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Melanophilin and myosin Va track the microtubule plus end on EB1
In mouse melanocytes, myosin Va is recruited onto the surface of melanosomes by a receptor complex containing Rab27a that is present in the melanosome membrane and melanophilin (Mlp), which links myosin Va to Rab27a. In this study, we show that Mlp is also a microtubule plus end–tracking protein or...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171176/ https://www.ncbi.nlm.nih.gov/pubmed/16247022 http://dx.doi.org/10.1083/jcb.200503028 |
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author | Wu, Xufeng S. Tsan, Grace L. Hammer, John A. |
author_facet | Wu, Xufeng S. Tsan, Grace L. Hammer, John A. |
author_sort | Wu, Xufeng S. |
collection | PubMed |
description | In mouse melanocytes, myosin Va is recruited onto the surface of melanosomes by a receptor complex containing Rab27a that is present in the melanosome membrane and melanophilin (Mlp), which links myosin Va to Rab27a. In this study, we show that Mlp is also a microtubule plus end–tracking protein or +TIP. Moreover, myosin Va tracks the plus end in a Mlp-dependent manner. Data showing that overexpression and short inhibitory RNA knockdown of the +TIP EB1 have opposite effects on Mlp–microtubule interaction, that Mlp interacts directly with EB1, and that deletion from Mlp of a region similar to one in the adenomatous polyposis coli protein involved in EB1 binding blocks Mlp's ability to plus end track argue that Mlp tracks the plus end directly by hitchhiking on EB1. These results identify a novel +TIP and indicate that vertebrate cells possess a +TIP complex that is similar to the Myo2p–Kar9p–Bim1p complex in yeast. We suggest that the +TIP complex identified in this study may serve to focus the transfer of melanosomes from microtubules to actin at the microtubule plus end. |
format | Text |
id | pubmed-2171176 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21711762008-03-05 Melanophilin and myosin Va track the microtubule plus end on EB1 Wu, Xufeng S. Tsan, Grace L. Hammer, John A. J Cell Biol Research Articles In mouse melanocytes, myosin Va is recruited onto the surface of melanosomes by a receptor complex containing Rab27a that is present in the melanosome membrane and melanophilin (Mlp), which links myosin Va to Rab27a. In this study, we show that Mlp is also a microtubule plus end–tracking protein or +TIP. Moreover, myosin Va tracks the plus end in a Mlp-dependent manner. Data showing that overexpression and short inhibitory RNA knockdown of the +TIP EB1 have opposite effects on Mlp–microtubule interaction, that Mlp interacts directly with EB1, and that deletion from Mlp of a region similar to one in the adenomatous polyposis coli protein involved in EB1 binding blocks Mlp's ability to plus end track argue that Mlp tracks the plus end directly by hitchhiking on EB1. These results identify a novel +TIP and indicate that vertebrate cells possess a +TIP complex that is similar to the Myo2p–Kar9p–Bim1p complex in yeast. We suggest that the +TIP complex identified in this study may serve to focus the transfer of melanosomes from microtubules to actin at the microtubule plus end. The Rockefeller University Press 2005-10-24 /pmc/articles/PMC2171176/ /pubmed/16247022 http://dx.doi.org/10.1083/jcb.200503028 Text en Copyright © 2005, Government This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Wu, Xufeng S. Tsan, Grace L. Hammer, John A. Melanophilin and myosin Va track the microtubule plus end on EB1 |
title | Melanophilin and myosin Va track the microtubule plus end on EB1 |
title_full | Melanophilin and myosin Va track the microtubule plus end on EB1 |
title_fullStr | Melanophilin and myosin Va track the microtubule plus end on EB1 |
title_full_unstemmed | Melanophilin and myosin Va track the microtubule plus end on EB1 |
title_short | Melanophilin and myosin Va track the microtubule plus end on EB1 |
title_sort | melanophilin and myosin va track the microtubule plus end on eb1 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171176/ https://www.ncbi.nlm.nih.gov/pubmed/16247022 http://dx.doi.org/10.1083/jcb.200503028 |
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