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AtRMR1 functions as a cargo receptor for protein trafficking to the protein storage vacuole

Organellar proteins are sorted by cargo receptors on the way to their final destination. However, receptors for proteins that are destined for the protein storage vacuole (PSV) are largely unknown. In this study, we investigated the biological role that Arabidopsis thaliana receptor homology region...

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Detalles Bibliográficos
Autores principales: Park, Misoon, Lee, Daeseok, Lee, Gil-Je, Hwang, Inhwan
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171354/
https://www.ncbi.nlm.nih.gov/pubmed/16115960
http://dx.doi.org/10.1083/jcb.200504112
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author Park, Misoon
Lee, Daeseok
Lee, Gil-Je
Hwang, Inhwan
author_facet Park, Misoon
Lee, Daeseok
Lee, Gil-Je
Hwang, Inhwan
author_sort Park, Misoon
collection PubMed
description Organellar proteins are sorted by cargo receptors on the way to their final destination. However, receptors for proteins that are destined for the protein storage vacuole (PSV) are largely unknown. In this study, we investigated the biological role that Arabidopsis thaliana receptor homology region transmembrane domain ring H2 motif protein (AtRMR) 1 plays in protein trafficking to the PSV. AtRMR1 mainly colocalized to the prevacuolar compartment of the PSV, but a minor portion also localized to the Golgi complex. The coexpression of AtRMR1 mutants that were localized to the Golgi complex strongly inhibited the trafficking of phaseolin to the PSV and caused accumulation of phaseolin in the Golgi complex or its secretion. Coimmunoprecipitation and in vitro binding assays revealed that the lumenal domain of AtRMR1 interacts with the COOH-terminal sorting signal of phaseolin at acidic pH. Furthermore, phaseolin colocalized with AtRMR1 on its way to the PSV. Based on these results, we propose that AtRMR1 functions as the sorting receptor of phaseolin for its trafficking to the PSV.
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spelling pubmed-21713542008-03-05 AtRMR1 functions as a cargo receptor for protein trafficking to the protein storage vacuole Park, Misoon Lee, Daeseok Lee, Gil-Je Hwang, Inhwan J Cell Biol Research Articles Organellar proteins are sorted by cargo receptors on the way to their final destination. However, receptors for proteins that are destined for the protein storage vacuole (PSV) are largely unknown. In this study, we investigated the biological role that Arabidopsis thaliana receptor homology region transmembrane domain ring H2 motif protein (AtRMR) 1 plays in protein trafficking to the PSV. AtRMR1 mainly colocalized to the prevacuolar compartment of the PSV, but a minor portion also localized to the Golgi complex. The coexpression of AtRMR1 mutants that were localized to the Golgi complex strongly inhibited the trafficking of phaseolin to the PSV and caused accumulation of phaseolin in the Golgi complex or its secretion. Coimmunoprecipitation and in vitro binding assays revealed that the lumenal domain of AtRMR1 interacts with the COOH-terminal sorting signal of phaseolin at acidic pH. Furthermore, phaseolin colocalized with AtRMR1 on its way to the PSV. Based on these results, we propose that AtRMR1 functions as the sorting receptor of phaseolin for its trafficking to the PSV. The Rockefeller University Press 2005-08-29 /pmc/articles/PMC2171354/ /pubmed/16115960 http://dx.doi.org/10.1083/jcb.200504112 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Park, Misoon
Lee, Daeseok
Lee, Gil-Je
Hwang, Inhwan
AtRMR1 functions as a cargo receptor for protein trafficking to the protein storage vacuole
title AtRMR1 functions as a cargo receptor for protein trafficking to the protein storage vacuole
title_full AtRMR1 functions as a cargo receptor for protein trafficking to the protein storage vacuole
title_fullStr AtRMR1 functions as a cargo receptor for protein trafficking to the protein storage vacuole
title_full_unstemmed AtRMR1 functions as a cargo receptor for protein trafficking to the protein storage vacuole
title_short AtRMR1 functions as a cargo receptor for protein trafficking to the protein storage vacuole
title_sort atrmr1 functions as a cargo receptor for protein trafficking to the protein storage vacuole
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171354/
https://www.ncbi.nlm.nih.gov/pubmed/16115960
http://dx.doi.org/10.1083/jcb.200504112
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