A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic

Membrane traffic between the endoplasmic reticulum (ER) and Golgi apparatus and through the Golgi apparatus is a highly regulated process controlled by members of the rab GTPase family. The GTP form of rab1 regulates ER to Golgi transport by interaction with the vesicle tethering factor p115 and the...

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Autores principales: Short, Benjamin, Preisinger, Christian, Körner, Roman, Kopajtich, Robert, Byron, Olwyn, Barr, Francis A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2001
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150909/
https://www.ncbi.nlm.nih.gov/pubmed/11739401
http://dx.doi.org/10.1083/jcb.200108079
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author Short, Benjamin
Preisinger, Christian
Körner, Roman
Kopajtich, Robert
Byron, Olwyn
Barr, Francis A.
author_facet Short, Benjamin
Preisinger, Christian
Körner, Roman
Kopajtich, Robert
Byron, Olwyn
Barr, Francis A.
author_sort Short, Benjamin
collection PubMed
description Membrane traffic between the endoplasmic reticulum (ER) and Golgi apparatus and through the Golgi apparatus is a highly regulated process controlled by members of the rab GTPase family. The GTP form of rab1 regulates ER to Golgi transport by interaction with the vesicle tethering factor p115 and the cis-Golgi matrix protein GM130, also part of a complex with GRASP65 important for the organization of cis-Golgi cisternae. Here, we find that a novel coiled-coil protein golgin-45 interacts with the medial-Golgi matrix protein GRASP55 and the GTP form of rab2 but not other Golgi rab proteins. Depletion of golgin-45 disrupts the Golgi apparatus and causes a block in secretory protein transport. These results demonstrate that GRASP55 and golgin-45 form a rab2 effector complex on medial-Golgi essential for normal protein transport and Golgi structure.
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spelling pubmed-21509092008-05-01 A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic Short, Benjamin Preisinger, Christian Körner, Roman Kopajtich, Robert Byron, Olwyn Barr, Francis A. J Cell Biol Report Membrane traffic between the endoplasmic reticulum (ER) and Golgi apparatus and through the Golgi apparatus is a highly regulated process controlled by members of the rab GTPase family. The GTP form of rab1 regulates ER to Golgi transport by interaction with the vesicle tethering factor p115 and the cis-Golgi matrix protein GM130, also part of a complex with GRASP65 important for the organization of cis-Golgi cisternae. Here, we find that a novel coiled-coil protein golgin-45 interacts with the medial-Golgi matrix protein GRASP55 and the GTP form of rab2 but not other Golgi rab proteins. Depletion of golgin-45 disrupts the Golgi apparatus and causes a block in secretory protein transport. These results demonstrate that GRASP55 and golgin-45 form a rab2 effector complex on medial-Golgi essential for normal protein transport and Golgi structure. The Rockefeller University Press 2001-12-10 /pmc/articles/PMC2150909/ /pubmed/11739401 http://dx.doi.org/10.1083/jcb.200108079 Text en Copyright © 2001, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Report
Short, Benjamin
Preisinger, Christian
Körner, Roman
Kopajtich, Robert
Byron, Olwyn
Barr, Francis A.
A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic
title A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic
title_full A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic
title_fullStr A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic
title_full_unstemmed A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic
title_short A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic
title_sort grasp55-rab2 effector complex linking golgi structure to membrane traffic
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150909/
https://www.ncbi.nlm.nih.gov/pubmed/11739401
http://dx.doi.org/10.1083/jcb.200108079
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