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The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p

Palmitoylation of the vacuolar membrane protein Vac8p is essential for vacuole fusion in yeast (Veit, M., R. Laage, L. Dietrich, L. Wang, and C. Ungermann. 2001. EMBO J. 20:3145–3155; Wang, Y.X., E.J. Kauffman, J.E. Duex, and L.S. Weisman. 2001. J. Biol. Chem. 276:35133–35140). Proteins that contain...

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Detalles Bibliográficos
Autores principales: Smotrys, Jessica E., Schoenfish, Marissa J., Stutz, Monica A., Linder, Maurine E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171546/
https://www.ncbi.nlm.nih.gov/pubmed/16186255
http://dx.doi.org/10.1083/jcb.200507048
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author Smotrys, Jessica E.
Schoenfish, Marissa J.
Stutz, Monica A.
Linder, Maurine E.
author_facet Smotrys, Jessica E.
Schoenfish, Marissa J.
Stutz, Monica A.
Linder, Maurine E.
author_sort Smotrys, Jessica E.
collection PubMed
description Palmitoylation of the vacuolar membrane protein Vac8p is essential for vacuole fusion in yeast (Veit, M., R. Laage, L. Dietrich, L. Wang, and C. Ungermann. 2001. EMBO J. 20:3145–3155; Wang, Y.X., E.J. Kauffman, J.E. Duex, and L.S. Weisman. 2001. J. Biol. Chem. 276:35133–35140). Proteins that contain an Asp-His-His-Cys (DHHC)–cysteine rich domain (CRD) are emerging as a family of protein acyltransferases, and are therefore candidates for mediators of Vac8p palmitoylation. Here we demonstrate that the DHHC-CRD proteins Pfa3p (protein fatty acyltransferase 3, encoded by YNL326c) and Swf1p are important for vacuole fusion. Cells lacking Pfa3p had fragmented vacuoles when stressed, and cells lacking both Pfa3p and Swf1p had fragmented vacuoles under normal growth conditions. Pfa3p promoted Vac8p membrane association and palmitoylation in vivo and partially purified Pfa3p palmitoylated Vac8p in vitro, establishing Vac8p as a substrate for palmitoylation by Pfa3p. Vac8p is the first N-myristoylated, palmitoylated protein identified as a substrate for a DHHC-CRD protein.
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spelling pubmed-21715462008-03-05 The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p Smotrys, Jessica E. Schoenfish, Marissa J. Stutz, Monica A. Linder, Maurine E. J Cell Biol Research Articles Palmitoylation of the vacuolar membrane protein Vac8p is essential for vacuole fusion in yeast (Veit, M., R. Laage, L. Dietrich, L. Wang, and C. Ungermann. 2001. EMBO J. 20:3145–3155; Wang, Y.X., E.J. Kauffman, J.E. Duex, and L.S. Weisman. 2001. J. Biol. Chem. 276:35133–35140). Proteins that contain an Asp-His-His-Cys (DHHC)–cysteine rich domain (CRD) are emerging as a family of protein acyltransferases, and are therefore candidates for mediators of Vac8p palmitoylation. Here we demonstrate that the DHHC-CRD proteins Pfa3p (protein fatty acyltransferase 3, encoded by YNL326c) and Swf1p are important for vacuole fusion. Cells lacking Pfa3p had fragmented vacuoles when stressed, and cells lacking both Pfa3p and Swf1p had fragmented vacuoles under normal growth conditions. Pfa3p promoted Vac8p membrane association and palmitoylation in vivo and partially purified Pfa3p palmitoylated Vac8p in vitro, establishing Vac8p as a substrate for palmitoylation by Pfa3p. Vac8p is the first N-myristoylated, palmitoylated protein identified as a substrate for a DHHC-CRD protein. The Rockefeller University Press 2005-09-26 /pmc/articles/PMC2171546/ /pubmed/16186255 http://dx.doi.org/10.1083/jcb.200507048 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Smotrys, Jessica E.
Schoenfish, Marissa J.
Stutz, Monica A.
Linder, Maurine E.
The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p
title The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p
title_full The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p
title_fullStr The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p
title_full_unstemmed The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p
title_short The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p
title_sort vacuolar dhhc-crd protein pfa3p is a protein acyltransferase for vac8p
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171546/
https://www.ncbi.nlm.nih.gov/pubmed/16186255
http://dx.doi.org/10.1083/jcb.200507048
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