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Inducible ablation of mouse Langerhans cells diminishes but fails to abrogate contact hypersensitivity
Langerhans cells (LC) form a unique subset of dendritic cells (DC) in the epidermis but so far their in vivo functions in skin immunity and tolerance could not be determined, in particular in relation to dermal DC (dDC). Here, we exploit a novel diphtheria toxin (DT) receptor (DTR)/DT-based system t...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171694/ https://www.ncbi.nlm.nih.gov/pubmed/15897263 http://dx.doi.org/10.1083/jcb.200501071 |
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author | Bennett, Clare L. van Rijn, Erwin Jung, Steffen Inaba, Kayo Steinman, Ralph M. Kapsenberg, Martien L. Clausen, Björn E. |
author_facet | Bennett, Clare L. van Rijn, Erwin Jung, Steffen Inaba, Kayo Steinman, Ralph M. Kapsenberg, Martien L. Clausen, Björn E. |
author_sort | Bennett, Clare L. |
collection | PubMed |
description | Langerhans cells (LC) form a unique subset of dendritic cells (DC) in the epidermis but so far their in vivo functions in skin immunity and tolerance could not be determined, in particular in relation to dermal DC (dDC). Here, we exploit a novel diphtheria toxin (DT) receptor (DTR)/DT-based system to achieve inducible ablation of LC without affecting the skin environment. Within 24 h after intra-peritoneal injection of DT into Langerin-DTR mice LC are completely depleted from the epidermis and only begin to return 4 wk later. LC deletion occurs by apoptosis in the absence of inflammation and, in particular, the dDC compartment is not affected. In LC-depleted mice contact hypersensitivity (CHS) responses are significantly decreased, although ear swelling still occurs indicating that dDC can mediate CHS when necessary. Our results establish Langerin-DTR mice as a unique tool to study LC function in the steady state and to explore their relative importance compared with dDC in orchestrating skin immunity and tolerance. |
format | Text |
id | pubmed-2171694 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21716942008-03-05 Inducible ablation of mouse Langerhans cells diminishes but fails to abrogate contact hypersensitivity Bennett, Clare L. van Rijn, Erwin Jung, Steffen Inaba, Kayo Steinman, Ralph M. Kapsenberg, Martien L. Clausen, Björn E. J Cell Biol Research Articles Langerhans cells (LC) form a unique subset of dendritic cells (DC) in the epidermis but so far their in vivo functions in skin immunity and tolerance could not be determined, in particular in relation to dermal DC (dDC). Here, we exploit a novel diphtheria toxin (DT) receptor (DTR)/DT-based system to achieve inducible ablation of LC without affecting the skin environment. Within 24 h after intra-peritoneal injection of DT into Langerin-DTR mice LC are completely depleted from the epidermis and only begin to return 4 wk later. LC deletion occurs by apoptosis in the absence of inflammation and, in particular, the dDC compartment is not affected. In LC-depleted mice contact hypersensitivity (CHS) responses are significantly decreased, although ear swelling still occurs indicating that dDC can mediate CHS when necessary. Our results establish Langerin-DTR mice as a unique tool to study LC function in the steady state and to explore their relative importance compared with dDC in orchestrating skin immunity and tolerance. The Rockefeller University Press 2005-05-23 /pmc/articles/PMC2171694/ /pubmed/15897263 http://dx.doi.org/10.1083/jcb.200501071 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Bennett, Clare L. van Rijn, Erwin Jung, Steffen Inaba, Kayo Steinman, Ralph M. Kapsenberg, Martien L. Clausen, Björn E. Inducible ablation of mouse Langerhans cells diminishes but fails to abrogate contact hypersensitivity |
title | Inducible ablation of mouse Langerhans cells diminishes but fails to abrogate contact hypersensitivity |
title_full | Inducible ablation of mouse Langerhans cells diminishes but fails to abrogate contact hypersensitivity |
title_fullStr | Inducible ablation of mouse Langerhans cells diminishes but fails to abrogate contact hypersensitivity |
title_full_unstemmed | Inducible ablation of mouse Langerhans cells diminishes but fails to abrogate contact hypersensitivity |
title_short | Inducible ablation of mouse Langerhans cells diminishes but fails to abrogate contact hypersensitivity |
title_sort | inducible ablation of mouse langerhans cells diminishes but fails to abrogate contact hypersensitivity |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171694/ https://www.ncbi.nlm.nih.gov/pubmed/15897263 http://dx.doi.org/10.1083/jcb.200501071 |
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