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Generation of nonidentical compartments in vesicular transport systems
How can organelles communicate by bidirectional vesicle transport and yet maintain different protein compositions? We show by mathematical modeling that a minimal system, in which the basic variables are cytosolic coats for vesicle budding and membrane-bound soluble N-ethyl-maleimide–sensitive facto...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2005
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171580/ https://www.ncbi.nlm.nih.gov/pubmed/15657397 http://dx.doi.org/10.1083/jcb.200409087 |
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author | Heinrich, Reinhart Rapoport, Tom A. |
author_facet | Heinrich, Reinhart Rapoport, Tom A. |
author_sort | Heinrich, Reinhart |
collection | PubMed |
description | How can organelles communicate by bidirectional vesicle transport and yet maintain different protein compositions? We show by mathematical modeling that a minimal system, in which the basic variables are cytosolic coats for vesicle budding and membrane-bound soluble N-ethyl-maleimide–sensitive factor attachment protein receptors (SNAREs) for vesicle fusion, is sufficient to generate stable, nonidentical compartments. A requirement for establishing and maintaining distinct compartments is that each coat preferentially packages certain SNAREs during vesicle budding. Vesicles fuse preferentially with the compartment that contains the highest concentration of cognate SNAREs, thus further increasing these SNAREs. The stable steady state is the result of a balance between this autocatalytic SNARE accumulation in a compartment and the distribution of SNAREs between compartments by vesicle budding. The resulting nonhomogeneous SNARE distribution generates coat-specific vesicle fluxes that determine the size of compartments. With nonidentical compartments established in this way, the localization and cellular transport of cargo proteins can be explained simply by their affinity for coats. |
format | Text |
id | pubmed-2171580 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21715802008-03-05 Generation of nonidentical compartments in vesicular transport systems Heinrich, Reinhart Rapoport, Tom A. J Cell Biol Research Articles How can organelles communicate by bidirectional vesicle transport and yet maintain different protein compositions? We show by mathematical modeling that a minimal system, in which the basic variables are cytosolic coats for vesicle budding and membrane-bound soluble N-ethyl-maleimide–sensitive factor attachment protein receptors (SNAREs) for vesicle fusion, is sufficient to generate stable, nonidentical compartments. A requirement for establishing and maintaining distinct compartments is that each coat preferentially packages certain SNAREs during vesicle budding. Vesicles fuse preferentially with the compartment that contains the highest concentration of cognate SNAREs, thus further increasing these SNAREs. The stable steady state is the result of a balance between this autocatalytic SNARE accumulation in a compartment and the distribution of SNAREs between compartments by vesicle budding. The resulting nonhomogeneous SNARE distribution generates coat-specific vesicle fluxes that determine the size of compartments. With nonidentical compartments established in this way, the localization and cellular transport of cargo proteins can be explained simply by their affinity for coats. The Rockefeller University Press 2005-01-17 /pmc/articles/PMC2171580/ /pubmed/15657397 http://dx.doi.org/10.1083/jcb.200409087 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Heinrich, Reinhart Rapoport, Tom A. Generation of nonidentical compartments in vesicular transport systems |
title | Generation of nonidentical compartments in vesicular transport systems |
title_full | Generation of nonidentical compartments in vesicular transport systems |
title_fullStr | Generation of nonidentical compartments in vesicular transport systems |
title_full_unstemmed | Generation of nonidentical compartments in vesicular transport systems |
title_short | Generation of nonidentical compartments in vesicular transport systems |
title_sort | generation of nonidentical compartments in vesicular transport systems |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171580/ https://www.ncbi.nlm.nih.gov/pubmed/15657397 http://dx.doi.org/10.1083/jcb.200409087 |
work_keys_str_mv | AT heinrichreinhart generationofnonidenticalcompartmentsinvesiculartransportsystems AT rapoporttoma generationofnonidenticalcompartmentsinvesiculartransportsystems |