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Influences on neural lineage and mode of division in the zebrafish retina in vivo

Cell determination in the retina has been under intense investigation since the discovery that retinal progenitors generate clones of apparently random composition (Price, J., D. Turner, and C. Cepko. 1987. Proc. Natl. Acad. Sci. USA. 84:156–160; Holt, C.E., T.W. Bertsch, H.M. Ellis, and W.A. Harris...

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Detalles Bibliográficos
Autores principales: Poggi, Lucia, Vitorino, Marta, Masai, Ichiro, Harris, William A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171316/
https://www.ncbi.nlm.nih.gov/pubmed/16365165
http://dx.doi.org/10.1083/jcb.200509098
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author Poggi, Lucia
Vitorino, Marta
Masai, Ichiro
Harris, William A.
author_facet Poggi, Lucia
Vitorino, Marta
Masai, Ichiro
Harris, William A.
author_sort Poggi, Lucia
collection PubMed
description Cell determination in the retina has been under intense investigation since the discovery that retinal progenitors generate clones of apparently random composition (Price, J., D. Turner, and C. Cepko. 1987. Proc. Natl. Acad. Sci. USA. 84:156–160; Holt, C.E., T.W. Bertsch, H.M. Ellis, and W.A. Harris. 1988. Neuron. 1:15–26; Wetts, R., and S.E. Fraser. 1988. Science. 239:1142–1145). Examination of fixed tissue, however, sheds little light on lineage patterns or on the relationship between the orientation of division and cell fate. In this study, three-dimensional time-lapse analyses were used to trace lineages of retinal progenitors expressing green fluorescent protein under the control of the ath5 promoter. Surprisingly, these cells divide just once along the circumferential axis to produce two postmitotic daughters, one of which becomes a retinal ganglion cell (RGC). Interestingly, when these same progenitors are transplanted into a mutant environment lacking RGCs, they often divide along the central-peripheral axis and produce two RGCs. This study provides the first insight into reproducible lineage patterns of retinal progenitors in vivo and the first evidence that environmental signals influence the orientation of cell division and the lineage of neural progenitors.
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spelling pubmed-21713162008-03-05 Influences on neural lineage and mode of division in the zebrafish retina in vivo Poggi, Lucia Vitorino, Marta Masai, Ichiro Harris, William A. J Cell Biol Research Articles Cell determination in the retina has been under intense investigation since the discovery that retinal progenitors generate clones of apparently random composition (Price, J., D. Turner, and C. Cepko. 1987. Proc. Natl. Acad. Sci. USA. 84:156–160; Holt, C.E., T.W. Bertsch, H.M. Ellis, and W.A. Harris. 1988. Neuron. 1:15–26; Wetts, R., and S.E. Fraser. 1988. Science. 239:1142–1145). Examination of fixed tissue, however, sheds little light on lineage patterns or on the relationship between the orientation of division and cell fate. In this study, three-dimensional time-lapse analyses were used to trace lineages of retinal progenitors expressing green fluorescent protein under the control of the ath5 promoter. Surprisingly, these cells divide just once along the circumferential axis to produce two postmitotic daughters, one of which becomes a retinal ganglion cell (RGC). Interestingly, when these same progenitors are transplanted into a mutant environment lacking RGCs, they often divide along the central-peripheral axis and produce two RGCs. This study provides the first insight into reproducible lineage patterns of retinal progenitors in vivo and the first evidence that environmental signals influence the orientation of cell division and the lineage of neural progenitors. The Rockefeller University Press 2005-12-19 /pmc/articles/PMC2171316/ /pubmed/16365165 http://dx.doi.org/10.1083/jcb.200509098 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Poggi, Lucia
Vitorino, Marta
Masai, Ichiro
Harris, William A.
Influences on neural lineage and mode of division in the zebrafish retina in vivo
title Influences on neural lineage and mode of division in the zebrafish retina in vivo
title_full Influences on neural lineage and mode of division in the zebrafish retina in vivo
title_fullStr Influences on neural lineage and mode of division in the zebrafish retina in vivo
title_full_unstemmed Influences on neural lineage and mode of division in the zebrafish retina in vivo
title_short Influences on neural lineage and mode of division in the zebrafish retina in vivo
title_sort influences on neural lineage and mode of division in the zebrafish retina in vivo
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171316/
https://www.ncbi.nlm.nih.gov/pubmed/16365165
http://dx.doi.org/10.1083/jcb.200509098
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