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DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo
Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization of the actin cytoskeleton that is mediated by small GTPases of the Rho family. Often, Rho1 controls different aspects of cytoskeletal function in parallel, requiring a complex level of regulation. We show...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171764/ https://www.ncbi.nlm.nih.gov/pubmed/15699213 http://dx.doi.org/10.1083/jcb.200407124 |
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author | Padash Barmchi, Mojgan Rogers, Stephen Häcker, Udo |
author_facet | Padash Barmchi, Mojgan Rogers, Stephen Häcker, Udo |
author_sort | Padash Barmchi, Mojgan |
collection | PubMed |
description | Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization of the actin cytoskeleton that is mediated by small GTPases of the Rho family. Often, Rho1 controls different aspects of cytoskeletal function in parallel, requiring a complex level of regulation. We show that the guanine triphosphate (GTP) exchange factor DRhoGEF2 is apically localized in epithelial cells throughout embryogenesis. We demonstrate that DRhoGEF2, which has previously been shown to regulate cell shape changes during gastrulation, recruits Rho1 to actin rings and regulates actin distribution and actomyosin contractility during nuclear divisions, pole cell formation, and cellularization of syncytial blastoderm embryos. We propose that DRhoGEF2 activity coordinates contractile actomyosin forces throughout morphogenesis in Drosophila by regulating the association of myosin with actin to form contractile cables. Our results support the hypothesis that specific aspects of Rho1 function are regulated by specific GTP exchange factors. |
format | Text |
id | pubmed-2171764 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21717642008-03-05 DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo Padash Barmchi, Mojgan Rogers, Stephen Häcker, Udo J Cell Biol Research Articles Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization of the actin cytoskeleton that is mediated by small GTPases of the Rho family. Often, Rho1 controls different aspects of cytoskeletal function in parallel, requiring a complex level of regulation. We show that the guanine triphosphate (GTP) exchange factor DRhoGEF2 is apically localized in epithelial cells throughout embryogenesis. We demonstrate that DRhoGEF2, which has previously been shown to regulate cell shape changes during gastrulation, recruits Rho1 to actin rings and regulates actin distribution and actomyosin contractility during nuclear divisions, pole cell formation, and cellularization of syncytial blastoderm embryos. We propose that DRhoGEF2 activity coordinates contractile actomyosin forces throughout morphogenesis in Drosophila by regulating the association of myosin with actin to form contractile cables. Our results support the hypothesis that specific aspects of Rho1 function are regulated by specific GTP exchange factors. The Rockefeller University Press 2005-02-14 /pmc/articles/PMC2171764/ /pubmed/15699213 http://dx.doi.org/10.1083/jcb.200407124 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Padash Barmchi, Mojgan Rogers, Stephen Häcker, Udo DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo |
title |
DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo |
title_full |
DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo |
title_fullStr |
DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo |
title_full_unstemmed |
DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo |
title_short |
DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo |
title_sort | drhogef2 regulates actin organization and contractility in the drosophila blastoderm embryo |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171764/ https://www.ncbi.nlm.nih.gov/pubmed/15699213 http://dx.doi.org/10.1083/jcb.200407124 |
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