Cargando…

DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo

Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization of the actin cytoskeleton that is mediated by small GTPases of the Rho family. Often, Rho1 controls different aspects of cytoskeletal function in parallel, requiring a complex level of regulation. We show...

Descripción completa

Detalles Bibliográficos
Autores principales: Padash Barmchi, Mojgan, Rogers, Stephen, Häcker, Udo
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171764/
https://www.ncbi.nlm.nih.gov/pubmed/15699213
http://dx.doi.org/10.1083/jcb.200407124
_version_ 1782144970729193472
author Padash Barmchi, Mojgan
Rogers, Stephen
Häcker, Udo
author_facet Padash Barmchi, Mojgan
Rogers, Stephen
Häcker, Udo
author_sort Padash Barmchi, Mojgan
collection PubMed
description Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization of the actin cytoskeleton that is mediated by small GTPases of the Rho family. Often, Rho1 controls different aspects of cytoskeletal function in parallel, requiring a complex level of regulation. We show that the guanine triphosphate (GTP) exchange factor DRhoGEF2 is apically localized in epithelial cells throughout embryogenesis. We demonstrate that DRhoGEF2, which has previously been shown to regulate cell shape changes during gastrulation, recruits Rho1 to actin rings and regulates actin distribution and actomyosin contractility during nuclear divisions, pole cell formation, and cellularization of syncytial blastoderm embryos. We propose that DRhoGEF2 activity coordinates contractile actomyosin forces throughout morphogenesis in Drosophila by regulating the association of myosin with actin to form contractile cables. Our results support the hypothesis that specific aspects of Rho1 function are regulated by specific GTP exchange factors.
format Text
id pubmed-2171764
institution National Center for Biotechnology Information
language English
publishDate 2005
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21717642008-03-05 DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo Padash Barmchi, Mojgan Rogers, Stephen Häcker, Udo J Cell Biol Research Articles Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization of the actin cytoskeleton that is mediated by small GTPases of the Rho family. Often, Rho1 controls different aspects of cytoskeletal function in parallel, requiring a complex level of regulation. We show that the guanine triphosphate (GTP) exchange factor DRhoGEF2 is apically localized in epithelial cells throughout embryogenesis. We demonstrate that DRhoGEF2, which has previously been shown to regulate cell shape changes during gastrulation, recruits Rho1 to actin rings and regulates actin distribution and actomyosin contractility during nuclear divisions, pole cell formation, and cellularization of syncytial blastoderm embryos. We propose that DRhoGEF2 activity coordinates contractile actomyosin forces throughout morphogenesis in Drosophila by regulating the association of myosin with actin to form contractile cables. Our results support the hypothesis that specific aspects of Rho1 function are regulated by specific GTP exchange factors. The Rockefeller University Press 2005-02-14 /pmc/articles/PMC2171764/ /pubmed/15699213 http://dx.doi.org/10.1083/jcb.200407124 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Padash Barmchi, Mojgan
Rogers, Stephen
Häcker, Udo
DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo
title DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo
title_full DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo
title_fullStr DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo
title_full_unstemmed DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo
title_short DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo
title_sort drhogef2 regulates actin organization and contractility in the drosophila blastoderm embryo
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171764/
https://www.ncbi.nlm.nih.gov/pubmed/15699213
http://dx.doi.org/10.1083/jcb.200407124
work_keys_str_mv AT padashbarmchimojgan drhogef2regulatesactinorganizationandcontractilityinthedrosophilablastodermembryo
AT rogersstephen drhogef2regulatesactinorganizationandcontractilityinthedrosophilablastodermembryo
AT hackerudo drhogef2regulatesactinorganizationandcontractilityinthedrosophilablastodermembryo