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Condensation of the plasma membrane at the site of T lymphocyte activation
After activation, T lymphocytes restructure their cell surface to form membrane domains at T cell receptor (TCR)–signaling foci and immunological synapses (ISs). To address whether these rearrangements involve alteration in the structure of the plasma membrane bilayer, we used the fluorescent probe...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171224/ https://www.ncbi.nlm.nih.gov/pubmed/16203859 http://dx.doi.org/10.1083/jcb.200505047 |
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author | Gaus, Katharina Chklovskaia, Elena Fazekas de St. Groth, Barbara Jessup, Wendy Harder, Thomas |
author_facet | Gaus, Katharina Chklovskaia, Elena Fazekas de St. Groth, Barbara Jessup, Wendy Harder, Thomas |
author_sort | Gaus, Katharina |
collection | PubMed |
description | After activation, T lymphocytes restructure their cell surface to form membrane domains at T cell receptor (TCR)–signaling foci and immunological synapses (ISs). To address whether these rearrangements involve alteration in the structure of the plasma membrane bilayer, we used the fluorescent probe Laurdan to visualize its lipid order. We observed a condensation of the plasma membrane at TCR activation sites. The formation of ordered domains depends on the presence of the transmembrane protein linker for the activation of T cells and Src kinase activity. Moreover, these ordered domains are stabilized by the actin cytoskeleton. Membrane condensation occurs upon TCR stimulation alone but is prolonged by CD28 costimulation with TCR. In ISs, which are formed by conjugates of TCR transgenic T lymphocytes and cognate antigen-presenting cells, similar condensed membrane phases form first in central regions and later at the periphery of synapses. The formation of condensed membrane domains at T cell activation sites biophysically reflects membrane raft accumulation, which has potential implications for signaling at ISs. |
format | Text |
id | pubmed-2171224 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21712242008-03-05 Condensation of the plasma membrane at the site of T lymphocyte activation Gaus, Katharina Chklovskaia, Elena Fazekas de St. Groth, Barbara Jessup, Wendy Harder, Thomas J Cell Biol Research Articles After activation, T lymphocytes restructure their cell surface to form membrane domains at T cell receptor (TCR)–signaling foci and immunological synapses (ISs). To address whether these rearrangements involve alteration in the structure of the plasma membrane bilayer, we used the fluorescent probe Laurdan to visualize its lipid order. We observed a condensation of the plasma membrane at TCR activation sites. The formation of ordered domains depends on the presence of the transmembrane protein linker for the activation of T cells and Src kinase activity. Moreover, these ordered domains are stabilized by the actin cytoskeleton. Membrane condensation occurs upon TCR stimulation alone but is prolonged by CD28 costimulation with TCR. In ISs, which are formed by conjugates of TCR transgenic T lymphocytes and cognate antigen-presenting cells, similar condensed membrane phases form first in central regions and later at the periphery of synapses. The formation of condensed membrane domains at T cell activation sites biophysically reflects membrane raft accumulation, which has potential implications for signaling at ISs. The Rockefeller University Press 2005-10-10 /pmc/articles/PMC2171224/ /pubmed/16203859 http://dx.doi.org/10.1083/jcb.200505047 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Gaus, Katharina Chklovskaia, Elena Fazekas de St. Groth, Barbara Jessup, Wendy Harder, Thomas Condensation of the plasma membrane at the site of T lymphocyte activation |
title | Condensation of the plasma membrane at the site of T lymphocyte activation |
title_full | Condensation of the plasma membrane at the site of T lymphocyte activation |
title_fullStr | Condensation of the plasma membrane at the site of T lymphocyte activation |
title_full_unstemmed | Condensation of the plasma membrane at the site of T lymphocyte activation |
title_short | Condensation of the plasma membrane at the site of T lymphocyte activation |
title_sort | condensation of the plasma membrane at the site of t lymphocyte activation |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171224/ https://www.ncbi.nlm.nih.gov/pubmed/16203859 http://dx.doi.org/10.1083/jcb.200505047 |
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