Voltage Clamp Fluorometric Measurements on a Type II Na(+)-coupled P(i) Cotransporter: Shedding Light on Substrate Binding Order

Voltage clamp fluorometry (VCF) combines conventional two-electrode voltage clamp with fluorescence measurements to detect protein conformational changes, as sensed by a fluorophore covalently attached to the protein. We have applied VCF to a type IIb Na(+)-coupled phosphate cotransporter (NaPi-IIb)...

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Detalles Bibliográficos
Autores principales: Virkki, Leila V., Murer, Heini, Forster, Ian C.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151518/
https://www.ncbi.nlm.nih.gov/pubmed/16636203
http://dx.doi.org/10.1085/jgp.200609496

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151518/
https://www.ncbi.nlm.nih.gov/pubmed/16636203
http://dx.doi.org/10.1085/jgp.200609496

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