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Regulation of APC Activity by Phosphorylation and Regulatory Factors
Ubiquitin-dependent proteolysis of Cut2/Pds1 and Cyclin B is required for sister chromatid separation and exit from mitosis, respectively. Anaphase-promoting complex/cyclosome (APC) specifically ubiquitinates Cut2/Pds1 at metaphase–anaphase transition, and ubiquitinates Cyclin B in late mitosis and...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1999
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2156135/ https://www.ncbi.nlm.nih.gov/pubmed/10459014 |
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author | Kotani, Shuji Tanaka, Hirofumi Yasuda, Hideyo Todokoro, Kazuo |
author_facet | Kotani, Shuji Tanaka, Hirofumi Yasuda, Hideyo Todokoro, Kazuo |
author_sort | Kotani, Shuji |
collection | PubMed |
description | Ubiquitin-dependent proteolysis of Cut2/Pds1 and Cyclin B is required for sister chromatid separation and exit from mitosis, respectively. Anaphase-promoting complex/cyclosome (APC) specifically ubiquitinates Cut2/Pds1 at metaphase–anaphase transition, and ubiquitinates Cyclin B in late mitosis and G1 phase. However, the exact regulatory mechanism of substrate-specific activation of mammalian APC with the right timing remains to be elucidated. We found that not only the binding of the activators Cdc20 and Cdh1 and the inhibitor Mad2 to APC, but also the phosphorylation of Cdc20 and Cdh1 by Cdc2-Cyclin B and that of APC by Polo-like kinase and cAMP-dependent protein kinase, regulate APC activity. The cooperation of the phosphorylation/dephosphorylation and the regulatory factors in regulation of APC activity may thus control the precise progression of mitosis. |
format | Text |
id | pubmed-2156135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1999 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21561352008-05-01 Regulation of APC Activity by Phosphorylation and Regulatory Factors Kotani, Shuji Tanaka, Hirofumi Yasuda, Hideyo Todokoro, Kazuo J Cell Biol Original Article Ubiquitin-dependent proteolysis of Cut2/Pds1 and Cyclin B is required for sister chromatid separation and exit from mitosis, respectively. Anaphase-promoting complex/cyclosome (APC) specifically ubiquitinates Cut2/Pds1 at metaphase–anaphase transition, and ubiquitinates Cyclin B in late mitosis and G1 phase. However, the exact regulatory mechanism of substrate-specific activation of mammalian APC with the right timing remains to be elucidated. We found that not only the binding of the activators Cdc20 and Cdh1 and the inhibitor Mad2 to APC, but also the phosphorylation of Cdc20 and Cdh1 by Cdc2-Cyclin B and that of APC by Polo-like kinase and cAMP-dependent protein kinase, regulate APC activity. The cooperation of the phosphorylation/dephosphorylation and the regulatory factors in regulation of APC activity may thus control the precise progression of mitosis. The Rockefeller University Press 1999-08-23 /pmc/articles/PMC2156135/ /pubmed/10459014 Text en © 1999 The Rockefeller University Press https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/ (https://creativecommons.org/licenses/by-nc-sa/4.0/) ). |
spellingShingle | Original Article Kotani, Shuji Tanaka, Hirofumi Yasuda, Hideyo Todokoro, Kazuo Regulation of APC Activity by Phosphorylation and Regulatory Factors |
title | Regulation of APC Activity by Phosphorylation and Regulatory Factors |
title_full | Regulation of APC Activity by Phosphorylation and Regulatory Factors |
title_fullStr | Regulation of APC Activity by Phosphorylation and Regulatory Factors |
title_full_unstemmed | Regulation of APC Activity by Phosphorylation and Regulatory Factors |
title_short | Regulation of APC Activity by Phosphorylation and Regulatory Factors |
title_sort | regulation of apc activity by phosphorylation and regulatory factors |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2156135/ https://www.ncbi.nlm.nih.gov/pubmed/10459014 |
work_keys_str_mv | AT kotanishuji regulationofapcactivitybyphosphorylationandregulatoryfactors AT tanakahirofumi regulationofapcactivitybyphosphorylationandregulatoryfactors AT yasudahideyo regulationofapcactivitybyphosphorylationandregulatoryfactors AT todokorokazuo regulationofapcactivitybyphosphorylationandregulatoryfactors |