Cargando…
Self-Assembling Peptide Nanofiber Scaffolds Accelerate Wound Healing
Cutaneous wound repair regenerates skin integrity, but a chronic failure to heal results in compromised tissue function and increased morbidity. To address this, we have used an integrated approach, using nanobiotechnology to augment the rate of wound reepithelialization by combining self-assembling...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2008
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2157486/ https://www.ncbi.nlm.nih.gov/pubmed/18183291 http://dx.doi.org/10.1371/journal.pone.0001410 |
_version_ | 1782144827794653184 |
---|---|
author | Schneider, Aurore Garlick, Jonathan A. Egles, Christophe |
author_facet | Schneider, Aurore Garlick, Jonathan A. Egles, Christophe |
author_sort | Schneider, Aurore |
collection | PubMed |
description | Cutaneous wound repair regenerates skin integrity, but a chronic failure to heal results in compromised tissue function and increased morbidity. To address this, we have used an integrated approach, using nanobiotechnology to augment the rate of wound reepithelialization by combining self-assembling peptide (SAP) nanofiber scaffold and Epidermal Growth Factor (EGF). This SAP bioscaffold was tested in a bioengineered Human Skin Equivalent (HSE) tissue model that enabled wound reepithelialization to be monitored in a tissue that recapitulates molecular and cellular mechanisms of repair known to occur in human skin. We found that SAP underwent molecular self-assembly to form unique 3D structures that stably covered the surface of the wound, suggesting that this scaffold may serve as a viable wound dressing. We measured the rates of release of EGF from the SAP scaffold and determined that EGF was only released when the scaffold was in direct contact with the HSE. By measuring the length of the epithelial tongue during wound reepithelialization, we found that SAP scaffolds containing EGF accelerated the rate of wound coverage by 5 fold when compared to controls without scaffolds and by 3.5 fold when compared to the scaffold without EGF. In conclusion, our experiments demonstrated that biomaterials composed of a biofunctionalized peptidic scaffold have many properties that are well-suited for the treatment of cutaneous wounds including wound coverage, functionalization with bioactive molecules, localized growth factor release and activation of wound repair. |
format | Text |
id | pubmed-2157486 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-21574862008-01-09 Self-Assembling Peptide Nanofiber Scaffolds Accelerate Wound Healing Schneider, Aurore Garlick, Jonathan A. Egles, Christophe PLoS One Research Article Cutaneous wound repair regenerates skin integrity, but a chronic failure to heal results in compromised tissue function and increased morbidity. To address this, we have used an integrated approach, using nanobiotechnology to augment the rate of wound reepithelialization by combining self-assembling peptide (SAP) nanofiber scaffold and Epidermal Growth Factor (EGF). This SAP bioscaffold was tested in a bioengineered Human Skin Equivalent (HSE) tissue model that enabled wound reepithelialization to be monitored in a tissue that recapitulates molecular and cellular mechanisms of repair known to occur in human skin. We found that SAP underwent molecular self-assembly to form unique 3D structures that stably covered the surface of the wound, suggesting that this scaffold may serve as a viable wound dressing. We measured the rates of release of EGF from the SAP scaffold and determined that EGF was only released when the scaffold was in direct contact with the HSE. By measuring the length of the epithelial tongue during wound reepithelialization, we found that SAP scaffolds containing EGF accelerated the rate of wound coverage by 5 fold when compared to controls without scaffolds and by 3.5 fold when compared to the scaffold without EGF. In conclusion, our experiments demonstrated that biomaterials composed of a biofunctionalized peptidic scaffold have many properties that are well-suited for the treatment of cutaneous wounds including wound coverage, functionalization with bioactive molecules, localized growth factor release and activation of wound repair. Public Library of Science 2008-01-09 /pmc/articles/PMC2157486/ /pubmed/18183291 http://dx.doi.org/10.1371/journal.pone.0001410 Text en Schneider et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Schneider, Aurore Garlick, Jonathan A. Egles, Christophe Self-Assembling Peptide Nanofiber Scaffolds Accelerate Wound Healing |
title | Self-Assembling Peptide Nanofiber Scaffolds Accelerate Wound Healing |
title_full | Self-Assembling Peptide Nanofiber Scaffolds Accelerate Wound Healing |
title_fullStr | Self-Assembling Peptide Nanofiber Scaffolds Accelerate Wound Healing |
title_full_unstemmed | Self-Assembling Peptide Nanofiber Scaffolds Accelerate Wound Healing |
title_short | Self-Assembling Peptide Nanofiber Scaffolds Accelerate Wound Healing |
title_sort | self-assembling peptide nanofiber scaffolds accelerate wound healing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2157486/ https://www.ncbi.nlm.nih.gov/pubmed/18183291 http://dx.doi.org/10.1371/journal.pone.0001410 |
work_keys_str_mv | AT schneideraurore selfassemblingpeptidenanofiberscaffoldsacceleratewoundhealing AT garlickjonathana selfassemblingpeptidenanofiberscaffoldsacceleratewoundhealing AT egleschristophe selfassemblingpeptidenanofiberscaffoldsacceleratewoundhealing |