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Neural crest stem cell maintenance by combinatorial Wnt and BMP signaling
Canonical Wnt signaling instructively promotes sensory neurogenesis in early neural crest stem cells (eNCSCs) (Lee, H.Y., M. Kléber, L. Hari, V. Brault, U. Suter, M.M. Taketo, R. Kemler, and L. Sommer. 2004. Science. 303:1020–1023). However, during normal development Wnt signaling induces a sensory...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171862/ https://www.ncbi.nlm.nih.gov/pubmed/15837799 http://dx.doi.org/10.1083/jcb.200411095 |
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author | Kléber, Maurice Lee, Hye-Youn Wurdak, Heiko Buchstaller, Johanna Riccomagno, Martin M. Ittner, Lars M. Suter, Ueli Epstein, Douglas J. Sommer, Lukas |
author_facet | Kléber, Maurice Lee, Hye-Youn Wurdak, Heiko Buchstaller, Johanna Riccomagno, Martin M. Ittner, Lars M. Suter, Ueli Epstein, Douglas J. Sommer, Lukas |
author_sort | Kléber, Maurice |
collection | PubMed |
description | Canonical Wnt signaling instructively promotes sensory neurogenesis in early neural crest stem cells (eNCSCs) (Lee, H.Y., M. Kléber, L. Hari, V. Brault, U. Suter, M.M. Taketo, R. Kemler, and L. Sommer. 2004. Science. 303:1020–1023). However, during normal development Wnt signaling induces a sensory fate only in a subpopulation of eNCSCs while other cells maintain their stem cell features, despite the presence of Wnt activity. Hence, factors counteracting Wnt signaling must exist. Here, we show that bone morphogenic protein (BMP) signaling antagonizes the sensory fate-inducing activity of Wnt/β-catenin. Intriguingly, Wnt and BMP act synergistically to suppress differentiation and to maintain NCSC marker expression and multipotency. Similar to NCSCs in vivo, NCSCs maintained in culture alter their responsiveness to instructive growth factors with time. Thus, stem cell development is regulated by combinatorial growth factor activities that interact with changing cell-intrinsic cues. |
format | Text |
id | pubmed-2171862 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21718622008-03-05 Neural crest stem cell maintenance by combinatorial Wnt and BMP signaling Kléber, Maurice Lee, Hye-Youn Wurdak, Heiko Buchstaller, Johanna Riccomagno, Martin M. Ittner, Lars M. Suter, Ueli Epstein, Douglas J. Sommer, Lukas J Cell Biol Research Articles Canonical Wnt signaling instructively promotes sensory neurogenesis in early neural crest stem cells (eNCSCs) (Lee, H.Y., M. Kléber, L. Hari, V. Brault, U. Suter, M.M. Taketo, R. Kemler, and L. Sommer. 2004. Science. 303:1020–1023). However, during normal development Wnt signaling induces a sensory fate only in a subpopulation of eNCSCs while other cells maintain their stem cell features, despite the presence of Wnt activity. Hence, factors counteracting Wnt signaling must exist. Here, we show that bone morphogenic protein (BMP) signaling antagonizes the sensory fate-inducing activity of Wnt/β-catenin. Intriguingly, Wnt and BMP act synergistically to suppress differentiation and to maintain NCSC marker expression and multipotency. Similar to NCSCs in vivo, NCSCs maintained in culture alter their responsiveness to instructive growth factors with time. Thus, stem cell development is regulated by combinatorial growth factor activities that interact with changing cell-intrinsic cues. The Rockefeller University Press 2005-04-25 /pmc/articles/PMC2171862/ /pubmed/15837799 http://dx.doi.org/10.1083/jcb.200411095 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Kléber, Maurice Lee, Hye-Youn Wurdak, Heiko Buchstaller, Johanna Riccomagno, Martin M. Ittner, Lars M. Suter, Ueli Epstein, Douglas J. Sommer, Lukas Neural crest stem cell maintenance by combinatorial Wnt and BMP signaling |
title | Neural crest stem cell maintenance by combinatorial Wnt and BMP signaling |
title_full | Neural crest stem cell maintenance by combinatorial Wnt and BMP signaling |
title_fullStr | Neural crest stem cell maintenance by combinatorial Wnt and BMP signaling |
title_full_unstemmed | Neural crest stem cell maintenance by combinatorial Wnt and BMP signaling |
title_short | Neural crest stem cell maintenance by combinatorial Wnt and BMP signaling |
title_sort | neural crest stem cell maintenance by combinatorial wnt and bmp signaling |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171862/ https://www.ncbi.nlm.nih.gov/pubmed/15837799 http://dx.doi.org/10.1083/jcb.200411095 |
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