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The origin recognition core complex regulates dendrite and spine development in postmitotic neurons
The origin recognition complex (ORC) ensures exactly one round of genome replication per cell cycle through acting as a molecular switch that precisely controls the assembly, firing, and inactivation of the replication initiation machinery. Recent data indicate that it may also coordinate the proces...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2005
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171496/ https://www.ncbi.nlm.nih.gov/pubmed/16087709 http://dx.doi.org/10.1083/jcb.200505075 |
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author | Huang, Zhen Zang, Keling Reichardt, Louis F. |
author_facet | Huang, Zhen Zang, Keling Reichardt, Louis F. |
author_sort | Huang, Zhen |
collection | PubMed |
description | The origin recognition complex (ORC) ensures exactly one round of genome replication per cell cycle through acting as a molecular switch that precisely controls the assembly, firing, and inactivation of the replication initiation machinery. Recent data indicate that it may also coordinate the processes of mitosis and cytokinesis and ensure the proper distribution of replicated genome to daughter cells. We have found that the ORC core subunits are highly expressed in the nervous system. They are selectively localized to the neuronal somatodendritic compartment and enriched in the membrane fraction. siRNA knockdown of ORC subunits dramatically reduced dendritic branch formation and severely impeded dendritic spine emergence. Expression of ORC ATPase motif mutants enhanced the branching of dendritic arbors. The ORC core complex thus appears to have a novel role in regulating dendrite and dendritic spine development in postmitotic neurons. |
format | Text |
id | pubmed-2171496 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21714962008-03-05 The origin recognition core complex regulates dendrite and spine development in postmitotic neurons Huang, Zhen Zang, Keling Reichardt, Louis F. J Cell Biol Research Articles The origin recognition complex (ORC) ensures exactly one round of genome replication per cell cycle through acting as a molecular switch that precisely controls the assembly, firing, and inactivation of the replication initiation machinery. Recent data indicate that it may also coordinate the processes of mitosis and cytokinesis and ensure the proper distribution of replicated genome to daughter cells. We have found that the ORC core subunits are highly expressed in the nervous system. They are selectively localized to the neuronal somatodendritic compartment and enriched in the membrane fraction. siRNA knockdown of ORC subunits dramatically reduced dendritic branch formation and severely impeded dendritic spine emergence. Expression of ORC ATPase motif mutants enhanced the branching of dendritic arbors. The ORC core complex thus appears to have a novel role in regulating dendrite and dendritic spine development in postmitotic neurons. The Rockefeller University Press 2005-08-15 /pmc/articles/PMC2171496/ /pubmed/16087709 http://dx.doi.org/10.1083/jcb.200505075 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Huang, Zhen Zang, Keling Reichardt, Louis F. The origin recognition core complex regulates dendrite and spine development in postmitotic neurons |
title | The origin recognition core complex regulates dendrite and spine development in postmitotic neurons |
title_full | The origin recognition core complex regulates dendrite and spine development in postmitotic neurons |
title_fullStr | The origin recognition core complex regulates dendrite and spine development in postmitotic neurons |
title_full_unstemmed | The origin recognition core complex regulates dendrite and spine development in postmitotic neurons |
title_short | The origin recognition core complex regulates dendrite and spine development in postmitotic neurons |
title_sort | origin recognition core complex regulates dendrite and spine development in postmitotic neurons |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171496/ https://www.ncbi.nlm.nih.gov/pubmed/16087709 http://dx.doi.org/10.1083/jcb.200505075 |
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