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Visual Cycle: Dependence of Retinol Production and Removal on Photoproduct Decay and Cell Morphology
The visual cycle is a chain of biochemical reactions that regenerate visual pigment following exposure to light. Initial steps, the liberation of all-trans retinal and its reduction to all-trans retinol by retinol dehydrogenase (RDH), take place in photoreceptors. We performed comparative microspect...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151530/ https://www.ncbi.nlm.nih.gov/pubmed/16847097 http://dx.doi.org/10.1085/jgp.200609557 |
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author | Ala-Laurila, Petri Kolesnikov, Alexander V. Crouch, Rosalie K. Tsina, Efthymia Shukolyukov, Sergey A. Govardovskii, Victor I. Koutalos, Yiannis Wiggert, Barbara Estevez, Maureen E. Cornwall, M. Carter |
author_facet | Ala-Laurila, Petri Kolesnikov, Alexander V. Crouch, Rosalie K. Tsina, Efthymia Shukolyukov, Sergey A. Govardovskii, Victor I. Koutalos, Yiannis Wiggert, Barbara Estevez, Maureen E. Cornwall, M. Carter |
author_sort | Ala-Laurila, Petri |
collection | PubMed |
description | The visual cycle is a chain of biochemical reactions that regenerate visual pigment following exposure to light. Initial steps, the liberation of all-trans retinal and its reduction to all-trans retinol by retinol dehydrogenase (RDH), take place in photoreceptors. We performed comparative microspectrophotometric and microfluorometric measurements on a variety of rod and cone photoreceptors isolated from salamander retinae to correlate the rates of photoproduct decay and retinol production. Metapigment decay rate was spatially uniform within outer segments and 50–70 times faster in the cells that contained cone-type pigment (SWS2 and M/LWS) compared to cells with rod-type pigment (RH1). Retinol production rate was strongly position dependent, fastest at the base of outer segments. Retinol production rate was 10–40 times faster in cones with cone pigments (SWS2 and M/LWS) than in the basal OS of rods containing rod pigment (RH1). Production rate was approximately five times faster in rods containing cone pigment (SWS2) than the rate in basal OS of rods containing the rod pigment (RH1). We show that retinol production is defined either by metapigment decay rate or RDH reaction rate, depending on cell type or outer segment region, whereas retinol removal is defined by the surface-to-volume ratio of the outer segment and the availability of retinoid binding protein (IRBP). The more rapid rates of retinol production in cones compared to rods are consistent with the more rapid operation of the visual cycle in these cells. |
format | Text |
id | pubmed-2151530 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21515302008-01-17 Visual Cycle: Dependence of Retinol Production and Removal on Photoproduct Decay and Cell Morphology Ala-Laurila, Petri Kolesnikov, Alexander V. Crouch, Rosalie K. Tsina, Efthymia Shukolyukov, Sergey A. Govardovskii, Victor I. Koutalos, Yiannis Wiggert, Barbara Estevez, Maureen E. Cornwall, M. Carter J Gen Physiol Articles The visual cycle is a chain of biochemical reactions that regenerate visual pigment following exposure to light. Initial steps, the liberation of all-trans retinal and its reduction to all-trans retinol by retinol dehydrogenase (RDH), take place in photoreceptors. We performed comparative microspectrophotometric and microfluorometric measurements on a variety of rod and cone photoreceptors isolated from salamander retinae to correlate the rates of photoproduct decay and retinol production. Metapigment decay rate was spatially uniform within outer segments and 50–70 times faster in the cells that contained cone-type pigment (SWS2 and M/LWS) compared to cells with rod-type pigment (RH1). Retinol production rate was strongly position dependent, fastest at the base of outer segments. Retinol production rate was 10–40 times faster in cones with cone pigments (SWS2 and M/LWS) than in the basal OS of rods containing rod pigment (RH1). Production rate was approximately five times faster in rods containing cone pigment (SWS2) than the rate in basal OS of rods containing the rod pigment (RH1). We show that retinol production is defined either by metapigment decay rate or RDH reaction rate, depending on cell type or outer segment region, whereas retinol removal is defined by the surface-to-volume ratio of the outer segment and the availability of retinoid binding protein (IRBP). The more rapid rates of retinol production in cones compared to rods are consistent with the more rapid operation of the visual cycle in these cells. The Rockefeller University Press 2006-08 /pmc/articles/PMC2151530/ /pubmed/16847097 http://dx.doi.org/10.1085/jgp.200609557 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Ala-Laurila, Petri Kolesnikov, Alexander V. Crouch, Rosalie K. Tsina, Efthymia Shukolyukov, Sergey A. Govardovskii, Victor I. Koutalos, Yiannis Wiggert, Barbara Estevez, Maureen E. Cornwall, M. Carter Visual Cycle: Dependence of Retinol Production and Removal on Photoproduct Decay and Cell Morphology |
title | Visual Cycle: Dependence of Retinol Production and Removal on Photoproduct Decay and Cell Morphology |
title_full | Visual Cycle: Dependence of Retinol Production and Removal on Photoproduct Decay and Cell Morphology |
title_fullStr | Visual Cycle: Dependence of Retinol Production and Removal on Photoproduct Decay and Cell Morphology |
title_full_unstemmed | Visual Cycle: Dependence of Retinol Production and Removal on Photoproduct Decay and Cell Morphology |
title_short | Visual Cycle: Dependence of Retinol Production and Removal on Photoproduct Decay and Cell Morphology |
title_sort | visual cycle: dependence of retinol production and removal on photoproduct decay and cell morphology |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151530/ https://www.ncbi.nlm.nih.gov/pubmed/16847097 http://dx.doi.org/10.1085/jgp.200609557 |
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