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IGF-I increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors

Muscle damage has been shown to enhance the contribution of bone marrow–derived cells (BMDCs) to regenerating skeletal muscle. One responsible cell type involved in this process is a hematopoietic stem cell derivative, the myelomonocytic precursor (MMC). However, the molecular components responsible...

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Autores principales: Sacco, Alessandra, Doyonnas, Regis, LaBarge, Mark A., Hammer, Mark M., Kraft, Peggy, Blau, Helen M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171272/
https://www.ncbi.nlm.nih.gov/pubmed/16275752
http://dx.doi.org/10.1083/jcb.200506123
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author Sacco, Alessandra
Doyonnas, Regis
LaBarge, Mark A.
Hammer, Mark M.
Kraft, Peggy
Blau, Helen M.
author_facet Sacco, Alessandra
Doyonnas, Regis
LaBarge, Mark A.
Hammer, Mark M.
Kraft, Peggy
Blau, Helen M.
author_sort Sacco, Alessandra
collection PubMed
description Muscle damage has been shown to enhance the contribution of bone marrow–derived cells (BMDCs) to regenerating skeletal muscle. One responsible cell type involved in this process is a hematopoietic stem cell derivative, the myelomonocytic precursor (MMC). However, the molecular components responsible for this injury-related response remain largely unknown. In this paper, we show that delivery of insulin-like growth factor I (IGF-I) to adult skeletal muscle by three different methods—plasmid electroporation, injection of genetically engineered myoblasts, and recombinant protein injection—increases the integration of BMDCs up to fourfold. To investigate the underlying mechanism, we developed an in vitro fusion assay in which co-cultures of MMCs and myotubes were exposed to IGF-I. The number of fusion events was substantially augmented by IGF-I, independent of its effect on cell survival. These results provide novel evidence that a single factor, IGF-I, is sufficient to enhance the fusion of bone marrow derivatives with adult skeletal muscle.
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spelling pubmed-21712722008-03-05 IGF-I increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors Sacco, Alessandra Doyonnas, Regis LaBarge, Mark A. Hammer, Mark M. Kraft, Peggy Blau, Helen M. J Cell Biol Research Articles Muscle damage has been shown to enhance the contribution of bone marrow–derived cells (BMDCs) to regenerating skeletal muscle. One responsible cell type involved in this process is a hematopoietic stem cell derivative, the myelomonocytic precursor (MMC). However, the molecular components responsible for this injury-related response remain largely unknown. In this paper, we show that delivery of insulin-like growth factor I (IGF-I) to adult skeletal muscle by three different methods—plasmid electroporation, injection of genetically engineered myoblasts, and recombinant protein injection—increases the integration of BMDCs up to fourfold. To investigate the underlying mechanism, we developed an in vitro fusion assay in which co-cultures of MMCs and myotubes were exposed to IGF-I. The number of fusion events was substantially augmented by IGF-I, independent of its effect on cell survival. These results provide novel evidence that a single factor, IGF-I, is sufficient to enhance the fusion of bone marrow derivatives with adult skeletal muscle. The Rockefeller University Press 2005-11-07 /pmc/articles/PMC2171272/ /pubmed/16275752 http://dx.doi.org/10.1083/jcb.200506123 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Sacco, Alessandra
Doyonnas, Regis
LaBarge, Mark A.
Hammer, Mark M.
Kraft, Peggy
Blau, Helen M.
IGF-I increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors
title IGF-I increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors
title_full IGF-I increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors
title_fullStr IGF-I increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors
title_full_unstemmed IGF-I increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors
title_short IGF-I increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors
title_sort igf-i increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171272/
https://www.ncbi.nlm.nih.gov/pubmed/16275752
http://dx.doi.org/10.1083/jcb.200506123
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