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FAPP2 is involved in the transport of apical cargo in polarized MDCK cells

Phosphatidylinositol-4-phosphate (PI(4)P) is the main phosphoinositide in the Golgi complex and has been reported to play a pleiotropic role in transport of cargo from the trans-Golgi network to the plasma membrane (PM) in polarized Madin–Darby canine kidney (MDCK) cells. Overexpression of the chime...

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Autores principales: Vieira, Otilia V., Verkade, Paul, Manninen, Aki, Simons, Kai
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171512/
https://www.ncbi.nlm.nih.gov/pubmed/16103222
http://dx.doi.org/10.1083/jcb.200503078
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author Vieira, Otilia V.
Verkade, Paul
Manninen, Aki
Simons, Kai
author_facet Vieira, Otilia V.
Verkade, Paul
Manninen, Aki
Simons, Kai
author_sort Vieira, Otilia V.
collection PubMed
description Phosphatidylinositol-4-phosphate (PI(4)P) is the main phosphoinositide in the Golgi complex and has been reported to play a pleiotropic role in transport of cargo from the trans-Golgi network to the plasma membrane (PM) in polarized Madin–Darby canine kidney (MDCK) cells. Overexpression of the chimeric fluorescent protein encoding the pleckstrin homology domain, which is specific for PI(4)P, inhibited both apical and basolateral transport pathways. The transport of apical cargo from the Golgi was shown to be specifically decreased by adenovirus-mediated RNA interference directed against PI(4)P adaptor protein (FAPP) 2. FAPP1 depletion had no effect on transport. On the other hand, FAPP2 was not involved in the Golgi-to-PM transport of cargo that was targeted to the basolateral membrane domain. Thus, we conclude that FAPP2 plays a specific role in apical transport in MDCK cells.
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spelling pubmed-21715122008-03-05 FAPP2 is involved in the transport of apical cargo in polarized MDCK cells Vieira, Otilia V. Verkade, Paul Manninen, Aki Simons, Kai J Cell Biol Research Articles Phosphatidylinositol-4-phosphate (PI(4)P) is the main phosphoinositide in the Golgi complex and has been reported to play a pleiotropic role in transport of cargo from the trans-Golgi network to the plasma membrane (PM) in polarized Madin–Darby canine kidney (MDCK) cells. Overexpression of the chimeric fluorescent protein encoding the pleckstrin homology domain, which is specific for PI(4)P, inhibited both apical and basolateral transport pathways. The transport of apical cargo from the Golgi was shown to be specifically decreased by adenovirus-mediated RNA interference directed against PI(4)P adaptor protein (FAPP) 2. FAPP1 depletion had no effect on transport. On the other hand, FAPP2 was not involved in the Golgi-to-PM transport of cargo that was targeted to the basolateral membrane domain. Thus, we conclude that FAPP2 plays a specific role in apical transport in MDCK cells. The Rockefeller University Press 2005-08-15 /pmc/articles/PMC2171512/ /pubmed/16103222 http://dx.doi.org/10.1083/jcb.200503078 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Vieira, Otilia V.
Verkade, Paul
Manninen, Aki
Simons, Kai
FAPP2 is involved in the transport of apical cargo in polarized MDCK cells
title FAPP2 is involved in the transport of apical cargo in polarized MDCK cells
title_full FAPP2 is involved in the transport of apical cargo in polarized MDCK cells
title_fullStr FAPP2 is involved in the transport of apical cargo in polarized MDCK cells
title_full_unstemmed FAPP2 is involved in the transport of apical cargo in polarized MDCK cells
title_short FAPP2 is involved in the transport of apical cargo in polarized MDCK cells
title_sort fapp2 is involved in the transport of apical cargo in polarized mdck cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171512/
https://www.ncbi.nlm.nih.gov/pubmed/16103222
http://dx.doi.org/10.1083/jcb.200503078
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