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Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus

The Golgi apparatus is a highly complex organelle comprised of a stack of cisternal membranes on the secretory pathway from the ER to the cell surface. This structure is maintained by an exoskeleton or Golgi matrix constructed from a family of coiled-coil proteins, the golgins, and other peripheral...

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Detalles Bibliográficos
Autores principales: Barr, Francis A., Preisinger, Christian, Kopajtich, Robert, Körner, Roman
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150891/
https://www.ncbi.nlm.nih.gov/pubmed/11739402
http://dx.doi.org/10.1083/jcb.200108102
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author Barr, Francis A.
Preisinger, Christian
Kopajtich, Robert
Körner, Roman
author_facet Barr, Francis A.
Preisinger, Christian
Kopajtich, Robert
Körner, Roman
author_sort Barr, Francis A.
collection PubMed
description The Golgi apparatus is a highly complex organelle comprised of a stack of cisternal membranes on the secretory pathway from the ER to the cell surface. This structure is maintained by an exoskeleton or Golgi matrix constructed from a family of coiled-coil proteins, the golgins, and other peripheral membrane components such as GRASP55 and GRASP65. Here we find that TMP21, p24a, and gp25L, members of the p24 cargo receptor family, are present in complexes with GRASP55 and GRASP65 in vivo. GRASPs interact directly with the cytoplasmic domains of specific p24 cargo receptors depending on their oligomeric state, and mutation of the GRASP binding site in the cytoplasmic tail of one of these, p24a, results in it being transported to the cell surface. These results suggest that one function of the Golgi matrix is to aid efficient retention or sequestration of p24 cargo receptors and other membrane proteins in the Golgi apparatus.
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spelling pubmed-21508912008-05-01 Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus Barr, Francis A. Preisinger, Christian Kopajtich, Robert Körner, Roman J Cell Biol Report The Golgi apparatus is a highly complex organelle comprised of a stack of cisternal membranes on the secretory pathway from the ER to the cell surface. This structure is maintained by an exoskeleton or Golgi matrix constructed from a family of coiled-coil proteins, the golgins, and other peripheral membrane components such as GRASP55 and GRASP65. Here we find that TMP21, p24a, and gp25L, members of the p24 cargo receptor family, are present in complexes with GRASP55 and GRASP65 in vivo. GRASPs interact directly with the cytoplasmic domains of specific p24 cargo receptors depending on their oligomeric state, and mutation of the GRASP binding site in the cytoplasmic tail of one of these, p24a, results in it being transported to the cell surface. These results suggest that one function of the Golgi matrix is to aid efficient retention or sequestration of p24 cargo receptors and other membrane proteins in the Golgi apparatus. The Rockefeller University Press 2001-12-10 /pmc/articles/PMC2150891/ /pubmed/11739402 http://dx.doi.org/10.1083/jcb.200108102 Text en Copyright © 2001, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Report
Barr, Francis A.
Preisinger, Christian
Kopajtich, Robert
Körner, Roman
Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus
title Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus
title_full Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus
title_fullStr Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus
title_full_unstemmed Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus
title_short Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus
title_sort golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the golgi apparatus
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150891/
https://www.ncbi.nlm.nih.gov/pubmed/11739402
http://dx.doi.org/10.1083/jcb.200108102
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