, A.
Fazi, M.
Dolara, P.
author_facet De Filippo, C.
Caderni, G.
Bazzicalupo, M.
Briani, C.
Giannini, A.
Fazi, M.
Dolara, P.
author_sort De Filippo, C.
collection PubMed
description We investigated in the rat the role of the Apc gene, which is mutated in familial adenomatous polyposis and sporadic colon cancer in the process leading from normal colonic mucosa to aberrant crypt foci (ACF) and finally to adenomas and adenocarcinomas. We analysed mutations in exon 15 of the rat Apc gene using in vitro synthesized protein assay in 66 ACF and in 28 colon tumours induced by azoxymethane. No Apc mutations were found in ACF, whereas five mutations were found in the tumours. The data suggest that mutations of the Apc gene are associated with the transition from ACF to adenoma and adenocarcinoma and not from normal mucosa to ACF.
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spelling pubmed-21504172009-09-10 Mutations of the Apc gene in experimental colorectal carcinogenesis induced by azoxymethane in F344 rats. De Filippo, C. Caderni, G. Bazzicalupo, M. Briani, C. Giannini, A. Fazi, M. Dolara, P. Br J Cancer Research Article We investigated in the rat the role of the Apc gene, which is mutated in familial adenomatous polyposis and sporadic colon cancer in the process leading from normal colonic mucosa to aberrant crypt foci (ACF) and finally to adenomas and adenocarcinomas. We analysed mutations in exon 15 of the rat Apc gene using in vitro synthesized protein assay in 66 ACF and in 28 colon tumours induced by azoxymethane. No Apc mutations were found in ACF, whereas five mutations were found in the tumours. The data suggest that mutations of the Apc gene are associated with the transition from ACF to adenoma and adenocarcinoma and not from normal mucosa to ACF. Nature Publishing Group 1998-06 /pmc/articles/PMC2150417/ /pubmed/9649126 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
De Filippo, C.
Caderni, G.
Bazzicalupo, M.
Briani, C.
Giannini, A.
Fazi, M.
Dolara, P.
Mutations of the Apc gene in experimental colorectal carcinogenesis induced by azoxymethane in F344 rats.
title Mutations of the Apc gene in experimental colorectal carcinogenesis induced by azoxymethane in F344 rats.
title_full Mutations of the Apc gene in experimental colorectal carcinogenesis induced by azoxymethane in F344 rats.
title_fullStr Mutations of the Apc gene in experimental colorectal carcinogenesis induced by azoxymethane in F344 rats.
title_full_unstemmed Mutations of the Apc gene in experimental colorectal carcinogenesis induced by azoxymethane in F344 rats.
title_short Mutations of the Apc gene in experimental colorectal carcinogenesis induced by azoxymethane in F344 rats.
title_sort mutations of the apc gene in experimental colorectal carcinogenesis induced by azoxymethane in f344 rats.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150417/
https://www.ncbi.nlm.nih.gov/pubmed/9649126
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