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Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells
We visualized a fluorescent-protein (FP) fusion to Rab6, a Golgi-associated GTPase, in conjunction with fluorescent secretory pathway markers. FP-Rab6 defined highly dynamic transport carriers (TCs) translocating from the Golgi to the cell periphery. FP-Rab6 TCs specifically accumulated a retrograde...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1999
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2156170/ https://www.ncbi.nlm.nih.gov/pubmed/10562278 |
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author | White, Jamie Johannes, Ludger Mallard, Frédéric Girod, Andreas Grill, Stephan Reinsch, Sigrid Keller, Patrick Tzschaschel, Barbara Echard, Arnaud Goud, Bruno Stelzer, Ernst H.K. |
author_facet | White, Jamie Johannes, Ludger Mallard, Frédéric Girod, Andreas Grill, Stephan Reinsch, Sigrid Keller, Patrick Tzschaschel, Barbara Echard, Arnaud Goud, Bruno Stelzer, Ernst H.K. |
author_sort | White, Jamie |
collection | PubMed |
description | We visualized a fluorescent-protein (FP) fusion to Rab6, a Golgi-associated GTPase, in conjunction with fluorescent secretory pathway markers. FP-Rab6 defined highly dynamic transport carriers (TCs) translocating from the Golgi to the cell periphery. FP-Rab6 TCs specifically accumulated a retrograde cargo, the wild-type Shiga toxin B-fragment (STB), during STB transport from the Golgi to the endoplasmic reticulum (ER). FP-Rab6 TCs associated intimately with the ER, and STB entered the ER via specialized peripheral regions that accumulated FP-Rab6. Microinjection of antibodies that block coatomer protein I (COPI) function inhibited trafficking of a KDEL-receptor FP-fusion, but not FP-Rab6. Additionally, markers of COPI-dependent recycling were excluded from FP-Rab6/STB TCs. Overexpression of Rab6:GDP (T27N mutant) using T7 vaccinia inhibited toxicity of Shiga holotoxin, but did not alter STB transport to the Golgi or Golgi morphology. Taken together, our results indicate Rab6 regulates a novel Golgi to ER transport pathway. |
format | Text |
id | pubmed-2156170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1999 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21561702008-05-01 Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells White, Jamie Johannes, Ludger Mallard, Frédéric Girod, Andreas Grill, Stephan Reinsch, Sigrid Keller, Patrick Tzschaschel, Barbara Echard, Arnaud Goud, Bruno Stelzer, Ernst H.K. J Cell Biol Original Article We visualized a fluorescent-protein (FP) fusion to Rab6, a Golgi-associated GTPase, in conjunction with fluorescent secretory pathway markers. FP-Rab6 defined highly dynamic transport carriers (TCs) translocating from the Golgi to the cell periphery. FP-Rab6 TCs specifically accumulated a retrograde cargo, the wild-type Shiga toxin B-fragment (STB), during STB transport from the Golgi to the endoplasmic reticulum (ER). FP-Rab6 TCs associated intimately with the ER, and STB entered the ER via specialized peripheral regions that accumulated FP-Rab6. Microinjection of antibodies that block coatomer protein I (COPI) function inhibited trafficking of a KDEL-receptor FP-fusion, but not FP-Rab6. Additionally, markers of COPI-dependent recycling were excluded from FP-Rab6/STB TCs. Overexpression of Rab6:GDP (T27N mutant) using T7 vaccinia inhibited toxicity of Shiga holotoxin, but did not alter STB transport to the Golgi or Golgi morphology. Taken together, our results indicate Rab6 regulates a novel Golgi to ER transport pathway. The Rockefeller University Press 1999-11-15 /pmc/articles/PMC2156170/ /pubmed/10562278 Text en © 1999 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Original Article White, Jamie Johannes, Ludger Mallard, Frédéric Girod, Andreas Grill, Stephan Reinsch, Sigrid Keller, Patrick Tzschaschel, Barbara Echard, Arnaud Goud, Bruno Stelzer, Ernst H.K. Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells |
title | Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells |
title_full | Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells |
title_fullStr | Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells |
title_full_unstemmed | Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells |
title_short | Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells |
title_sort | rab6 coordinates a novel golgi to er retrograde transport pathway in live cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2156170/ https://www.ncbi.nlm.nih.gov/pubmed/10562278 |
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