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Pediatric Measles Vaccine Expressing a Dengue Antigen Induces Durable Serotype-specific Neutralizing Antibodies to Dengue Virus

Dengue disease is an increasing global health problem that threatens one-third of the world's population. Despite decades of efforts, no licensed vaccine against dengue is available. With the aim to develop an affordable vaccine that could be used in young populations living in tropical areas,...

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Autores principales: Brandler, Samantha, Lucas-Hourani, Marianne, Moris, Arnaud, Frenkiel, Marie-Pascale, Combredet, Chantal, Février, Michèle, Bedouelle, Hugues, Schwartz, Olivier, Desprès, Philippe, Tangy, Frédéric
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2154386/
https://www.ncbi.nlm.nih.gov/pubmed/18160988
http://dx.doi.org/10.1371/journal.pntd.0000096
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author Brandler, Samantha
Lucas-Hourani, Marianne
Moris, Arnaud
Frenkiel, Marie-Pascale
Combredet, Chantal
Février, Michèle
Bedouelle, Hugues
Schwartz, Olivier
Desprès, Philippe
Tangy, Frédéric
author_facet Brandler, Samantha
Lucas-Hourani, Marianne
Moris, Arnaud
Frenkiel, Marie-Pascale
Combredet, Chantal
Février, Michèle
Bedouelle, Hugues
Schwartz, Olivier
Desprès, Philippe
Tangy, Frédéric
author_sort Brandler, Samantha
collection PubMed
description Dengue disease is an increasing global health problem that threatens one-third of the world's population. Despite decades of efforts, no licensed vaccine against dengue is available. With the aim to develop an affordable vaccine that could be used in young populations living in tropical areas, we evaluated a new strategy based on the expression of a minimal dengue antigen by a vector derived from pediatric live-attenuated Schwarz measles vaccine (MV). As a proof-of-concept, we inserted into the MV vector a sequence encoding a minimal combined dengue antigen composed of the envelope domain III (EDIII) fused to the ectodomain of the membrane protein (ectoM) from DV serotype-1. Immunization of mice susceptible to MV resulted in a long-term production of DV1 serotype-specific neutralizing antibodies. The presence of ectoM was critical to the immunogenicity of inserted EDIII. The adjuvant capacity of ectoM correlated with its ability to promote the maturation of dendritic cells and the secretion of proinflammatory and antiviral cytokines and chemokines involved in adaptive immunity. The protective efficacy of this vaccine should be studied in non-human primates. A combined measles–dengue vaccine might provide a one-shot approach to immunize children against both diseases where they co-exist.
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spelling pubmed-21543862007-12-27 Pediatric Measles Vaccine Expressing a Dengue Antigen Induces Durable Serotype-specific Neutralizing Antibodies to Dengue Virus Brandler, Samantha Lucas-Hourani, Marianne Moris, Arnaud Frenkiel, Marie-Pascale Combredet, Chantal Février, Michèle Bedouelle, Hugues Schwartz, Olivier Desprès, Philippe Tangy, Frédéric PLoS Negl Trop Dis Research Article Dengue disease is an increasing global health problem that threatens one-third of the world's population. Despite decades of efforts, no licensed vaccine against dengue is available. With the aim to develop an affordable vaccine that could be used in young populations living in tropical areas, we evaluated a new strategy based on the expression of a minimal dengue antigen by a vector derived from pediatric live-attenuated Schwarz measles vaccine (MV). As a proof-of-concept, we inserted into the MV vector a sequence encoding a minimal combined dengue antigen composed of the envelope domain III (EDIII) fused to the ectodomain of the membrane protein (ectoM) from DV serotype-1. Immunization of mice susceptible to MV resulted in a long-term production of DV1 serotype-specific neutralizing antibodies. The presence of ectoM was critical to the immunogenicity of inserted EDIII. The adjuvant capacity of ectoM correlated with its ability to promote the maturation of dendritic cells and the secretion of proinflammatory and antiviral cytokines and chemokines involved in adaptive immunity. The protective efficacy of this vaccine should be studied in non-human primates. A combined measles–dengue vaccine might provide a one-shot approach to immunize children against both diseases where they co-exist. Public Library of Science 2007-12-12 /pmc/articles/PMC2154386/ /pubmed/18160988 http://dx.doi.org/10.1371/journal.pntd.0000096 Text en Brandler et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Brandler, Samantha
Lucas-Hourani, Marianne
Moris, Arnaud
Frenkiel, Marie-Pascale
Combredet, Chantal
Février, Michèle
Bedouelle, Hugues
Schwartz, Olivier
Desprès, Philippe
Tangy, Frédéric
Pediatric Measles Vaccine Expressing a Dengue Antigen Induces Durable Serotype-specific Neutralizing Antibodies to Dengue Virus
title Pediatric Measles Vaccine Expressing a Dengue Antigen Induces Durable Serotype-specific Neutralizing Antibodies to Dengue Virus
title_full Pediatric Measles Vaccine Expressing a Dengue Antigen Induces Durable Serotype-specific Neutralizing Antibodies to Dengue Virus
title_fullStr Pediatric Measles Vaccine Expressing a Dengue Antigen Induces Durable Serotype-specific Neutralizing Antibodies to Dengue Virus
title_full_unstemmed Pediatric Measles Vaccine Expressing a Dengue Antigen Induces Durable Serotype-specific Neutralizing Antibodies to Dengue Virus
title_short Pediatric Measles Vaccine Expressing a Dengue Antigen Induces Durable Serotype-specific Neutralizing Antibodies to Dengue Virus
title_sort pediatric measles vaccine expressing a dengue antigen induces durable serotype-specific neutralizing antibodies to dengue virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2154386/
https://www.ncbi.nlm.nih.gov/pubmed/18160988
http://dx.doi.org/10.1371/journal.pntd.0000096
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