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Condensation of the plasma membrane at the site of T lymphocyte activation

After activation, T lymphocytes restructure their cell surface to form membrane domains at T cell receptor (TCR)–signaling foci and immunological synapses (ISs). To address whether these rearrangements involve alteration in the structure of the plasma membrane bilayer, we used the fluorescent probe...

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Autores principales: Gaus, Katharina, Chklovskaia, Elena, Fazekas de St. Groth, Barbara, Jessup, Wendy, Harder, Thomas
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171224/
https://www.ncbi.nlm.nih.gov/pubmed/16203859
http://dx.doi.org/10.1083/jcb.200505047
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author Gaus, Katharina
Chklovskaia, Elena
Fazekas de St. Groth, Barbara
Jessup, Wendy
Harder, Thomas
author_facet Gaus, Katharina
Chklovskaia, Elena
Fazekas de St. Groth, Barbara
Jessup, Wendy
Harder, Thomas
author_sort Gaus, Katharina
collection PubMed
description After activation, T lymphocytes restructure their cell surface to form membrane domains at T cell receptor (TCR)–signaling foci and immunological synapses (ISs). To address whether these rearrangements involve alteration in the structure of the plasma membrane bilayer, we used the fluorescent probe Laurdan to visualize its lipid order. We observed a condensation of the plasma membrane at TCR activation sites. The formation of ordered domains depends on the presence of the transmembrane protein linker for the activation of T cells and Src kinase activity. Moreover, these ordered domains are stabilized by the actin cytoskeleton. Membrane condensation occurs upon TCR stimulation alone but is prolonged by CD28 costimulation with TCR. In ISs, which are formed by conjugates of TCR transgenic T lymphocytes and cognate antigen-presenting cells, similar condensed membrane phases form first in central regions and later at the periphery of synapses. The formation of condensed membrane domains at T cell activation sites biophysically reflects membrane raft accumulation, which has potential implications for signaling at ISs.
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spelling pubmed-21712242008-03-05 Condensation of the plasma membrane at the site of T lymphocyte activation Gaus, Katharina Chklovskaia, Elena Fazekas de St. Groth, Barbara Jessup, Wendy Harder, Thomas J Cell Biol Research Articles After activation, T lymphocytes restructure their cell surface to form membrane domains at T cell receptor (TCR)–signaling foci and immunological synapses (ISs). To address whether these rearrangements involve alteration in the structure of the plasma membrane bilayer, we used the fluorescent probe Laurdan to visualize its lipid order. We observed a condensation of the plasma membrane at TCR activation sites. The formation of ordered domains depends on the presence of the transmembrane protein linker for the activation of T cells and Src kinase activity. Moreover, these ordered domains are stabilized by the actin cytoskeleton. Membrane condensation occurs upon TCR stimulation alone but is prolonged by CD28 costimulation with TCR. In ISs, which are formed by conjugates of TCR transgenic T lymphocytes and cognate antigen-presenting cells, similar condensed membrane phases form first in central regions and later at the periphery of synapses. The formation of condensed membrane domains at T cell activation sites biophysically reflects membrane raft accumulation, which has potential implications for signaling at ISs. The Rockefeller University Press 2005-10-10 /pmc/articles/PMC2171224/ /pubmed/16203859 http://dx.doi.org/10.1083/jcb.200505047 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Gaus, Katharina
Chklovskaia, Elena
Fazekas de St. Groth, Barbara
Jessup, Wendy
Harder, Thomas
Condensation of the plasma membrane at the site of T lymphocyte activation
title Condensation of the plasma membrane at the site of T lymphocyte activation
title_full Condensation of the plasma membrane at the site of T lymphocyte activation
title_fullStr Condensation of the plasma membrane at the site of T lymphocyte activation
title_full_unstemmed Condensation of the plasma membrane at the site of T lymphocyte activation
title_short Condensation of the plasma membrane at the site of T lymphocyte activation
title_sort condensation of the plasma membrane at the site of t lymphocyte activation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171224/
https://www.ncbi.nlm.nih.gov/pubmed/16203859
http://dx.doi.org/10.1083/jcb.200505047
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