Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia.

The human melanoma cell lines MM96L, A2058 and HT144 were examined for sensitivity to ionizing radiation and UVB radiation. HT144 demonstrated a significant increase in sensitivity to ionizing and UVB radiation compared with the MM96L and A2058 cells. Sensitivity to both agents was associated with s...

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Detalles Bibliográficos
Autores principales: Ramsay, J., Birrell, G., Baumann, K., Bodero, A., Parsons, P., Lavin, M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1998
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151277/
https://www.ncbi.nlm.nih.gov/pubmed/9459139
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author Ramsay, J.
Birrell, G.
Baumann, K.
Bodero, A.
Parsons, P.
Lavin, M.
author_facet Ramsay, J.
Birrell, G.
Baumann, K.
Bodero, A.
Parsons, P.
Lavin, M.
author_sort Ramsay, J.
collection PubMed
description The human melanoma cell lines MM96L, A2058 and HT144 were examined for sensitivity to ionizing radiation and UVB radiation. HT144 demonstrated a significant increase in sensitivity to ionizing and UVB radiation compared with the MM96L and A2058 cells. Sensitivity to both agents was associated with susceptibility to apoptosis. Using a protein truncation assay, a mutation for the gene for ataxia telangiectasia (ATM) was identified in HT144 cells. This was confirmed to be a homozygous mutation by subsequent sequencing of the abnormal region. Protein truncation assay of the other two cell lines showed no abnormality. The results suggest that somatic mutation of the A-T gene may be important in determining tumour radiosensitivity. IMAGES:
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spelling pubmed-21512772009-09-10 Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia. Ramsay, J. Birrell, G. Baumann, K. Bodero, A. Parsons, P. Lavin, M. Br J Cancer Research Article The human melanoma cell lines MM96L, A2058 and HT144 were examined for sensitivity to ionizing radiation and UVB radiation. HT144 demonstrated a significant increase in sensitivity to ionizing and UVB radiation compared with the MM96L and A2058 cells. Sensitivity to both agents was associated with susceptibility to apoptosis. Using a protein truncation assay, a mutation for the gene for ataxia telangiectasia (ATM) was identified in HT144 cells. This was confirmed to be a homozygous mutation by subsequent sequencing of the abnormal region. Protein truncation assay of the other two cell lines showed no abnormality. The results suggest that somatic mutation of the A-T gene may be important in determining tumour radiosensitivity. IMAGES: Nature Publishing Group 1998 /pmc/articles/PMC2151277/ /pubmed/9459139 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Ramsay, J.
Birrell, G.
Baumann, K.
Bodero, A.
Parsons, P.
Lavin, M.
Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia.
title Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia.
title_full Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia.
title_fullStr Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia.
title_full_unstemmed Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia.
title_short Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia.
title_sort radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151277/
https://www.ncbi.nlm.nih.gov/pubmed/9459139
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