Cdc42 and Par6–PKCζ regulate the spatially localized association of Dlg1 and APC to control cell polarization

Cell polarization is essential in a wide range of biological processes such as morphogenesis, asymmetric division, and directed migration. In this study, we show that two tumor suppressor proteins, adenomatous polyposis coli (APC) and Dlg1-SAP97, are required for the polarization of migrating astroc...

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Autores principales: Etienne-Manneville, Sandrine, Manneville, Jean-Baptiste, Nicholls, Sarah, Ferenczi, Michael A., Hall, Alan
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171429/
https://www.ncbi.nlm.nih.gov/pubmed/16157700
http://dx.doi.org/10.1083/jcb.200412172
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author Etienne-Manneville, Sandrine
Manneville, Jean-Baptiste
Nicholls, Sarah
Ferenczi, Michael A.
Hall, Alan
author_facet Etienne-Manneville, Sandrine
Manneville, Jean-Baptiste
Nicholls, Sarah
Ferenczi, Michael A.
Hall, Alan
author_sort Etienne-Manneville, Sandrine
collection PubMed
description Cell polarization is essential in a wide range of biological processes such as morphogenesis, asymmetric division, and directed migration. In this study, we show that two tumor suppressor proteins, adenomatous polyposis coli (APC) and Dlg1-SAP97, are required for the polarization of migrating astrocytes. Activation of the Par6–PKCζ complex by Cdc42 at the leading edge of migrating cells promotes both the localized association of APC with microtubule plus ends and the assembly of Dlg-containing puncta in the plasma membrane. Biochemical analysis and total internal reflection fluorescence microscopy reveal that the subsequent physical interaction between APC and Dlg1 is required for polarization of the microtubule cytoskeleton.
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spelling pubmed-21714292008-03-05 Cdc42 and Par6–PKCζ regulate the spatially localized association of Dlg1 and APC to control cell polarization Etienne-Manneville, Sandrine Manneville, Jean-Baptiste Nicholls, Sarah Ferenczi, Michael A. Hall, Alan J Cell Biol Research Articles Cell polarization is essential in a wide range of biological processes such as morphogenesis, asymmetric division, and directed migration. In this study, we show that two tumor suppressor proteins, adenomatous polyposis coli (APC) and Dlg1-SAP97, are required for the polarization of migrating astrocytes. Activation of the Par6–PKCζ complex by Cdc42 at the leading edge of migrating cells promotes both the localized association of APC with microtubule plus ends and the assembly of Dlg-containing puncta in the plasma membrane. Biochemical analysis and total internal reflection fluorescence microscopy reveal that the subsequent physical interaction between APC and Dlg1 is required for polarization of the microtubule cytoskeleton. The Rockefeller University Press 2005-09-12 /pmc/articles/PMC2171429/ /pubmed/16157700 http://dx.doi.org/10.1083/jcb.200412172 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Etienne-Manneville, Sandrine
Manneville, Jean-Baptiste
Nicholls, Sarah
Ferenczi, Michael A.
Hall, Alan
Cdc42 and Par6–PKCζ regulate the spatially localized association of Dlg1 and APC to control cell polarization
title Cdc42 and Par6–PKCζ regulate the spatially localized association of Dlg1 and APC to control cell polarization
title_full Cdc42 and Par6–PKCζ regulate the spatially localized association of Dlg1 and APC to control cell polarization
title_fullStr Cdc42 and Par6–PKCζ regulate the spatially localized association of Dlg1 and APC to control cell polarization
title_full_unstemmed Cdc42 and Par6–PKCζ regulate the spatially localized association of Dlg1 and APC to control cell polarization
title_short Cdc42 and Par6–PKCζ regulate the spatially localized association of Dlg1 and APC to control cell polarization
title_sort cdc42 and par6–pkcζ regulate the spatially localized association of dlg1 and apc to control cell polarization
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171429/
https://www.ncbi.nlm.nih.gov/pubmed/16157700
http://dx.doi.org/10.1083/jcb.200412172
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