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ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs
Posttranscriptional, site-specific adenosine to inosine (A-to-I) base conversions, designated as RNA editing, play significant roles in generating diversity of gene expression. However, little is known about how and in which cellular compartments RNA editing is controlled. Interestingly, the two enz...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171610/ https://www.ncbi.nlm.nih.gov/pubmed/15939761 http://dx.doi.org/10.1083/jcb.200411129 |
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author | Vitali, Patrice Basyuk, Eugenia Le Meur, Elodie Bertrand, Edouard Muscatelli, Françoise Cavaillé, Jérôme Huttenhofer, Alexander |
author_facet | Vitali, Patrice Basyuk, Eugenia Le Meur, Elodie Bertrand, Edouard Muscatelli, Françoise Cavaillé, Jérôme Huttenhofer, Alexander |
author_sort | Vitali, Patrice |
collection | PubMed |
description | Posttranscriptional, site-specific adenosine to inosine (A-to-I) base conversions, designated as RNA editing, play significant roles in generating diversity of gene expression. However, little is known about how and in which cellular compartments RNA editing is controlled. Interestingly, the two enzymes that catalyze RNA editing, adenosine deaminases that act on RNA (ADAR) 1 and 2, have recently been demonstrated to dynamically associate with the nucleolus. Moreover, we have identified a brain-specific small RNA, termed MBII-52, which was predicted to function as a nucleolar C/D RNA, thereby targeting an A-to-I editing site (C-site) within the 5-HT2C serotonin receptor pre-mRNA for 2′-O-methylation. Through the subcellular targeting of minigenes that contain natural editing sites, we show that ADAR2- but not ADAR1-mediated RNA editing occurs in the nucleolus. We also demonstrate that MBII-52 forms a bona fide small nucleolar ribonucleoprotein particle that specifically decreases the efficiency of RNA editing by ADAR2 at the targeted C-site. Our data are consistent with a model in which C/D small nucleolar RNA might play a role in the regulation of RNA editing. |
format | Text |
id | pubmed-2171610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21716102008-03-05 ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs Vitali, Patrice Basyuk, Eugenia Le Meur, Elodie Bertrand, Edouard Muscatelli, Françoise Cavaillé, Jérôme Huttenhofer, Alexander J Cell Biol Research Articles Posttranscriptional, site-specific adenosine to inosine (A-to-I) base conversions, designated as RNA editing, play significant roles in generating diversity of gene expression. However, little is known about how and in which cellular compartments RNA editing is controlled. Interestingly, the two enzymes that catalyze RNA editing, adenosine deaminases that act on RNA (ADAR) 1 and 2, have recently been demonstrated to dynamically associate with the nucleolus. Moreover, we have identified a brain-specific small RNA, termed MBII-52, which was predicted to function as a nucleolar C/D RNA, thereby targeting an A-to-I editing site (C-site) within the 5-HT2C serotonin receptor pre-mRNA for 2′-O-methylation. Through the subcellular targeting of minigenes that contain natural editing sites, we show that ADAR2- but not ADAR1-mediated RNA editing occurs in the nucleolus. We also demonstrate that MBII-52 forms a bona fide small nucleolar ribonucleoprotein particle that specifically decreases the efficiency of RNA editing by ADAR2 at the targeted C-site. Our data are consistent with a model in which C/D small nucleolar RNA might play a role in the regulation of RNA editing. The Rockefeller University Press 2005-06-06 /pmc/articles/PMC2171610/ /pubmed/15939761 http://dx.doi.org/10.1083/jcb.200411129 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Vitali, Patrice Basyuk, Eugenia Le Meur, Elodie Bertrand, Edouard Muscatelli, Françoise Cavaillé, Jérôme Huttenhofer, Alexander ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs |
title | ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs |
title_full | ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs |
title_fullStr | ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs |
title_full_unstemmed | ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs |
title_short | ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs |
title_sort | adar2-mediated editing of rna substrates in the nucleolus is inhibited by c/d small nucleolar rnas |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171610/ https://www.ncbi.nlm.nih.gov/pubmed/15939761 http://dx.doi.org/10.1083/jcb.200411129 |
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