Cargando…

Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells

We visualized a fluorescent-protein (FP) fusion to Rab6, a Golgi-associated GTPase, in conjunction with fluorescent secretory pathway markers. FP-Rab6 defined highly dynamic transport carriers (TCs) translocating from the Golgi to the cell periphery. FP-Rab6 TCs specifically accumulated a retrograde...

Descripción completa

Detalles Bibliográficos
Autores principales: White, Jamie, Johannes, Ludger, Mallard, Frédéric, Girod, Andreas, Grill, Stephan, Reinsch, Sigrid, Keller, Patrick, Tzschaschel, Barbara, Echard, Arnaud, Goud, Bruno, Stelzer, Ernst H.K.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2156170/
https://www.ncbi.nlm.nih.gov/pubmed/10562278
_version_ 1782144820754513920
author White, Jamie
Johannes, Ludger
Mallard, Frédéric
Girod, Andreas
Grill, Stephan
Reinsch, Sigrid
Keller, Patrick
Tzschaschel, Barbara
Echard, Arnaud
Goud, Bruno
Stelzer, Ernst H.K.
author_facet White, Jamie
Johannes, Ludger
Mallard, Frédéric
Girod, Andreas
Grill, Stephan
Reinsch, Sigrid
Keller, Patrick
Tzschaschel, Barbara
Echard, Arnaud
Goud, Bruno
Stelzer, Ernst H.K.
author_sort White, Jamie
collection PubMed
description We visualized a fluorescent-protein (FP) fusion to Rab6, a Golgi-associated GTPase, in conjunction with fluorescent secretory pathway markers. FP-Rab6 defined highly dynamic transport carriers (TCs) translocating from the Golgi to the cell periphery. FP-Rab6 TCs specifically accumulated a retrograde cargo, the wild-type Shiga toxin B-fragment (STB), during STB transport from the Golgi to the endoplasmic reticulum (ER). FP-Rab6 TCs associated intimately with the ER, and STB entered the ER via specialized peripheral regions that accumulated FP-Rab6. Microinjection of antibodies that block coatomer protein I (COPI) function inhibited trafficking of a KDEL-receptor FP-fusion, but not FP-Rab6. Additionally, markers of COPI-dependent recycling were excluded from FP-Rab6/STB TCs. Overexpression of Rab6:GDP (T27N mutant) using T7 vaccinia inhibited toxicity of Shiga holotoxin, but did not alter STB transport to the Golgi or Golgi morphology. Taken together, our results indicate Rab6 regulates a novel Golgi to ER transport pathway.
format Text
id pubmed-2156170
institution National Center for Biotechnology Information
language English
publishDate 1999
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21561702008-05-01 Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells White, Jamie Johannes, Ludger Mallard, Frédéric Girod, Andreas Grill, Stephan Reinsch, Sigrid Keller, Patrick Tzschaschel, Barbara Echard, Arnaud Goud, Bruno Stelzer, Ernst H.K. J Cell Biol Original Article We visualized a fluorescent-protein (FP) fusion to Rab6, a Golgi-associated GTPase, in conjunction with fluorescent secretory pathway markers. FP-Rab6 defined highly dynamic transport carriers (TCs) translocating from the Golgi to the cell periphery. FP-Rab6 TCs specifically accumulated a retrograde cargo, the wild-type Shiga toxin B-fragment (STB), during STB transport from the Golgi to the endoplasmic reticulum (ER). FP-Rab6 TCs associated intimately with the ER, and STB entered the ER via specialized peripheral regions that accumulated FP-Rab6. Microinjection of antibodies that block coatomer protein I (COPI) function inhibited trafficking of a KDEL-receptor FP-fusion, but not FP-Rab6. Additionally, markers of COPI-dependent recycling were excluded from FP-Rab6/STB TCs. Overexpression of Rab6:GDP (T27N mutant) using T7 vaccinia inhibited toxicity of Shiga holotoxin, but did not alter STB transport to the Golgi or Golgi morphology. Taken together, our results indicate Rab6 regulates a novel Golgi to ER transport pathway. The Rockefeller University Press 1999-11-15 /pmc/articles/PMC2156170/ /pubmed/10562278 Text en © 1999 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
White, Jamie
Johannes, Ludger
Mallard, Frédéric
Girod, Andreas
Grill, Stephan
Reinsch, Sigrid
Keller, Patrick
Tzschaschel, Barbara
Echard, Arnaud
Goud, Bruno
Stelzer, Ernst H.K.
Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells
title Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells
title_full Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells
title_fullStr Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells
title_full_unstemmed Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells
title_short Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells
title_sort rab6 coordinates a novel golgi to er retrograde transport pathway in live cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2156170/
https://www.ncbi.nlm.nih.gov/pubmed/10562278
work_keys_str_mv AT whitejamie rab6coordinatesanovelgolgitoerretrogradetransportpathwayinlivecells
AT johannesludger rab6coordinatesanovelgolgitoerretrogradetransportpathwayinlivecells
AT mallardfrederic rab6coordinatesanovelgolgitoerretrogradetransportpathwayinlivecells
AT girodandreas rab6coordinatesanovelgolgitoerretrogradetransportpathwayinlivecells
AT grillstephan rab6coordinatesanovelgolgitoerretrogradetransportpathwayinlivecells
AT reinschsigrid rab6coordinatesanovelgolgitoerretrogradetransportpathwayinlivecells
AT kellerpatrick rab6coordinatesanovelgolgitoerretrogradetransportpathwayinlivecells
AT tzschaschelbarbara rab6coordinatesanovelgolgitoerretrogradetransportpathwayinlivecells
AT echardarnaud rab6coordinatesanovelgolgitoerretrogradetransportpathwayinlivecells
AT goudbruno rab6coordinatesanovelgolgitoerretrogradetransportpathwayinlivecells
AT stelzerernsthk rab6coordinatesanovelgolgitoerretrogradetransportpathwayinlivecells