Cargando…

Melanophilin and myosin Va track the microtubule plus end on EB1

In mouse melanocytes, myosin Va is recruited onto the surface of melanosomes by a receptor complex containing Rab27a that is present in the melanosome membrane and melanophilin (Mlp), which links myosin Va to Rab27a. In this study, we show that Mlp is also a microtubule plus end–tracking protein or...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Xufeng S., Tsan, Grace L., Hammer, John A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171176/
https://www.ncbi.nlm.nih.gov/pubmed/16247022
http://dx.doi.org/10.1083/jcb.200503028
_version_ 1782144902264520704
author Wu, Xufeng S.
Tsan, Grace L.
Hammer, John A.
author_facet Wu, Xufeng S.
Tsan, Grace L.
Hammer, John A.
author_sort Wu, Xufeng S.
collection PubMed
description In mouse melanocytes, myosin Va is recruited onto the surface of melanosomes by a receptor complex containing Rab27a that is present in the melanosome membrane and melanophilin (Mlp), which links myosin Va to Rab27a. In this study, we show that Mlp is also a microtubule plus end–tracking protein or +TIP. Moreover, myosin Va tracks the plus end in a Mlp-dependent manner. Data showing that overexpression and short inhibitory RNA knockdown of the +TIP EB1 have opposite effects on Mlp–microtubule interaction, that Mlp interacts directly with EB1, and that deletion from Mlp of a region similar to one in the adenomatous polyposis coli protein involved in EB1 binding blocks Mlp's ability to plus end track argue that Mlp tracks the plus end directly by hitchhiking on EB1. These results identify a novel +TIP and indicate that vertebrate cells possess a +TIP complex that is similar to the Myo2p–Kar9p–Bim1p complex in yeast. We suggest that the +TIP complex identified in this study may serve to focus the transfer of melanosomes from microtubules to actin at the microtubule plus end.
format Text
id pubmed-2171176
institution National Center for Biotechnology Information
language English
publishDate 2005
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21711762008-03-05 Melanophilin and myosin Va track the microtubule plus end on EB1 Wu, Xufeng S. Tsan, Grace L. Hammer, John A. J Cell Biol Research Articles In mouse melanocytes, myosin Va is recruited onto the surface of melanosomes by a receptor complex containing Rab27a that is present in the melanosome membrane and melanophilin (Mlp), which links myosin Va to Rab27a. In this study, we show that Mlp is also a microtubule plus end–tracking protein or +TIP. Moreover, myosin Va tracks the plus end in a Mlp-dependent manner. Data showing that overexpression and short inhibitory RNA knockdown of the +TIP EB1 have opposite effects on Mlp–microtubule interaction, that Mlp interacts directly with EB1, and that deletion from Mlp of a region similar to one in the adenomatous polyposis coli protein involved in EB1 binding blocks Mlp's ability to plus end track argue that Mlp tracks the plus end directly by hitchhiking on EB1. These results identify a novel +TIP and indicate that vertebrate cells possess a +TIP complex that is similar to the Myo2p–Kar9p–Bim1p complex in yeast. We suggest that the +TIP complex identified in this study may serve to focus the transfer of melanosomes from microtubules to actin at the microtubule plus end. The Rockefeller University Press 2005-10-24 /pmc/articles/PMC2171176/ /pubmed/16247022 http://dx.doi.org/10.1083/jcb.200503028 Text en Copyright © 2005, Government This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Wu, Xufeng S.
Tsan, Grace L.
Hammer, John A.
Melanophilin and myosin Va track the microtubule plus end on EB1
title Melanophilin and myosin Va track the microtubule plus end on EB1
title_full Melanophilin and myosin Va track the microtubule plus end on EB1
title_fullStr Melanophilin and myosin Va track the microtubule plus end on EB1
title_full_unstemmed Melanophilin and myosin Va track the microtubule plus end on EB1
title_short Melanophilin and myosin Va track the microtubule plus end on EB1
title_sort melanophilin and myosin va track the microtubule plus end on eb1
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171176/
https://www.ncbi.nlm.nih.gov/pubmed/16247022
http://dx.doi.org/10.1083/jcb.200503028
work_keys_str_mv AT wuxufengs melanophilinandmyosinvatrackthemicrotubuleplusendoneb1
AT tsangracel melanophilinandmyosinvatrackthemicrotubuleplusendoneb1
AT hammerjohna melanophilinandmyosinvatrackthemicrotubuleplusendoneb1