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Parvovirus B19 infection in Tunisian patients with sickle-cell anemia and acute erythroblastopenia

BACKGROUND: Human parvovirus B19 is the etiologic agent of erythema infectiosum in children. It is also associated with other clinical manifestations in different target groups. Patients with chronic hemolytic anemia are at high risk of developing acute erythroblastopenia following infection by the...

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Autores principales: Regaya, Faouzi, Oussaief, Lassad, Bejaoui, Mohamed, Karoui, Mongi, Zili, Mohamed, Khelifa, Ridha
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151067/
https://www.ncbi.nlm.nih.gov/pubmed/17961236
http://dx.doi.org/10.1186/1471-2334-7-123
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author Regaya, Faouzi
Oussaief, Lassad
Bejaoui, Mohamed
Karoui, Mongi
Zili, Mohamed
Khelifa, Ridha
author_facet Regaya, Faouzi
Oussaief, Lassad
Bejaoui, Mohamed
Karoui, Mongi
Zili, Mohamed
Khelifa, Ridha
author_sort Regaya, Faouzi
collection PubMed
description BACKGROUND: Human parvovirus B19 is the etiologic agent of erythema infectiosum in children. It is also associated with other clinical manifestations in different target groups. Patients with chronic hemolytic anemia are at high risk of developing acute erythroblastopenia following infection by the virus. They usually become highly viremic and pose an increased risk of virus transmission. Close monitoring of such high risk groups is required for epidemiologic surveillance and disease prevention activities. Here we report a molecular epidemiological study on B19 virus infection in Tunisian patients with chronic hemolytic anemia. METHODS: This study was conducted on 92 young chronic hemolytic anemia patients who attended the same ward at the National Bone Marrow Transplantation Center of Tunis and 46 controls from a different hospital. Screening for IgM and IgG anti-B19 antibodies was performed using commercially available enzyme immunoassays and B19 DNA was detected by nested PCR in the overlapping VP1/VP2 region. DNA was sequenced using dideoxy-terminator cycle sequencing technology. RESULTS: Anti-parvovirus B19 IgG antibodies were detected in 26 of 46 sickle-cell anemia patients, 18 of 46 β-thalassemia and 7 of 46 controls. Anti-parvovirus B19 IgM antibodies were detected only in 4 of the sickle-cell anemia patients: two siblings and two unrelated who presented with acute erythroblastopenia at the time of blood collection for this study and had no history of past transfusion. B19 DNA was detected only in sera of these four patients and the corresponding 288 bp nested DNA amplicons were sequenced. The sequences obtained were all identical and phylogenetic analysis showed that they belonged to a new B19 virus strain of Genotype1. CONCLUSION: A new parvovirus B19 strain of genotype1 was detected in four Tunisian patients with sickle-cell anemia. Virus transmission appeared to be nosocomial and resulted in acute erythroblastopenia in the four patients. The possibility of independent transmission of this B19 variant to the patients is unlikely in light of the present epidemiological data. However this possibility cannot be ruled out because of the low genetic variability of the virus.
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spelling pubmed-21510672007-12-21 Parvovirus B19 infection in Tunisian patients with sickle-cell anemia and acute erythroblastopenia Regaya, Faouzi Oussaief, Lassad Bejaoui, Mohamed Karoui, Mongi Zili, Mohamed Khelifa, Ridha BMC Infect Dis Research Article BACKGROUND: Human parvovirus B19 is the etiologic agent of erythema infectiosum in children. It is also associated with other clinical manifestations in different target groups. Patients with chronic hemolytic anemia are at high risk of developing acute erythroblastopenia following infection by the virus. They usually become highly viremic and pose an increased risk of virus transmission. Close monitoring of such high risk groups is required for epidemiologic surveillance and disease prevention activities. Here we report a molecular epidemiological study on B19 virus infection in Tunisian patients with chronic hemolytic anemia. METHODS: This study was conducted on 92 young chronic hemolytic anemia patients who attended the same ward at the National Bone Marrow Transplantation Center of Tunis and 46 controls from a different hospital. Screening for IgM and IgG anti-B19 antibodies was performed using commercially available enzyme immunoassays and B19 DNA was detected by nested PCR in the overlapping VP1/VP2 region. DNA was sequenced using dideoxy-terminator cycle sequencing technology. RESULTS: Anti-parvovirus B19 IgG antibodies were detected in 26 of 46 sickle-cell anemia patients, 18 of 46 β-thalassemia and 7 of 46 controls. Anti-parvovirus B19 IgM antibodies were detected only in 4 of the sickle-cell anemia patients: two siblings and two unrelated who presented with acute erythroblastopenia at the time of blood collection for this study and had no history of past transfusion. B19 DNA was detected only in sera of these four patients and the corresponding 288 bp nested DNA amplicons were sequenced. The sequences obtained were all identical and phylogenetic analysis showed that they belonged to a new B19 virus strain of Genotype1. CONCLUSION: A new parvovirus B19 strain of genotype1 was detected in four Tunisian patients with sickle-cell anemia. Virus transmission appeared to be nosocomial and resulted in acute erythroblastopenia in the four patients. The possibility of independent transmission of this B19 variant to the patients is unlikely in light of the present epidemiological data. However this possibility cannot be ruled out because of the low genetic variability of the virus. BioMed Central 2007-10-25 /pmc/articles/PMC2151067/ /pubmed/17961236 http://dx.doi.org/10.1186/1471-2334-7-123 Text en Copyright © 2007 Regaya et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Regaya, Faouzi
Oussaief, Lassad
Bejaoui, Mohamed
Karoui, Mongi
Zili, Mohamed
Khelifa, Ridha
Parvovirus B19 infection in Tunisian patients with sickle-cell anemia and acute erythroblastopenia
title Parvovirus B19 infection in Tunisian patients with sickle-cell anemia and acute erythroblastopenia
title_full Parvovirus B19 infection in Tunisian patients with sickle-cell anemia and acute erythroblastopenia
title_fullStr Parvovirus B19 infection in Tunisian patients with sickle-cell anemia and acute erythroblastopenia
title_full_unstemmed Parvovirus B19 infection in Tunisian patients with sickle-cell anemia and acute erythroblastopenia
title_short Parvovirus B19 infection in Tunisian patients with sickle-cell anemia and acute erythroblastopenia
title_sort parvovirus b19 infection in tunisian patients with sickle-cell anemia and acute erythroblastopenia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151067/
https://www.ncbi.nlm.nih.gov/pubmed/17961236
http://dx.doi.org/10.1186/1471-2334-7-123
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