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A t-SNARE of the endocytic pathway must be activated for fusion
The t-SNARE in a late Golgi compartment (Tlg2p) syntaxin is required for endocytosis and localization of cycling proteins to the late Golgi compartment in yeast. We show here that Tlg2p assembles with two light chains, Tlg1p and Vti1p, to form a functional t-SNARE that mediates fusion, specifically...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2001
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150898/ https://www.ncbi.nlm.nih.gov/pubmed/11739407 http://dx.doi.org/10.1083/jcb.200104092 |
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author | Paumet, Fabienne Brügger, Britta Parlati, Francesco McNew, James A. Söllner, Thomas H. Rothman, James E. |
author_facet | Paumet, Fabienne Brügger, Britta Parlati, Francesco McNew, James A. Söllner, Thomas H. Rothman, James E. |
author_sort | Paumet, Fabienne |
collection | PubMed |
description | The t-SNARE in a late Golgi compartment (Tlg2p) syntaxin is required for endocytosis and localization of cycling proteins to the late Golgi compartment in yeast. We show here that Tlg2p assembles with two light chains, Tlg1p and Vti1p, to form a functional t-SNARE that mediates fusion, specifically with the v-SNAREs Snc1p and Snc2p. In vitro, this t-SNARE is inert, locked in a nonfunctional state, unless it is activated for fusion. Activation can be mediated by a peptide derived from the v-SNARE, which likely bypasses additional regulatory proteins in the cell. Locking t-SNAREs creates the potential for spatial and temporal regulation of fusion by signaling processes that unleash their fusion capacity. |
format | Text |
id | pubmed-2150898 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21508982008-05-01 A t-SNARE of the endocytic pathway must be activated for fusion Paumet, Fabienne Brügger, Britta Parlati, Francesco McNew, James A. Söllner, Thomas H. Rothman, James E. J Cell Biol Article The t-SNARE in a late Golgi compartment (Tlg2p) syntaxin is required for endocytosis and localization of cycling proteins to the late Golgi compartment in yeast. We show here that Tlg2p assembles with two light chains, Tlg1p and Vti1p, to form a functional t-SNARE that mediates fusion, specifically with the v-SNAREs Snc1p and Snc2p. In vitro, this t-SNARE is inert, locked in a nonfunctional state, unless it is activated for fusion. Activation can be mediated by a peptide derived from the v-SNARE, which likely bypasses additional regulatory proteins in the cell. Locking t-SNAREs creates the potential for spatial and temporal regulation of fusion by signaling processes that unleash their fusion capacity. The Rockefeller University Press 2001-12-10 /pmc/articles/PMC2150898/ /pubmed/11739407 http://dx.doi.org/10.1083/jcb.200104092 Text en Copyright © 2001, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Paumet, Fabienne Brügger, Britta Parlati, Francesco McNew, James A. Söllner, Thomas H. Rothman, James E. A t-SNARE of the endocytic pathway must be activated for fusion |
title | A t-SNARE of the endocytic pathway must be activated for fusion |
title_full | A t-SNARE of the endocytic pathway must be activated for fusion |
title_fullStr | A t-SNARE of the endocytic pathway must be activated for fusion |
title_full_unstemmed | A t-SNARE of the endocytic pathway must be activated for fusion |
title_short | A t-SNARE of the endocytic pathway must be activated for fusion |
title_sort | t-snare of the endocytic pathway must be activated for fusion |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150898/ https://www.ncbi.nlm.nih.gov/pubmed/11739407 http://dx.doi.org/10.1083/jcb.200104092 |
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