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Centrosome fragments and microtubules are transported asymmetrically away from division plane in anaphase

Spinning disc confocal microscopy of LLCPK1 cells expressing GFP-tubulin was used to demonstrate that microtubules (MTs) rapidly elongate to the cell cortex after anaphase onset. Concurrently, individual MTs are released from the centrosome and the centrosome fragments into clusters of MTs. Using ce...

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Detalles Bibliográficos
Autores principales: Rusan, Nasser M., Wadsworth, Patricia
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171684/
https://www.ncbi.nlm.nih.gov/pubmed/15631988
http://dx.doi.org/10.1083/jcb.200409153
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author Rusan, Nasser M.
Wadsworth, Patricia
author_facet Rusan, Nasser M.
Wadsworth, Patricia
author_sort Rusan, Nasser M.
collection PubMed
description Spinning disc confocal microscopy of LLCPK1 cells expressing GFP-tubulin was used to demonstrate that microtubules (MTs) rapidly elongate to the cell cortex after anaphase onset. Concurrently, individual MTs are released from the centrosome and the centrosome fragments into clusters of MTs. Using cells expressing photoactivatable GFP-tubulin to mark centrosomal MT minus ends, a sevenfold increase in MT release in anaphase is documented as compared with metaphase. Transport of both individually released MTs and clusters of MTs is directionally biased: motion is directed away from the equatorial region. Clusters of MTs retain centrosomal components at their focus and the capacity to nucleate MTs. Injection of mRNA encoding nondegradable cyclin B blocked centrosome fragmentation and the stimulation of MT release in anaphase despite allowing anaphase-like chromosome segregation. Biased MT release may provide a mechanism for MT-dependent positioning of components necessary for specifying the site of contractile ring formation.
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spelling pubmed-21716842008-03-05 Centrosome fragments and microtubules are transported asymmetrically away from division plane in anaphase Rusan, Nasser M. Wadsworth, Patricia J Cell Biol Research Articles Spinning disc confocal microscopy of LLCPK1 cells expressing GFP-tubulin was used to demonstrate that microtubules (MTs) rapidly elongate to the cell cortex after anaphase onset. Concurrently, individual MTs are released from the centrosome and the centrosome fragments into clusters of MTs. Using cells expressing photoactivatable GFP-tubulin to mark centrosomal MT minus ends, a sevenfold increase in MT release in anaphase is documented as compared with metaphase. Transport of both individually released MTs and clusters of MTs is directionally biased: motion is directed away from the equatorial region. Clusters of MTs retain centrosomal components at their focus and the capacity to nucleate MTs. Injection of mRNA encoding nondegradable cyclin B blocked centrosome fragmentation and the stimulation of MT release in anaphase despite allowing anaphase-like chromosome segregation. Biased MT release may provide a mechanism for MT-dependent positioning of components necessary for specifying the site of contractile ring formation. The Rockefeller University Press 2005-01-03 /pmc/articles/PMC2171684/ /pubmed/15631988 http://dx.doi.org/10.1083/jcb.200409153 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Rusan, Nasser M.
Wadsworth, Patricia
Centrosome fragments and microtubules are transported asymmetrically away from division plane in anaphase
title Centrosome fragments and microtubules are transported asymmetrically away from division plane in anaphase
title_full Centrosome fragments and microtubules are transported asymmetrically away from division plane in anaphase
title_fullStr Centrosome fragments and microtubules are transported asymmetrically away from division plane in anaphase
title_full_unstemmed Centrosome fragments and microtubules are transported asymmetrically away from division plane in anaphase
title_short Centrosome fragments and microtubules are transported asymmetrically away from division plane in anaphase
title_sort centrosome fragments and microtubules are transported asymmetrically away from division plane in anaphase
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171684/
https://www.ncbi.nlm.nih.gov/pubmed/15631988
http://dx.doi.org/10.1083/jcb.200409153
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