Cargando…

Sid2p, a Spindle Pole Body Kinase That Regulates the Onset of Cytokinesis

The fission yeast Schizosaccharomyces pombe divides by medial fission through the use of an actomyosin contractile ring. Precisely at the end of anaphase, the ring begins to constrict and the septum forms. Proper coordination of cell division with mitosis is crucial to ensure proper segregation of c...

Descripción completa

Detalles Bibliográficos
Autores principales: Sparks, Cynthia A., Morphew, Mary, McCollum, Dannel
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2156147/
https://www.ncbi.nlm.nih.gov/pubmed/10459013
_version_ 1782144815564062720
author Sparks, Cynthia A.
Morphew, Mary
McCollum, Dannel
author_facet Sparks, Cynthia A.
Morphew, Mary
McCollum, Dannel
author_sort Sparks, Cynthia A.
collection PubMed
description The fission yeast Schizosaccharomyces pombe divides by medial fission through the use of an actomyosin contractile ring. Precisely at the end of anaphase, the ring begins to constrict and the septum forms. Proper coordination of cell division with mitosis is crucial to ensure proper segregation of chromosomes to daughter cells. The Sid2p kinase is one of several proteins that function as part of a novel signaling pathway required for initiation of medial ring constriction and septation. Here, we show that Sid2p is a component of the spindle pole body at all stages of the cell cycle and localizes transiently to the cell division site during medial ring constriction and septation. A medial ring and an intact microtubule cytoskeleton are required for the localization of Sid2p to the division site. We have established an in vitro assay for measuring Sid2p kinase activity, and found that Sid2p kinase activity peaks during medial ring constriction and septation. Both Sid2p localization to the division site and activity depend on the function of all of the other septation initiation genes: cdc7, cdc11, cdc14, sid1, spg1, and sid4. Thus, Sid2p, a component of the spindle pole body, by virtue of its transient localization to the division site, appears to determine the timing of ring constriction and septum delivery in response to activating signals from other Sid gene products.
format Text
id pubmed-2156147
institution National Center for Biotechnology Information
language English
publishDate 1999
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21561472008-05-01 Sid2p, a Spindle Pole Body Kinase That Regulates the Onset of Cytokinesis Sparks, Cynthia A. Morphew, Mary McCollum, Dannel J Cell Biol Original Article The fission yeast Schizosaccharomyces pombe divides by medial fission through the use of an actomyosin contractile ring. Precisely at the end of anaphase, the ring begins to constrict and the septum forms. Proper coordination of cell division with mitosis is crucial to ensure proper segregation of chromosomes to daughter cells. The Sid2p kinase is one of several proteins that function as part of a novel signaling pathway required for initiation of medial ring constriction and septation. Here, we show that Sid2p is a component of the spindle pole body at all stages of the cell cycle and localizes transiently to the cell division site during medial ring constriction and septation. A medial ring and an intact microtubule cytoskeleton are required for the localization of Sid2p to the division site. We have established an in vitro assay for measuring Sid2p kinase activity, and found that Sid2p kinase activity peaks during medial ring constriction and septation. Both Sid2p localization to the division site and activity depend on the function of all of the other septation initiation genes: cdc7, cdc11, cdc14, sid1, spg1, and sid4. Thus, Sid2p, a component of the spindle pole body, by virtue of its transient localization to the division site, appears to determine the timing of ring constriction and septum delivery in response to activating signals from other Sid gene products. The Rockefeller University Press 1999-08-23 /pmc/articles/PMC2156147/ /pubmed/10459013 Text en © 1999 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Sparks, Cynthia A.
Morphew, Mary
McCollum, Dannel
Sid2p, a Spindle Pole Body Kinase That Regulates the Onset of Cytokinesis
title Sid2p, a Spindle Pole Body Kinase That Regulates the Onset of Cytokinesis
title_full Sid2p, a Spindle Pole Body Kinase That Regulates the Onset of Cytokinesis
title_fullStr Sid2p, a Spindle Pole Body Kinase That Regulates the Onset of Cytokinesis
title_full_unstemmed Sid2p, a Spindle Pole Body Kinase That Regulates the Onset of Cytokinesis
title_short Sid2p, a Spindle Pole Body Kinase That Regulates the Onset of Cytokinesis
title_sort sid2p, a spindle pole body kinase that regulates the onset of cytokinesis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2156147/
https://www.ncbi.nlm.nih.gov/pubmed/10459013
work_keys_str_mv AT sparkscynthiaa sid2paspindlepolebodykinasethatregulatestheonsetofcytokinesis
AT morphewmary sid2paspindlepolebodykinasethatregulatestheonsetofcytokinesis
AT mccollumdannel sid2paspindlepolebodykinasethatregulatestheonsetofcytokinesis