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Kinesin-II Is Required for Axonal Transport of Choline Acetyltransferase in Drosophila

KLP64D and KLP68D are members of the kinesin-II family of proteins in Drosophila. Immunostaining for KLP68D and ribonucleic acid in situ hybridization for KLP64D demonstrated their preferential expression in cholinergic neurons. KLP68D was also found to accumulate in cholinergic neurons in axonal ob...

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Detalles Bibliográficos
Autores principales: Ray, Krishanu, Perez, Sharon E., Yang, Zhaohuai, Xu, Jenny, Ritchings, Bruce W., Steller, Hermann, Goldstein, Lawrence S.B.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151187/
https://www.ncbi.nlm.nih.gov/pubmed/10545496
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author Ray, Krishanu
Perez, Sharon E.
Yang, Zhaohuai
Xu, Jenny
Ritchings, Bruce W.
Steller, Hermann
Goldstein, Lawrence S.B.
author_facet Ray, Krishanu
Perez, Sharon E.
Yang, Zhaohuai
Xu, Jenny
Ritchings, Bruce W.
Steller, Hermann
Goldstein, Lawrence S.B.
author_sort Ray, Krishanu
collection PubMed
description KLP64D and KLP68D are members of the kinesin-II family of proteins in Drosophila. Immunostaining for KLP68D and ribonucleic acid in situ hybridization for KLP64D demonstrated their preferential expression in cholinergic neurons. KLP68D was also found to accumulate in cholinergic neurons in axonal obstructions caused by the loss of kinesin light chain. Mutations in the KLP64D gene cause uncoordinated sluggish movement and death, and reduce transport of choline acetyltransferase from cell bodies to the synapse. The inviability of KLP64D mutations can be rescued by expression of mammalian KIF3A. Together, these data suggest that kinesin-II is required for the axonal transport of a soluble enzyme, choline acetyltransferase, in a specific subset of neurons in Drosophila. Furthermore, the data lead to the conclusion that the cargo transport requirements of different classes of neurons may lead to upregulation of specific pathways of axonal transport.
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spelling pubmed-21511872008-05-01 Kinesin-II Is Required for Axonal Transport of Choline Acetyltransferase in Drosophila Ray, Krishanu Perez, Sharon E. Yang, Zhaohuai Xu, Jenny Ritchings, Bruce W. Steller, Hermann Goldstein, Lawrence S.B. J Cell Biol Original Article KLP64D and KLP68D are members of the kinesin-II family of proteins in Drosophila. Immunostaining for KLP68D and ribonucleic acid in situ hybridization for KLP64D demonstrated their preferential expression in cholinergic neurons. KLP68D was also found to accumulate in cholinergic neurons in axonal obstructions caused by the loss of kinesin light chain. Mutations in the KLP64D gene cause uncoordinated sluggish movement and death, and reduce transport of choline acetyltransferase from cell bodies to the synapse. The inviability of KLP64D mutations can be rescued by expression of mammalian KIF3A. Together, these data suggest that kinesin-II is required for the axonal transport of a soluble enzyme, choline acetyltransferase, in a specific subset of neurons in Drosophila. Furthermore, the data lead to the conclusion that the cargo transport requirements of different classes of neurons may lead to upregulation of specific pathways of axonal transport. The Rockefeller University Press 1999-11-01 /pmc/articles/PMC2151187/ /pubmed/10545496 Text en © 1999 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Ray, Krishanu
Perez, Sharon E.
Yang, Zhaohuai
Xu, Jenny
Ritchings, Bruce W.
Steller, Hermann
Goldstein, Lawrence S.B.
Kinesin-II Is Required for Axonal Transport of Choline Acetyltransferase in Drosophila
title Kinesin-II Is Required for Axonal Transport of Choline Acetyltransferase in Drosophila
title_full Kinesin-II Is Required for Axonal Transport of Choline Acetyltransferase in Drosophila
title_fullStr Kinesin-II Is Required for Axonal Transport of Choline Acetyltransferase in Drosophila
title_full_unstemmed Kinesin-II Is Required for Axonal Transport of Choline Acetyltransferase in Drosophila
title_short Kinesin-II Is Required for Axonal Transport of Choline Acetyltransferase in Drosophila
title_sort kinesin-ii is required for axonal transport of choline acetyltransferase in drosophila
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151187/
https://www.ncbi.nlm.nih.gov/pubmed/10545496
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