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The cell cycle–apoptosis connection revisited in the adult brain
Adult neurogenesis is studied in vivo using thymidine analogues such as bromodeoxyuridine (BrdU) to label DNA synthesis during the S phase of the cell cycle. However, BrdU may also label DNA synthesis events not directly related to cell proliferation, such as DNA repair and/or abortive reentry into...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2005
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171559/ https://www.ncbi.nlm.nih.gov/pubmed/16291699 http://dx.doi.org/10.1083/jcb.200505072 |
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author | Bauer, Sylvian Patterson, Paul H. |
author_facet | Bauer, Sylvian Patterson, Paul H. |
author_sort | Bauer, Sylvian |
collection | PubMed |
description | Adult neurogenesis is studied in vivo using thymidine analogues such as bromodeoxyuridine (BrdU) to label DNA synthesis during the S phase of the cell cycle. However, BrdU may also label DNA synthesis events not directly related to cell proliferation, such as DNA repair and/or abortive reentry into the cell cycle, which can occur as part of an apoptotic process in postmitotic neurons. In this study, we used three well-characterized models of injury-induced neuronal apoptosis and the combined visualization of cell birth (BrdU labeling) and death (Tdt-mediated dUTP-biotin nick end labeling) to investigate the specificity of BrdU incorporation in the adult mouse brain in vivo. We present evidence that BrdU is not significantly incorporated during DNA repair and that labeling is not detected in vulnerable or dying postmitotic neurons, even when a high dose of BrdU is directly infused into the brain. These findings have important implications for a controversy surrounding adult neurogenesis: the connection between cell cycle reactivation and apoptosis of terminally differentiated neurons. |
format | Text |
id | pubmed-2171559 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21715592008-03-05 The cell cycle–apoptosis connection revisited in the adult brain Bauer, Sylvian Patterson, Paul H. J Cell Biol Research Articles Adult neurogenesis is studied in vivo using thymidine analogues such as bromodeoxyuridine (BrdU) to label DNA synthesis during the S phase of the cell cycle. However, BrdU may also label DNA synthesis events not directly related to cell proliferation, such as DNA repair and/or abortive reentry into the cell cycle, which can occur as part of an apoptotic process in postmitotic neurons. In this study, we used three well-characterized models of injury-induced neuronal apoptosis and the combined visualization of cell birth (BrdU labeling) and death (Tdt-mediated dUTP-biotin nick end labeling) to investigate the specificity of BrdU incorporation in the adult mouse brain in vivo. We present evidence that BrdU is not significantly incorporated during DNA repair and that labeling is not detected in vulnerable or dying postmitotic neurons, even when a high dose of BrdU is directly infused into the brain. These findings have important implications for a controversy surrounding adult neurogenesis: the connection between cell cycle reactivation and apoptosis of terminally differentiated neurons. The Rockefeller University Press 2005-11-21 /pmc/articles/PMC2171559/ /pubmed/16291699 http://dx.doi.org/10.1083/jcb.200505072 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Bauer, Sylvian Patterson, Paul H. The cell cycle–apoptosis connection revisited in the adult brain |
title | The cell cycle–apoptosis connection revisited in the adult brain |
title_full | The cell cycle–apoptosis connection revisited in the adult brain |
title_fullStr | The cell cycle–apoptosis connection revisited in the adult brain |
title_full_unstemmed | The cell cycle–apoptosis connection revisited in the adult brain |
title_short | The cell cycle–apoptosis connection revisited in the adult brain |
title_sort | cell cycle–apoptosis connection revisited in the adult brain |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171559/ https://www.ncbi.nlm.nih.gov/pubmed/16291699 http://dx.doi.org/10.1083/jcb.200505072 |
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