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ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs

Posttranscriptional, site-specific adenosine to inosine (A-to-I) base conversions, designated as RNA editing, play significant roles in generating diversity of gene expression. However, little is known about how and in which cellular compartments RNA editing is controlled. Interestingly, the two enz...

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Autores principales: Vitali, Patrice, Basyuk, Eugenia, Le Meur, Elodie, Bertrand, Edouard, Muscatelli, Françoise, Cavaillé, Jérôme, Huttenhofer, Alexander
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171610/
https://www.ncbi.nlm.nih.gov/pubmed/15939761
http://dx.doi.org/10.1083/jcb.200411129
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author Vitali, Patrice
Basyuk, Eugenia
Le Meur, Elodie
Bertrand, Edouard
Muscatelli, Françoise
Cavaillé, Jérôme
Huttenhofer, Alexander
author_facet Vitali, Patrice
Basyuk, Eugenia
Le Meur, Elodie
Bertrand, Edouard
Muscatelli, Françoise
Cavaillé, Jérôme
Huttenhofer, Alexander
author_sort Vitali, Patrice
collection PubMed
description Posttranscriptional, site-specific adenosine to inosine (A-to-I) base conversions, designated as RNA editing, play significant roles in generating diversity of gene expression. However, little is known about how and in which cellular compartments RNA editing is controlled. Interestingly, the two enzymes that catalyze RNA editing, adenosine deaminases that act on RNA (ADAR) 1 and 2, have recently been demonstrated to dynamically associate with the nucleolus. Moreover, we have identified a brain-specific small RNA, termed MBII-52, which was predicted to function as a nucleolar C/D RNA, thereby targeting an A-to-I editing site (C-site) within the 5-HT2C serotonin receptor pre-mRNA for 2′-O-methylation. Through the subcellular targeting of minigenes that contain natural editing sites, we show that ADAR2- but not ADAR1-mediated RNA editing occurs in the nucleolus. We also demonstrate that MBII-52 forms a bona fide small nucleolar ribonucleoprotein particle that specifically decreases the efficiency of RNA editing by ADAR2 at the targeted C-site. Our data are consistent with a model in which C/D small nucleolar RNA might play a role in the regulation of RNA editing.
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spelling pubmed-21716102008-03-05 ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs Vitali, Patrice Basyuk, Eugenia Le Meur, Elodie Bertrand, Edouard Muscatelli, Françoise Cavaillé, Jérôme Huttenhofer, Alexander J Cell Biol Research Articles Posttranscriptional, site-specific adenosine to inosine (A-to-I) base conversions, designated as RNA editing, play significant roles in generating diversity of gene expression. However, little is known about how and in which cellular compartments RNA editing is controlled. Interestingly, the two enzymes that catalyze RNA editing, adenosine deaminases that act on RNA (ADAR) 1 and 2, have recently been demonstrated to dynamically associate with the nucleolus. Moreover, we have identified a brain-specific small RNA, termed MBII-52, which was predicted to function as a nucleolar C/D RNA, thereby targeting an A-to-I editing site (C-site) within the 5-HT2C serotonin receptor pre-mRNA for 2′-O-methylation. Through the subcellular targeting of minigenes that contain natural editing sites, we show that ADAR2- but not ADAR1-mediated RNA editing occurs in the nucleolus. We also demonstrate that MBII-52 forms a bona fide small nucleolar ribonucleoprotein particle that specifically decreases the efficiency of RNA editing by ADAR2 at the targeted C-site. Our data are consistent with a model in which C/D small nucleolar RNA might play a role in the regulation of RNA editing. The Rockefeller University Press 2005-06-06 /pmc/articles/PMC2171610/ /pubmed/15939761 http://dx.doi.org/10.1083/jcb.200411129 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Vitali, Patrice
Basyuk, Eugenia
Le Meur, Elodie
Bertrand, Edouard
Muscatelli, Françoise
Cavaillé, Jérôme
Huttenhofer, Alexander
ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs
title ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs
title_full ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs
title_fullStr ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs
title_full_unstemmed ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs
title_short ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs
title_sort adar2-mediated editing of rna substrates in the nucleolus is inhibited by c/d small nucleolar rnas
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171610/
https://www.ncbi.nlm.nih.gov/pubmed/15939761
http://dx.doi.org/10.1083/jcb.200411129
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