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Mechanical force mobilizes zyxin from focal adhesions to actin filaments and regulates cytoskeletal reinforcement

Organs and tissues adapt to acute or chronic mechanical stress by remodeling their actin cytoskeletons. Cells that are stimulated by cyclic stretch or shear stress in vitro undergo bimodal cytoskeletal responses that include rapid reinforcement and gradual reorientation of actin stress fibers; howev...

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Detalles Bibliográficos
Autores principales: Yoshigi, Masaaki, Hoffman, Laura M., Jensen, Christopher C., Yost, H. Joseph, Beckerle, Mary C.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171187/
https://www.ncbi.nlm.nih.gov/pubmed/16247023
http://dx.doi.org/10.1083/jcb.200505018
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author Yoshigi, Masaaki
Hoffman, Laura M.
Jensen, Christopher C.
Yost, H. Joseph
Beckerle, Mary C.
author_facet Yoshigi, Masaaki
Hoffman, Laura M.
Jensen, Christopher C.
Yost, H. Joseph
Beckerle, Mary C.
author_sort Yoshigi, Masaaki
collection PubMed
description Organs and tissues adapt to acute or chronic mechanical stress by remodeling their actin cytoskeletons. Cells that are stimulated by cyclic stretch or shear stress in vitro undergo bimodal cytoskeletal responses that include rapid reinforcement and gradual reorientation of actin stress fibers; however, the mechanism by which cells respond to mechanical cues has been obscure. We report that the application of either unidirectional cyclic stretch or shear stress to cells results in robust mobilization of zyxin from focal adhesions to actin filaments, whereas many other focal adhesion proteins and zyxin family members remain at focal adhesions. Mechanical stress also induces the rapid zyxin-dependent mobilization of vasodilator-stimulated phosphoprotein from focal adhesions to actin filaments. Thickening of actin stress fibers reflects a cellular adaptation to mechanical stress; this cytoskeletal reinforcement coincides with zyxin mobilization and is abrogated in zyxin-null cells. Our findings identify zyxin as a mechanosensitive protein and provide mechanistic insight into how cells respond to mechanical cues.
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spelling pubmed-21711872008-03-05 Mechanical force mobilizes zyxin from focal adhesions to actin filaments and regulates cytoskeletal reinforcement Yoshigi, Masaaki Hoffman, Laura M. Jensen, Christopher C. Yost, H. Joseph Beckerle, Mary C. J Cell Biol Research Articles Organs and tissues adapt to acute or chronic mechanical stress by remodeling their actin cytoskeletons. Cells that are stimulated by cyclic stretch or shear stress in vitro undergo bimodal cytoskeletal responses that include rapid reinforcement and gradual reorientation of actin stress fibers; however, the mechanism by which cells respond to mechanical cues has been obscure. We report that the application of either unidirectional cyclic stretch or shear stress to cells results in robust mobilization of zyxin from focal adhesions to actin filaments, whereas many other focal adhesion proteins and zyxin family members remain at focal adhesions. Mechanical stress also induces the rapid zyxin-dependent mobilization of vasodilator-stimulated phosphoprotein from focal adhesions to actin filaments. Thickening of actin stress fibers reflects a cellular adaptation to mechanical stress; this cytoskeletal reinforcement coincides with zyxin mobilization and is abrogated in zyxin-null cells. Our findings identify zyxin as a mechanosensitive protein and provide mechanistic insight into how cells respond to mechanical cues. The Rockefeller University Press 2005-10-24 /pmc/articles/PMC2171187/ /pubmed/16247023 http://dx.doi.org/10.1083/jcb.200505018 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Yoshigi, Masaaki
Hoffman, Laura M.
Jensen, Christopher C.
Yost, H. Joseph
Beckerle, Mary C.
Mechanical force mobilizes zyxin from focal adhesions to actin filaments and regulates cytoskeletal reinforcement
title Mechanical force mobilizes zyxin from focal adhesions to actin filaments and regulates cytoskeletal reinforcement
title_full Mechanical force mobilizes zyxin from focal adhesions to actin filaments and regulates cytoskeletal reinforcement
title_fullStr Mechanical force mobilizes zyxin from focal adhesions to actin filaments and regulates cytoskeletal reinforcement
title_full_unstemmed Mechanical force mobilizes zyxin from focal adhesions to actin filaments and regulates cytoskeletal reinforcement
title_short Mechanical force mobilizes zyxin from focal adhesions to actin filaments and regulates cytoskeletal reinforcement
title_sort mechanical force mobilizes zyxin from focal adhesions to actin filaments and regulates cytoskeletal reinforcement
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171187/
https://www.ncbi.nlm.nih.gov/pubmed/16247023
http://dx.doi.org/10.1083/jcb.200505018
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