SNAREs can promote complete fusion and hemifusion as alternative outcomes

Using a cell fusion assay, we show here that in addition to complete fusion SNAREs also promote hemifusion as an alternative outcome. Approximately 65% of events resulted in full fusion, and the remaining 35% in hemifusion; of those, approximately two thirds were permanent and approximately one thir...

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Detalles Bibliográficos
Autores principales: Giraudo, Claudio G., Hu, Chuan, You, Daoqi, Slovic, Avram M., Mosharov, Eugene V., Sulzer, David, Melia, Thomas J., Rothman, James E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171417/
https://www.ncbi.nlm.nih.gov/pubmed/16027221
http://dx.doi.org/10.1083/jcb.200501093
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author Giraudo, Claudio G.
Hu, Chuan
You, Daoqi
Slovic, Avram M.
Mosharov, Eugene V.
Sulzer, David
Melia, Thomas J.
Rothman, James E.
author_facet Giraudo, Claudio G.
Hu, Chuan
You, Daoqi
Slovic, Avram M.
Mosharov, Eugene V.
Sulzer, David
Melia, Thomas J.
Rothman, James E.
author_sort Giraudo, Claudio G.
collection PubMed
description Using a cell fusion assay, we show here that in addition to complete fusion SNAREs also promote hemifusion as an alternative outcome. Approximately 65% of events resulted in full fusion, and the remaining 35% in hemifusion; of those, approximately two thirds were permanent and approximately one third were reversible. We predict that this relatively close balance among outcomes could be tipped by binding of regulatory proteins to the SNAREs, allowing for dynamic physiological regulation between full fusion and reversible kiss-and-run–like events.
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spelling pubmed-21714172008-03-05 SNAREs can promote complete fusion and hemifusion as alternative outcomes Giraudo, Claudio G. Hu, Chuan You, Daoqi Slovic, Avram M. Mosharov, Eugene V. Sulzer, David Melia, Thomas J. Rothman, James E. J Cell Biol Research Articles Using a cell fusion assay, we show here that in addition to complete fusion SNAREs also promote hemifusion as an alternative outcome. Approximately 65% of events resulted in full fusion, and the remaining 35% in hemifusion; of those, approximately two thirds were permanent and approximately one third were reversible. We predict that this relatively close balance among outcomes could be tipped by binding of regulatory proteins to the SNAREs, allowing for dynamic physiological regulation between full fusion and reversible kiss-and-run–like events. The Rockefeller University Press 2005-07-18 /pmc/articles/PMC2171417/ /pubmed/16027221 http://dx.doi.org/10.1083/jcb.200501093 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Giraudo, Claudio G.
Hu, Chuan
You, Daoqi
Slovic, Avram M.
Mosharov, Eugene V.
Sulzer, David
Melia, Thomas J.
Rothman, James E.
SNAREs can promote complete fusion and hemifusion as alternative outcomes
title SNAREs can promote complete fusion and hemifusion as alternative outcomes
title_full SNAREs can promote complete fusion and hemifusion as alternative outcomes
title_fullStr SNAREs can promote complete fusion and hemifusion as alternative outcomes
title_full_unstemmed SNAREs can promote complete fusion and hemifusion as alternative outcomes
title_short SNAREs can promote complete fusion and hemifusion as alternative outcomes
title_sort snares can promote complete fusion and hemifusion as alternative outcomes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171417/
https://www.ncbi.nlm.nih.gov/pubmed/16027221
http://dx.doi.org/10.1083/jcb.200501093
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