Cargando…

Visual Cycle: Dependence of Retinol Production and Removal on Photoproduct Decay and Cell Morphology

The visual cycle is a chain of biochemical reactions that regenerate visual pigment following exposure to light. Initial steps, the liberation of all-trans retinal and its reduction to all-trans retinol by retinol dehydrogenase (RDH), take place in photoreceptors. We performed comparative microspect...

Descripción completa

Detalles Bibliográficos
Autores principales: Ala-Laurila, Petri, Kolesnikov, Alexander V., Crouch, Rosalie K., Tsina, Efthymia, Shukolyukov, Sergey A., Govardovskii, Victor I., Koutalos, Yiannis, Wiggert, Barbara, Estevez, Maureen E., Cornwall, M. Carter
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151530/
https://www.ncbi.nlm.nih.gov/pubmed/16847097
http://dx.doi.org/10.1085/jgp.200609557
_version_ 1782144735570296832
author Ala-Laurila, Petri
Kolesnikov, Alexander V.
Crouch, Rosalie K.
Tsina, Efthymia
Shukolyukov, Sergey A.
Govardovskii, Victor I.
Koutalos, Yiannis
Wiggert, Barbara
Estevez, Maureen E.
Cornwall, M. Carter
author_facet Ala-Laurila, Petri
Kolesnikov, Alexander V.
Crouch, Rosalie K.
Tsina, Efthymia
Shukolyukov, Sergey A.
Govardovskii, Victor I.
Koutalos, Yiannis
Wiggert, Barbara
Estevez, Maureen E.
Cornwall, M. Carter
author_sort Ala-Laurila, Petri
collection PubMed
description The visual cycle is a chain of biochemical reactions that regenerate visual pigment following exposure to light. Initial steps, the liberation of all-trans retinal and its reduction to all-trans retinol by retinol dehydrogenase (RDH), take place in photoreceptors. We performed comparative microspectrophotometric and microfluorometric measurements on a variety of rod and cone photoreceptors isolated from salamander retinae to correlate the rates of photoproduct decay and retinol production. Metapigment decay rate was spatially uniform within outer segments and 50–70 times faster in the cells that contained cone-type pigment (SWS2 and M/LWS) compared to cells with rod-type pigment (RH1). Retinol production rate was strongly position dependent, fastest at the base of outer segments. Retinol production rate was 10–40 times faster in cones with cone pigments (SWS2 and M/LWS) than in the basal OS of rods containing rod pigment (RH1). Production rate was approximately five times faster in rods containing cone pigment (SWS2) than the rate in basal OS of rods containing the rod pigment (RH1). We show that retinol production is defined either by metapigment decay rate or RDH reaction rate, depending on cell type or outer segment region, whereas retinol removal is defined by the surface-to-volume ratio of the outer segment and the availability of retinoid binding protein (IRBP). The more rapid rates of retinol production in cones compared to rods are consistent with the more rapid operation of the visual cycle in these cells.
format Text
id pubmed-2151530
institution National Center for Biotechnology Information
language English
publishDate 2006
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21515302008-01-17 Visual Cycle: Dependence of Retinol Production and Removal on Photoproduct Decay and Cell Morphology Ala-Laurila, Petri Kolesnikov, Alexander V. Crouch, Rosalie K. Tsina, Efthymia Shukolyukov, Sergey A. Govardovskii, Victor I. Koutalos, Yiannis Wiggert, Barbara Estevez, Maureen E. Cornwall, M. Carter J Gen Physiol Articles The visual cycle is a chain of biochemical reactions that regenerate visual pigment following exposure to light. Initial steps, the liberation of all-trans retinal and its reduction to all-trans retinol by retinol dehydrogenase (RDH), take place in photoreceptors. We performed comparative microspectrophotometric and microfluorometric measurements on a variety of rod and cone photoreceptors isolated from salamander retinae to correlate the rates of photoproduct decay and retinol production. Metapigment decay rate was spatially uniform within outer segments and 50–70 times faster in the cells that contained cone-type pigment (SWS2 and M/LWS) compared to cells with rod-type pigment (RH1). Retinol production rate was strongly position dependent, fastest at the base of outer segments. Retinol production rate was 10–40 times faster in cones with cone pigments (SWS2 and M/LWS) than in the basal OS of rods containing rod pigment (RH1). Production rate was approximately five times faster in rods containing cone pigment (SWS2) than the rate in basal OS of rods containing the rod pigment (RH1). We show that retinol production is defined either by metapigment decay rate or RDH reaction rate, depending on cell type or outer segment region, whereas retinol removal is defined by the surface-to-volume ratio of the outer segment and the availability of retinoid binding protein (IRBP). The more rapid rates of retinol production in cones compared to rods are consistent with the more rapid operation of the visual cycle in these cells. The Rockefeller University Press 2006-08 /pmc/articles/PMC2151530/ /pubmed/16847097 http://dx.doi.org/10.1085/jgp.200609557 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Ala-Laurila, Petri
Kolesnikov, Alexander V.
Crouch, Rosalie K.
Tsina, Efthymia
Shukolyukov, Sergey A.
Govardovskii, Victor I.
Koutalos, Yiannis
Wiggert, Barbara
Estevez, Maureen E.
Cornwall, M. Carter
Visual Cycle: Dependence of Retinol Production and Removal on Photoproduct Decay and Cell Morphology
title Visual Cycle: Dependence of Retinol Production and Removal on Photoproduct Decay and Cell Morphology
title_full Visual Cycle: Dependence of Retinol Production and Removal on Photoproduct Decay and Cell Morphology
title_fullStr Visual Cycle: Dependence of Retinol Production and Removal on Photoproduct Decay and Cell Morphology
title_full_unstemmed Visual Cycle: Dependence of Retinol Production and Removal on Photoproduct Decay and Cell Morphology
title_short Visual Cycle: Dependence of Retinol Production and Removal on Photoproduct Decay and Cell Morphology
title_sort visual cycle: dependence of retinol production and removal on photoproduct decay and cell morphology
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151530/
https://www.ncbi.nlm.nih.gov/pubmed/16847097
http://dx.doi.org/10.1085/jgp.200609557
work_keys_str_mv AT alalaurilapetri visualcycledependenceofretinolproductionandremovalonphotoproductdecayandcellmorphology
AT kolesnikovalexanderv visualcycledependenceofretinolproductionandremovalonphotoproductdecayandcellmorphology
AT crouchrosaliek visualcycledependenceofretinolproductionandremovalonphotoproductdecayandcellmorphology
AT tsinaefthymia visualcycledependenceofretinolproductionandremovalonphotoproductdecayandcellmorphology
AT shukolyukovsergeya visualcycledependenceofretinolproductionandremovalonphotoproductdecayandcellmorphology
AT govardovskiivictori visualcycledependenceofretinolproductionandremovalonphotoproductdecayandcellmorphology
AT koutalosyiannis visualcycledependenceofretinolproductionandremovalonphotoproductdecayandcellmorphology
AT wiggertbarbara visualcycledependenceofretinolproductionandremovalonphotoproductdecayandcellmorphology
AT estevezmaureene visualcycledependenceofretinolproductionandremovalonphotoproductdecayandcellmorphology
AT cornwallmcarter visualcycledependenceofretinolproductionandremovalonphotoproductdecayandcellmorphology