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The origin recognition core complex regulates dendrite and spine development in postmitotic neurons

The origin recognition complex (ORC) ensures exactly one round of genome replication per cell cycle through acting as a molecular switch that precisely controls the assembly, firing, and inactivation of the replication initiation machinery. Recent data indicate that it may also coordinate the proces...

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Detalles Bibliográficos
Autores principales: Huang, Zhen, Zang, Keling, Reichardt, Louis F.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171496/
https://www.ncbi.nlm.nih.gov/pubmed/16087709
http://dx.doi.org/10.1083/jcb.200505075
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author Huang, Zhen
Zang, Keling
Reichardt, Louis F.
author_facet Huang, Zhen
Zang, Keling
Reichardt, Louis F.
author_sort Huang, Zhen
collection PubMed
description The origin recognition complex (ORC) ensures exactly one round of genome replication per cell cycle through acting as a molecular switch that precisely controls the assembly, firing, and inactivation of the replication initiation machinery. Recent data indicate that it may also coordinate the processes of mitosis and cytokinesis and ensure the proper distribution of replicated genome to daughter cells. We have found that the ORC core subunits are highly expressed in the nervous system. They are selectively localized to the neuronal somatodendritic compartment and enriched in the membrane fraction. siRNA knockdown of ORC subunits dramatically reduced dendritic branch formation and severely impeded dendritic spine emergence. Expression of ORC ATPase motif mutants enhanced the branching of dendritic arbors. The ORC core complex thus appears to have a novel role in regulating dendrite and dendritic spine development in postmitotic neurons.
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spelling pubmed-21714962008-03-05 The origin recognition core complex regulates dendrite and spine development in postmitotic neurons Huang, Zhen Zang, Keling Reichardt, Louis F. J Cell Biol Research Articles The origin recognition complex (ORC) ensures exactly one round of genome replication per cell cycle through acting as a molecular switch that precisely controls the assembly, firing, and inactivation of the replication initiation machinery. Recent data indicate that it may also coordinate the processes of mitosis and cytokinesis and ensure the proper distribution of replicated genome to daughter cells. We have found that the ORC core subunits are highly expressed in the nervous system. They are selectively localized to the neuronal somatodendritic compartment and enriched in the membrane fraction. siRNA knockdown of ORC subunits dramatically reduced dendritic branch formation and severely impeded dendritic spine emergence. Expression of ORC ATPase motif mutants enhanced the branching of dendritic arbors. The ORC core complex thus appears to have a novel role in regulating dendrite and dendritic spine development in postmitotic neurons. The Rockefeller University Press 2005-08-15 /pmc/articles/PMC2171496/ /pubmed/16087709 http://dx.doi.org/10.1083/jcb.200505075 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Huang, Zhen
Zang, Keling
Reichardt, Louis F.
The origin recognition core complex regulates dendrite and spine development in postmitotic neurons
title The origin recognition core complex regulates dendrite and spine development in postmitotic neurons
title_full The origin recognition core complex regulates dendrite and spine development in postmitotic neurons
title_fullStr The origin recognition core complex regulates dendrite and spine development in postmitotic neurons
title_full_unstemmed The origin recognition core complex regulates dendrite and spine development in postmitotic neurons
title_short The origin recognition core complex regulates dendrite and spine development in postmitotic neurons
title_sort origin recognition core complex regulates dendrite and spine development in postmitotic neurons
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171496/
https://www.ncbi.nlm.nih.gov/pubmed/16087709
http://dx.doi.org/10.1083/jcb.200505075
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