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Junctional membrane inositol 1,4,5-trisphosphate receptor complex coordinates sensitization of the silent EGF-induced Ca(2+) signaling

Ca(2+) is a highly versatile intracellular signal that regulates many different cellular processes, and cells have developed mechanisms to have exquisite control over Ca(2+) signaling. Epidermal growth factor (EGF), which fails to mobilize intracellular Ca(2+) when administrated alone, becomes capab...

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Autores principales: Hur, Eun-Mi, Park, Yong-Soo, Huh, Yang Hoon, Yoo, Seung Hyun, Woo, Kyung-Chul, Choi, Bo-Hwa, Kim, Kyong-Tai
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171708/
https://www.ncbi.nlm.nih.gov/pubmed/15911880
http://dx.doi.org/10.1083/jcb.200411034
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author Hur, Eun-Mi
Park, Yong-Soo
Huh, Yang Hoon
Yoo, Seung Hyun
Woo, Kyung-Chul
Choi, Bo-Hwa
Kim, Kyong-Tai
author_facet Hur, Eun-Mi
Park, Yong-Soo
Huh, Yang Hoon
Yoo, Seung Hyun
Woo, Kyung-Chul
Choi, Bo-Hwa
Kim, Kyong-Tai
author_sort Hur, Eun-Mi
collection PubMed
description Ca(2+) is a highly versatile intracellular signal that regulates many different cellular processes, and cells have developed mechanisms to have exquisite control over Ca(2+) signaling. Epidermal growth factor (EGF), which fails to mobilize intracellular Ca(2+) when administrated alone, becomes capable of evoking [Ca(2+)](i) increase and exocytosis after bradykinin (BK) stimulation in chromaffin cells. Here, we provide evidence that this sensitization process is coordinated by a macromolecular signaling complex comprised of inositol 1,4,5-trisphosphate receptor type I (IP(3)R1), cAMP-dependent protein kinase (PKA), EGF receptor (EGFR), and an A-kinase anchoring protein, yotiao. The IP(3)R complex functions as a focal point to promote Ca(2+) release in two ways: (1) it facilitates PKA-dependent phosphorylation of IP(3)R1 in response to BK-induced elevation of cAMP, and (2) it couples the plasmalemmal EGFR with IP(3)R1 at the Ca(2+) store located juxtaposed to the plasma membrane. Our study illustrates how the junctional membrane IP(3)R complex connects different signaling pathways to define the fidelity and specificity of Ca(2+) signaling.
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spelling pubmed-21717082008-03-05 Junctional membrane inositol 1,4,5-trisphosphate receptor complex coordinates sensitization of the silent EGF-induced Ca(2+) signaling Hur, Eun-Mi Park, Yong-Soo Huh, Yang Hoon Yoo, Seung Hyun Woo, Kyung-Chul Choi, Bo-Hwa Kim, Kyong-Tai J Cell Biol Research Articles Ca(2+) is a highly versatile intracellular signal that regulates many different cellular processes, and cells have developed mechanisms to have exquisite control over Ca(2+) signaling. Epidermal growth factor (EGF), which fails to mobilize intracellular Ca(2+) when administrated alone, becomes capable of evoking [Ca(2+)](i) increase and exocytosis after bradykinin (BK) stimulation in chromaffin cells. Here, we provide evidence that this sensitization process is coordinated by a macromolecular signaling complex comprised of inositol 1,4,5-trisphosphate receptor type I (IP(3)R1), cAMP-dependent protein kinase (PKA), EGF receptor (EGFR), and an A-kinase anchoring protein, yotiao. The IP(3)R complex functions as a focal point to promote Ca(2+) release in two ways: (1) it facilitates PKA-dependent phosphorylation of IP(3)R1 in response to BK-induced elevation of cAMP, and (2) it couples the plasmalemmal EGFR with IP(3)R1 at the Ca(2+) store located juxtaposed to the plasma membrane. Our study illustrates how the junctional membrane IP(3)R complex connects different signaling pathways to define the fidelity and specificity of Ca(2+) signaling. The Rockefeller University Press 2005-05-23 /pmc/articles/PMC2171708/ /pubmed/15911880 http://dx.doi.org/10.1083/jcb.200411034 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Hur, Eun-Mi
Park, Yong-Soo
Huh, Yang Hoon
Yoo, Seung Hyun
Woo, Kyung-Chul
Choi, Bo-Hwa
Kim, Kyong-Tai
Junctional membrane inositol 1,4,5-trisphosphate receptor complex coordinates sensitization of the silent EGF-induced Ca(2+) signaling
title Junctional membrane inositol 1,4,5-trisphosphate receptor complex coordinates sensitization of the silent EGF-induced Ca(2+) signaling
title_full Junctional membrane inositol 1,4,5-trisphosphate receptor complex coordinates sensitization of the silent EGF-induced Ca(2+) signaling
title_fullStr Junctional membrane inositol 1,4,5-trisphosphate receptor complex coordinates sensitization of the silent EGF-induced Ca(2+) signaling
title_full_unstemmed Junctional membrane inositol 1,4,5-trisphosphate receptor complex coordinates sensitization of the silent EGF-induced Ca(2+) signaling
title_short Junctional membrane inositol 1,4,5-trisphosphate receptor complex coordinates sensitization of the silent EGF-induced Ca(2+) signaling
title_sort junctional membrane inositol 1,4,5-trisphosphate receptor complex coordinates sensitization of the silent egf-induced ca(2+) signaling
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171708/
https://www.ncbi.nlm.nih.gov/pubmed/15911880
http://dx.doi.org/10.1083/jcb.200411034
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