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The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster

Conventional centrosomes are absent from the spindle in female meiosis in many species, but it is not clear how multiple chromosomes form one shared bipolar spindle without centrosomes. We identified a female sterile mutant in which each bivalent chromosome often forms a separate bipolar metaphase I...

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Detalles Bibliográficos
Autores principales: Cullen, C. Fiona, Brittle, Amy L., Ito, Takashi, Ohkura, Hiroyuki
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171570/
https://www.ncbi.nlm.nih.gov/pubmed/16301329
http://dx.doi.org/10.1083/jcb.200508127
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author Cullen, C. Fiona
Brittle, Amy L.
Ito, Takashi
Ohkura, Hiroyuki
author_facet Cullen, C. Fiona
Brittle, Amy L.
Ito, Takashi
Ohkura, Hiroyuki
author_sort Cullen, C. Fiona
collection PubMed
description Conventional centrosomes are absent from the spindle in female meiosis in many species, but it is not clear how multiple chromosomes form one shared bipolar spindle without centrosomes. We identified a female sterile mutant in which each bivalent chromosome often forms a separate bipolar metaphase I spindle. Unlike wild type, prophase I chromosomes fail to form a single compact structure within the oocyte nucleus, although the integrity of metaphase I chromosomes appears to be normal. Molecular analysis indicates that the mutant is defective in the conserved kinase nucleosomal histone kinase-1 (NHK-1). Isolation of further alleles and RNA interference in S2 cells demonstrated that NHK-1 is also required for mitotic progression. NHK-1 itself is phosphorylated in mitosis and female meiosis, suggesting that this kinase is part of the regulatory system coordinating progression of mitosis and meiosis.
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spelling pubmed-21715702008-03-05 The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster Cullen, C. Fiona Brittle, Amy L. Ito, Takashi Ohkura, Hiroyuki J Cell Biol Research Articles Conventional centrosomes are absent from the spindle in female meiosis in many species, but it is not clear how multiple chromosomes form one shared bipolar spindle without centrosomes. We identified a female sterile mutant in which each bivalent chromosome often forms a separate bipolar metaphase I spindle. Unlike wild type, prophase I chromosomes fail to form a single compact structure within the oocyte nucleus, although the integrity of metaphase I chromosomes appears to be normal. Molecular analysis indicates that the mutant is defective in the conserved kinase nucleosomal histone kinase-1 (NHK-1). Isolation of further alleles and RNA interference in S2 cells demonstrated that NHK-1 is also required for mitotic progression. NHK-1 itself is phosphorylated in mitosis and female meiosis, suggesting that this kinase is part of the regulatory system coordinating progression of mitosis and meiosis. The Rockefeller University Press 2005-11-21 /pmc/articles/PMC2171570/ /pubmed/16301329 http://dx.doi.org/10.1083/jcb.200508127 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Cullen, C. Fiona
Brittle, Amy L.
Ito, Takashi
Ohkura, Hiroyuki
The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster
title The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster
title_full The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster
title_fullStr The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster
title_full_unstemmed The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster
title_short The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster
title_sort conserved kinase nhk-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in drosophila melanogaster
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171570/
https://www.ncbi.nlm.nih.gov/pubmed/16301329
http://dx.doi.org/10.1083/jcb.200508127
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