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Voltage Clamp Fluorometric Measurements on a Type II Na(+)-coupled P(i) Cotransporter: Shedding Light on Substrate Binding Order
Voltage clamp fluorometry (VCF) combines conventional two-electrode voltage clamp with fluorescence measurements to detect protein conformational changes, as sensed by a fluorophore covalently attached to the protein. We have applied VCF to a type IIb Na(+)-coupled phosphate cotransporter (NaPi-IIb)...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
2006
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151518/ https://www.ncbi.nlm.nih.gov/pubmed/16636203 http://dx.doi.org/10.1085/jgp.200609496 |