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The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p
Palmitoylation of the vacuolar membrane protein Vac8p is essential for vacuole fusion in yeast (Veit, M., R. Laage, L. Dietrich, L. Wang, and C. Ungermann. 2001. EMBO J. 20:3145–3155; Wang, Y.X., E.J. Kauffman, J.E. Duex, and L.S. Weisman. 2001. J. Biol. Chem. 276:35133–35140). Proteins that contain...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2005
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171546/ https://www.ncbi.nlm.nih.gov/pubmed/16186255 http://dx.doi.org/10.1083/jcb.200507048 |
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author | Smotrys, Jessica E. Schoenfish, Marissa J. Stutz, Monica A. Linder, Maurine E. |
author_facet | Smotrys, Jessica E. Schoenfish, Marissa J. Stutz, Monica A. Linder, Maurine E. |
author_sort | Smotrys, Jessica E. |
collection | PubMed |
description | Palmitoylation of the vacuolar membrane protein Vac8p is essential for vacuole fusion in yeast (Veit, M., R. Laage, L. Dietrich, L. Wang, and C. Ungermann. 2001. EMBO J. 20:3145–3155; Wang, Y.X., E.J. Kauffman, J.E. Duex, and L.S. Weisman. 2001. J. Biol. Chem. 276:35133–35140). Proteins that contain an Asp-His-His-Cys (DHHC)–cysteine rich domain (CRD) are emerging as a family of protein acyltransferases, and are therefore candidates for mediators of Vac8p palmitoylation. Here we demonstrate that the DHHC-CRD proteins Pfa3p (protein fatty acyltransferase 3, encoded by YNL326c) and Swf1p are important for vacuole fusion. Cells lacking Pfa3p had fragmented vacuoles when stressed, and cells lacking both Pfa3p and Swf1p had fragmented vacuoles under normal growth conditions. Pfa3p promoted Vac8p membrane association and palmitoylation in vivo and partially purified Pfa3p palmitoylated Vac8p in vitro, establishing Vac8p as a substrate for palmitoylation by Pfa3p. Vac8p is the first N-myristoylated, palmitoylated protein identified as a substrate for a DHHC-CRD protein. |
format | Text |
id | pubmed-2171546 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21715462008-03-05 The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p Smotrys, Jessica E. Schoenfish, Marissa J. Stutz, Monica A. Linder, Maurine E. J Cell Biol Research Articles Palmitoylation of the vacuolar membrane protein Vac8p is essential for vacuole fusion in yeast (Veit, M., R. Laage, L. Dietrich, L. Wang, and C. Ungermann. 2001. EMBO J. 20:3145–3155; Wang, Y.X., E.J. Kauffman, J.E. Duex, and L.S. Weisman. 2001. J. Biol. Chem. 276:35133–35140). Proteins that contain an Asp-His-His-Cys (DHHC)–cysteine rich domain (CRD) are emerging as a family of protein acyltransferases, and are therefore candidates for mediators of Vac8p palmitoylation. Here we demonstrate that the DHHC-CRD proteins Pfa3p (protein fatty acyltransferase 3, encoded by YNL326c) and Swf1p are important for vacuole fusion. Cells lacking Pfa3p had fragmented vacuoles when stressed, and cells lacking both Pfa3p and Swf1p had fragmented vacuoles under normal growth conditions. Pfa3p promoted Vac8p membrane association and palmitoylation in vivo and partially purified Pfa3p palmitoylated Vac8p in vitro, establishing Vac8p as a substrate for palmitoylation by Pfa3p. Vac8p is the first N-myristoylated, palmitoylated protein identified as a substrate for a DHHC-CRD protein. The Rockefeller University Press 2005-09-26 /pmc/articles/PMC2171546/ /pubmed/16186255 http://dx.doi.org/10.1083/jcb.200507048 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Smotrys, Jessica E. Schoenfish, Marissa J. Stutz, Monica A. Linder, Maurine E. The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p |
title | The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p |
title_full | The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p |
title_fullStr | The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p |
title_full_unstemmed | The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p |
title_short | The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p |
title_sort | vacuolar dhhc-crd protein pfa3p is a protein acyltransferase for vac8p |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171546/ https://www.ncbi.nlm.nih.gov/pubmed/16186255 http://dx.doi.org/10.1083/jcb.200507048 |
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