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Influences on neural lineage and mode of division in the zebrafish retina in vivo
Cell determination in the retina has been under intense investigation since the discovery that retinal progenitors generate clones of apparently random composition (Price, J., D. Turner, and C. Cepko. 1987. Proc. Natl. Acad. Sci. USA. 84:156–160; Holt, C.E., T.W. Bertsch, H.M. Ellis, and W.A. Harris...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2005
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171316/ https://www.ncbi.nlm.nih.gov/pubmed/16365165 http://dx.doi.org/10.1083/jcb.200509098 |
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author | Poggi, Lucia Vitorino, Marta Masai, Ichiro Harris, William A. |
author_facet | Poggi, Lucia Vitorino, Marta Masai, Ichiro Harris, William A. |
author_sort | Poggi, Lucia |
collection | PubMed |
description | Cell determination in the retina has been under intense investigation since the discovery that retinal progenitors generate clones of apparently random composition (Price, J., D. Turner, and C. Cepko. 1987. Proc. Natl. Acad. Sci. USA. 84:156–160; Holt, C.E., T.W. Bertsch, H.M. Ellis, and W.A. Harris. 1988. Neuron. 1:15–26; Wetts, R., and S.E. Fraser. 1988. Science. 239:1142–1145). Examination of fixed tissue, however, sheds little light on lineage patterns or on the relationship between the orientation of division and cell fate. In this study, three-dimensional time-lapse analyses were used to trace lineages of retinal progenitors expressing green fluorescent protein under the control of the ath5 promoter. Surprisingly, these cells divide just once along the circumferential axis to produce two postmitotic daughters, one of which becomes a retinal ganglion cell (RGC). Interestingly, when these same progenitors are transplanted into a mutant environment lacking RGCs, they often divide along the central-peripheral axis and produce two RGCs. This study provides the first insight into reproducible lineage patterns of retinal progenitors in vivo and the first evidence that environmental signals influence the orientation of cell division and the lineage of neural progenitors. |
format | Text |
id | pubmed-2171316 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21713162008-03-05 Influences on neural lineage and mode of division in the zebrafish retina in vivo Poggi, Lucia Vitorino, Marta Masai, Ichiro Harris, William A. J Cell Biol Research Articles Cell determination in the retina has been under intense investigation since the discovery that retinal progenitors generate clones of apparently random composition (Price, J., D. Turner, and C. Cepko. 1987. Proc. Natl. Acad. Sci. USA. 84:156–160; Holt, C.E., T.W. Bertsch, H.M. Ellis, and W.A. Harris. 1988. Neuron. 1:15–26; Wetts, R., and S.E. Fraser. 1988. Science. 239:1142–1145). Examination of fixed tissue, however, sheds little light on lineage patterns or on the relationship between the orientation of division and cell fate. In this study, three-dimensional time-lapse analyses were used to trace lineages of retinal progenitors expressing green fluorescent protein under the control of the ath5 promoter. Surprisingly, these cells divide just once along the circumferential axis to produce two postmitotic daughters, one of which becomes a retinal ganglion cell (RGC). Interestingly, when these same progenitors are transplanted into a mutant environment lacking RGCs, they often divide along the central-peripheral axis and produce two RGCs. This study provides the first insight into reproducible lineage patterns of retinal progenitors in vivo and the first evidence that environmental signals influence the orientation of cell division and the lineage of neural progenitors. The Rockefeller University Press 2005-12-19 /pmc/articles/PMC2171316/ /pubmed/16365165 http://dx.doi.org/10.1083/jcb.200509098 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Poggi, Lucia Vitorino, Marta Masai, Ichiro Harris, William A. Influences on neural lineage and mode of division in the zebrafish retina in vivo |
title | Influences on neural lineage and mode of division in the zebrafish retina in vivo |
title_full | Influences on neural lineage and mode of division in the zebrafish retina in vivo |
title_fullStr | Influences on neural lineage and mode of division in the zebrafish retina in vivo |
title_full_unstemmed | Influences on neural lineage and mode of division in the zebrafish retina in vivo |
title_short | Influences on neural lineage and mode of division in the zebrafish retina in vivo |
title_sort | influences on neural lineage and mode of division in the zebrafish retina in vivo |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171316/ https://www.ncbi.nlm.nih.gov/pubmed/16365165 http://dx.doi.org/10.1083/jcb.200509098 |
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