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Afadin: A Key Molecule Essential for Structural Organization of Cell–Cell Junctions of Polarized Epithelia during Embryogenesis
Afadin is an actin filament–binding protein that binds to nectin, an immunoglobulin-like cell adhesion molecule, and is colocalized with nectin at cadherin-based cell–cell adherens junctions (AJs). To explore the function of afadin in cell–cell adhesion during embryogenesis, we generated afadin(−/−)...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1999
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2169488/ https://www.ncbi.nlm.nih.gov/pubmed/10477764 |
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author | Ikeda, Wataru Nakanishi, Hiroyuki Miyoshi, Jun Mandai, Kenji Ishizaki, Hiroyoshi Tanaka, Miki Togawa, Atushi Takahashi, Kenichi Nishioka, Hideo Yoshida, Hisahiro Mizoguchi, Akira Nishikawa, Shin-ichi Takai, Yoshimi |
author_facet | Ikeda, Wataru Nakanishi, Hiroyuki Miyoshi, Jun Mandai, Kenji Ishizaki, Hiroyoshi Tanaka, Miki Togawa, Atushi Takahashi, Kenichi Nishioka, Hideo Yoshida, Hisahiro Mizoguchi, Akira Nishikawa, Shin-ichi Takai, Yoshimi |
author_sort | Ikeda, Wataru |
collection | PubMed |
description | Afadin is an actin filament–binding protein that binds to nectin, an immunoglobulin-like cell adhesion molecule, and is colocalized with nectin at cadherin-based cell–cell adherens junctions (AJs). To explore the function of afadin in cell–cell adhesion during embryogenesis, we generated afadin(−/−) mice and embryonic stem cells. In wild-type mice at embryonic days 6.5–8.5, afadin was highly expressed in the embryonic ectoderm and the mesoderm, but hardly detected in the extraembryonic regions such as the visceral endoderm. Afadin(−/−) mice showed developmental defects at stages during and after gastrulation, including disorganization of the ectoderm, impaired migration of the mesoderm, and loss of somites and other structures derived from both the ectoderm and the mesoderm. Cystic embryoid bodies derived from afadin(−/−) embryonic stem cells showed normal organization of the endoderm but disorganization of the ectoderm. Cell–cell AJs and tight junctions were improperly organized in the ectoderm of afadin(−/−) mice and embryoid bodies. These results indicate that afadin is highly expressed in the ectoderm- derived cells during embryogenesis and plays a key role in proper organization of AJs and tight junctions of the highly expressing cells, which is essential for proper tissue morphogenesis. |
format | Text |
id | pubmed-2169488 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1999 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21694882008-05-01 Afadin: A Key Molecule Essential for Structural Organization of Cell–Cell Junctions of Polarized Epithelia during Embryogenesis Ikeda, Wataru Nakanishi, Hiroyuki Miyoshi, Jun Mandai, Kenji Ishizaki, Hiroyoshi Tanaka, Miki Togawa, Atushi Takahashi, Kenichi Nishioka, Hideo Yoshida, Hisahiro Mizoguchi, Akira Nishikawa, Shin-ichi Takai, Yoshimi J Cell Biol Original Article Afadin is an actin filament–binding protein that binds to nectin, an immunoglobulin-like cell adhesion molecule, and is colocalized with nectin at cadherin-based cell–cell adherens junctions (AJs). To explore the function of afadin in cell–cell adhesion during embryogenesis, we generated afadin(−/−) mice and embryonic stem cells. In wild-type mice at embryonic days 6.5–8.5, afadin was highly expressed in the embryonic ectoderm and the mesoderm, but hardly detected in the extraembryonic regions such as the visceral endoderm. Afadin(−/−) mice showed developmental defects at stages during and after gastrulation, including disorganization of the ectoderm, impaired migration of the mesoderm, and loss of somites and other structures derived from both the ectoderm and the mesoderm. Cystic embryoid bodies derived from afadin(−/−) embryonic stem cells showed normal organization of the endoderm but disorganization of the ectoderm. Cell–cell AJs and tight junctions were improperly organized in the ectoderm of afadin(−/−) mice and embryoid bodies. These results indicate that afadin is highly expressed in the ectoderm- derived cells during embryogenesis and plays a key role in proper organization of AJs and tight junctions of the highly expressing cells, which is essential for proper tissue morphogenesis. The Rockefeller University Press 1999-09-06 /pmc/articles/PMC2169488/ /pubmed/10477764 Text en © 1999 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Original Article Ikeda, Wataru Nakanishi, Hiroyuki Miyoshi, Jun Mandai, Kenji Ishizaki, Hiroyoshi Tanaka, Miki Togawa, Atushi Takahashi, Kenichi Nishioka, Hideo Yoshida, Hisahiro Mizoguchi, Akira Nishikawa, Shin-ichi Takai, Yoshimi Afadin: A Key Molecule Essential for Structural Organization of Cell–Cell Junctions of Polarized Epithelia during Embryogenesis |
title | Afadin: A Key Molecule Essential for Structural Organization of Cell–Cell Junctions of Polarized Epithelia during Embryogenesis |
title_full | Afadin: A Key Molecule Essential for Structural Organization of Cell–Cell Junctions of Polarized Epithelia during Embryogenesis |
title_fullStr | Afadin: A Key Molecule Essential for Structural Organization of Cell–Cell Junctions of Polarized Epithelia during Embryogenesis |
title_full_unstemmed | Afadin: A Key Molecule Essential for Structural Organization of Cell–Cell Junctions of Polarized Epithelia during Embryogenesis |
title_short | Afadin: A Key Molecule Essential for Structural Organization of Cell–Cell Junctions of Polarized Epithelia during Embryogenesis |
title_sort | afadin: a key molecule essential for structural organization of cell–cell junctions of polarized epithelia during embryogenesis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2169488/ https://www.ncbi.nlm.nih.gov/pubmed/10477764 |
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