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PATJ regulates tight junction formation and polarity in mammalian epithelial cells

Recent studies have revealed an important role for tight junction protein complexes in epithelial cell polarity. One of these complexes contains the apical transmembrane protein, Crumbs, and two PSD95/discs large/zonula occludens domain proteins, protein associated with Lin seven 1 (PALS1)/Stardust...

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Detalles Bibliográficos
Autores principales: Shin, Kunyoo, Straight, Sam, Margolis, Ben
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171825/
https://www.ncbi.nlm.nih.gov/pubmed/15738264
http://dx.doi.org/10.1083/jcb.200408064
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author Shin, Kunyoo
Straight, Sam
Margolis, Ben
author_facet Shin, Kunyoo
Straight, Sam
Margolis, Ben
author_sort Shin, Kunyoo
collection PubMed
description Recent studies have revealed an important role for tight junction protein complexes in epithelial cell polarity. One of these complexes contains the apical transmembrane protein, Crumbs, and two PSD95/discs large/zonula occludens domain proteins, protein associated with Lin seven 1 (PALS1)/Stardust and PALS1-associated tight junction protein (PATJ). Although Crumbs and PALS1/Stardust are known to be important for cell polarization, recent studies have suggested that Drosophila PATJ is not essential and its function is unclear. Here, we find that PATJ is targeted to the apical region and tight junctions once cell polarization is initiated. We show using RNAi techniques that reduction in PATJ expression leads to delayed tight junction formation as well as defects in cell polarization. These effects are reversed by reintroduction of PATJ into these RNAi cells. This study provides new functional information on PATJ as a polarity protein and increases our understanding of the Crumbs–PALS1–PATJ complex function in epithelial polarity.
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spelling pubmed-21718252008-03-05 PATJ regulates tight junction formation and polarity in mammalian epithelial cells Shin, Kunyoo Straight, Sam Margolis, Ben J Cell Biol Research Articles Recent studies have revealed an important role for tight junction protein complexes in epithelial cell polarity. One of these complexes contains the apical transmembrane protein, Crumbs, and two PSD95/discs large/zonula occludens domain proteins, protein associated with Lin seven 1 (PALS1)/Stardust and PALS1-associated tight junction protein (PATJ). Although Crumbs and PALS1/Stardust are known to be important for cell polarization, recent studies have suggested that Drosophila PATJ is not essential and its function is unclear. Here, we find that PATJ is targeted to the apical region and tight junctions once cell polarization is initiated. We show using RNAi techniques that reduction in PATJ expression leads to delayed tight junction formation as well as defects in cell polarization. These effects are reversed by reintroduction of PATJ into these RNAi cells. This study provides new functional information on PATJ as a polarity protein and increases our understanding of the Crumbs–PALS1–PATJ complex function in epithelial polarity. The Rockefeller University Press 2005-02-28 /pmc/articles/PMC2171825/ /pubmed/15738264 http://dx.doi.org/10.1083/jcb.200408064 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Shin, Kunyoo
Straight, Sam
Margolis, Ben
PATJ regulates tight junction formation and polarity in mammalian epithelial cells
title PATJ regulates tight junction formation and polarity in mammalian epithelial cells
title_full PATJ regulates tight junction formation and polarity in mammalian epithelial cells
title_fullStr PATJ regulates tight junction formation and polarity in mammalian epithelial cells
title_full_unstemmed PATJ regulates tight junction formation and polarity in mammalian epithelial cells
title_short PATJ regulates tight junction formation and polarity in mammalian epithelial cells
title_sort patj regulates tight junction formation and polarity in mammalian epithelial cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171825/
https://www.ncbi.nlm.nih.gov/pubmed/15738264
http://dx.doi.org/10.1083/jcb.200408064
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